دورية أكاديمية
أعرض في EDS

Methods for mechanical delivery of viral vectors into rhesus monkey brain.

التفاصيل البيبلوغرافية
العنوان: Methods for mechanical delivery of viral vectors into rhesus monkey brain.
المؤلفون: Fredericks JM; Laboratory of Neuropsychology, NIMH/NIH/DHHS, Bethesda, MD, USA; Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, New York, NY 10014, USA., Dash KE; Laboratory of Neuropsychology, NIMH/NIH/DHHS, Bethesda, MD, USA., Jaskot EM; Laboratory of Neuropsychology, NIMH/NIH/DHHS, Bethesda, MD, USA., Bennett TW; Section on Instrumentation, NIH/DHHS, Bethesda, MD, USA., Lerchner W; Laboratory of Neuropsychology, NIMH/NIH/DHHS, Bethesda, MD, USA., Dold G; Section on Instrumentation, NIH/DHHS, Bethesda, MD, USA., Ide D; Section on Instrumentation, NIH/DHHS, Bethesda, MD, USA., Cummins AC; Laboratory of Neuropsychology, NIMH/NIH/DHHS, Bethesda, MD, USA., Der Minassian VH; Laboratory of Neuropsychology, NIMH/NIH/DHHS, Bethesda, MD, USA., Turchi JN; Laboratory of Neuropsychology, NIMH/NIH/DHHS, Bethesda, MD, USA., Richmond BJ; Laboratory of Neuropsychology, NIMH/NIH/DHHS, Bethesda, MD, USA., Eldridge MAG; Laboratory of Neuropsychology, NIMH/NIH/DHHS, Bethesda, MD, USA. Electronic address: mark.eldridge@nih.gov.
المصدر: Journal of neuroscience methods [J Neurosci Methods] 2020 Jun 01; Vol. 339, pp. 108730. Date of Electronic Publication: 2020 Apr 14.
نوع المنشور: Journal Article; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 7905558 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-678X (Electronic) Linking ISSN: 01650270 NLM ISO Abbreviation: J Neurosci Methods Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam, Elsevier/North-Holland Biomedical Press.
مواضيع طبية MeSH: Brain* , Genetic Vectors*, Animals ; Macaca mulatta ; Optogenetics ; Reproducibility of Results
مستخلص: Background: Modern molecular tools make it possible to manipulate neural activity in a reversible and cell-type specific manner. For rhesus monkey research, molecular tools are generally introduced via viral vectors. New instruments designed specifically for use in monkey research are needed to enhance the efficiency and reliability of vector delivery.
New Method: A suite of multi-channel injection devices was developed to permit efficient and uniform vector delivery to cortical regions of the monkey brain. Manganese was co-infused with virus to allow rapid post-surgical confirmation of targeting accuracy using MRI. A needle guide was designed to increase the accuracy of sub-cortical targeting using stereotaxic co-ordinates.
Results: The multi-channel injection devices produced dense, uniform coverage of dorsal surface cortex, ventral surface cortex, and intra-sulcal cortex, respectively. Co-infusion of manganese with the viral vector allowed for immediate verification of injection accuracy. The needle guide improved accuracy of targeting sub-cortical structures by preventing needle deflection.
Comparison With Existing Method(s): The current methods, hand-held injections or single slow mechanical injection, for surface cortex transduction do not, in our hands, produce the density and uniformity of coverage provided by the injector arrays and associated infusion protocol.
Conclusions: The efficiency and reliability of vector delivery has been considerably improved by the development of new methods and instruments. This development should facilitate the translation of chemo- and optogenetic studies performed in smaller animals to larger animals such as rhesus monkeys.
Competing Interests: Declaration of Competing Interest No competing interests declared
(Published by Elsevier B.V.)
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معلومات مُعتمدة: Z01 MH002619 United States ImNIH Intramural NIH HHS; ZIA MH002619 United States ImNIH Intramural NIH HHS
فهرسة مساهمة: Keywords: Chemogenetic; DREADD; Lentivirus; Non-human primates; Optogenetic; Rhesus monkey
تواريخ الأحداث: Date Created: 20200418 Date Completed: 20210621 Latest Revision: 20210621
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC7238764
DOI: 10.1016/j.jneumeth.2020.108730
PMID: 32302596
قاعدة البيانات: MEDLINE
الوصف
تدمد:1872-678X
DOI:10.1016/j.jneumeth.2020.108730