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Entacapone improves saccharification without affecting lignin and maize growth: An in silico, in vitro, and in vivo approach.

التفاصيل البيبلوغرافية
العنوان: Entacapone improves saccharification without affecting lignin and maize growth: An in silico, in vitro, and in vivo approach.
المؤلفون: Parizotto AV; Laboratory of Plant Biochemistry, Department of Biochemistry, University of Maringá, 87020-900, PR, Brazil., Ferro AP; Laboratory of Plant Biochemistry, Department of Biochemistry, University of Maringá, 87020-900, PR, Brazil., Marchiosi R; Laboratory of Plant Biochemistry, Department of Biochemistry, University of Maringá, 87020-900, PR, Brazil., Moreira-Vilar FC; Laboratory of Plant Biochemistry, Department of Biochemistry, University of Maringá, 87020-900, PR, Brazil., Bevilaqua JM; Laboratory of Plant Biochemistry, Department of Biochemistry, University of Maringá, 87020-900, PR, Brazil., Dos Santos WD; Laboratory of Plant Biochemistry, Department of Biochemistry, University of Maringá, 87020-900, PR, Brazil., Seixas FAV; Laboratory of Structural Biochemistry, Department of Technology, University of Maringá, Umuarama, 87506-370, PR, Brazil., Ferrarese-Filho O; Laboratory of Plant Biochemistry, Department of Biochemistry, University of Maringá, 87020-900, PR, Brazil. Electronic address: oferrarese@uem.br.
المصدر: Plant physiology and biochemistry : PPB [Plant Physiol Biochem] 2020 Jun; Vol. 151, pp. 421-428. Date of Electronic Publication: 2020 Apr 03.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Science Country of Publication: France NLM ID: 9882449 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2690 (Electronic) Linking ISSN: 09819428 NLM ISO Abbreviation: Plant Physiol Biochem Subsets: MEDLINE
أسماء مطبوعة: Publication: Amsterdam : Elsevier Science
Original Publication: Paris : Gauthier-Villars, c1987-
مواضيع طبية MeSH: Catechols*/pharmacology , Lignin* , Nitriles*/pharmacology , Polysaccharides*/metabolism , Zea mays*/drug effects, Biofuels ; Biomass ; Catechol O-Methyltransferase Inhibitors/pharmacology ; Cell Wall/drug effects ; Plants, Genetically Modified
مستخلص: Caffeate 3-O-methyltransferase (COMT) catalyzes the methylation of the 3-hydroxyl group of caffeate to produce ferulate, an important precursor of the lignin biosynthesis. As a crucial drawback for biofuel production, lignin limits the enzymatic hydrolysis of polysaccharides to result in fermentable sugars. We hypothesized that a controlled inhibition of maize COMT can be an efficient approach to reduce ferulate and lignin, thus improving the saccharification process. First, we applied in silico techniques to prospect potential inhibitors of ZmaysCOMT, and the nitrocatechol entacapone was selected. Second, in vitro assays confirmed the inhibitory effect of entacapone on maize COMT. Finally, in vivo experiments revealed that entacapone reduced the contents of cell-wall-esterified hydroxycinnamates and increased saccharification of stems (18%) and leaves (70%), without negatively affecting maize growth and lignin biosynthesis. This non-genetically modified approach can be an alternative strategy to facilitate the enzymatic hydrolysis of biomass polysaccharides and increase saccharification for bioethanol production.
Competing Interests: Declaration of competing interest The authors declare no conflicts of interest.
(Copyright © 2020 Elsevier Masson SAS. All rights reserved.)
فهرسة مساهمة: Keywords: Bioinformatics; Biomass digestibility; COMT; Hydroxycinnamates; Phenylpropanoid pathway
المشرفين على المادة: 0 (Biofuels)
0 (Catechol O-Methyltransferase Inhibitors)
0 (Catechols)
0 (Nitriles)
0 (Polysaccharides)
4975G9NM6T (entacapone)
9005-53-2 (Lignin)
تواريخ الأحداث: Date Created: 20200415 Date Completed: 20200728 Latest Revision: 20200930
رمز التحديث: 20221213
DOI: 10.1016/j.plaphy.2020.03.053
PMID: 32289635
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-2690
DOI:10.1016/j.plaphy.2020.03.053