دورية أكاديمية
Integrated scRNA-Seq Identifies Human Postnatal Thymus Seeding Progenitors and Regulatory Dynamics of Differentiating Immature Thymocytes.
| العنوان: | Integrated scRNA-Seq Identifies Human Postnatal Thymus Seeding Progenitors and Regulatory Dynamics of Differentiating Immature Thymocytes. |
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| المؤلفون: | Lavaert M; Faculty of Medicine and Health Sciences, Department of Diagnostic Sciences, Ghent University, C. Heymanslaan 10, MRB2, Entrance 38, 9000 Ghent, Belgium., Liang KL; Faculty of Medicine and Health Sciences, Department of Diagnostic Sciences, Ghent University, C. Heymanslaan 10, MRB2, Entrance 38, 9000 Ghent, Belgium., Vandamme N; Data Mining and Modeling for Biomedicine, VIB Center for Inflammation Research, Ghent, Belgium; Cancer Research Institute Ghent (CRIG), Ghent University, Ghent, Belgium., Park JE; Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SA, UK., Roels J; Faculty of Medicine and Health Sciences, Department of Diagnostic Sciences, Ghent University, C. Heymanslaan 10, MRB2, Entrance 38, 9000 Ghent, Belgium; Department of Biomolecular Medicine, Ghent University, Ghent, Belgium., Kowalczyk MS; Klarman Cell Observatory, Broad Institute of Harvard and MIT, Cambridge, MA, USA., Li B; Klarman Cell Observatory, Broad Institute of Harvard and MIT, Cambridge, MA, USA; Data Sciences Platform, Broad Institute of Harvard and MIT, Cambridge, MA, USA., Ashenberg O; Klarman Cell Observatory, Broad Institute of Harvard and MIT, Cambridge, MA, USA., Tabaka M; Klarman Cell Observatory, Broad Institute of Harvard and MIT, Cambridge, MA, USA., Dionne D; Klarman Cell Observatory, Broad Institute of Harvard and MIT, Cambridge, MA, USA., Tickle TL; Klarman Cell Observatory, Broad Institute of Harvard and MIT, Cambridge, MA, USA; Haematology Department, Royal Victoria Infirmary, Newcastle-upon-Tyne Hospitals NHS Foundation Trust, Newcastle upon Tyne, UK., Slyper M; Klarman Cell Observatory, Broad Institute of Harvard and MIT, Cambridge, MA, USA., Rozenblatt-Rosen O; Klarman Cell Observatory, Broad Institute of Harvard and MIT, Cambridge, MA, USA., Vandekerckhove B; Faculty of Medicine and Health Sciences, Department of Diagnostic Sciences, Ghent University, C. Heymanslaan 10, MRB2, Entrance 38, 9000 Ghent, Belgium; Cancer Research Institute Ghent (CRIG), Ghent University, Ghent, Belgium., Leclercq G; Faculty of Medicine and Health Sciences, Department of Diagnostic Sciences, Ghent University, C. Heymanslaan 10, MRB2, Entrance 38, 9000 Ghent, Belgium; Cancer Research Institute Ghent (CRIG), Ghent University, Ghent, Belgium., Regev A; Klarman Cell Observatory, Broad Institute of Harvard and MIT, Cambridge, MA, USA; Howard Hughes Medical Institute, Koch Institute of Integrative Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA., Van Vlierberghe P; Cancer Research Institute Ghent (CRIG), Ghent University, Ghent, Belgium; Department of Biomolecular Medicine, Ghent University, Ghent, Belgium., Guilliams M; Laboratory of Myeloid Cell Ontogeny and Functional Specialization, VIB Center for Inflammation Research, Ghent, Belgium; Faculty of Sciences, Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium., Teichmann SA; Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SA, UK; Theory of Condensed Matter Group, Cavendish Laboratory/Department of Physics, University of Cambridge, Cambridge CB3 0HE, UK., Saeys Y; Data Mining and Modeling for Biomedicine, VIB Center for Inflammation Research, Ghent, Belgium; Cancer Research Institute Ghent (CRIG), Ghent University, Ghent, Belgium., Taghon T; Faculty of Medicine and Health Sciences, Department of Diagnostic Sciences, Ghent University, C. Heymanslaan 10, MRB2, Entrance 38, 9000 Ghent, Belgium; Cancer Research Institute Ghent (CRIG), Ghent University, Ghent, Belgium. Electronic address: tom.taghon@ugent.be. |
| المصدر: | Immunity [Immunity] 2020 Jun 16; Vol. 52 (6), pp. 1088-1104.e6. Date of Electronic Publication: 2020 Apr 17. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 9432918 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-4180 (Electronic) Linking ISSN: 10747613 NLM ISO Abbreviation: Immunity Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Cambridge, MA : Cell Press Original Publication: Cambridge, Mass. : Cell Press, c1994- |
| مواضيع طبية MeSH: | Cell Differentiation*/genetics , Cell Differentiation*/immunology , Gene Expression Regulation, Developmental*, Lymphoid Progenitor Cells/*cytology , Lymphoid Progenitor Cells/*metabolism , RNA, Small Cytoplasmic/*genetics , Thymocytes/*cytology , Thymocytes/*metabolism, Biomarkers ; Cell Lineage/genetics ; Gene Expression Profiling ; High-Throughput Nucleotide Sequencing ; Humans ; Immunophenotyping ; Single-Cell Analysis ; Thymocytes/immunology ; Transcriptome |
| مستخلص: | During postnatal life, thymopoiesis depends on the continuous colonization of the thymus by bone-marrow-derived hematopoietic progenitors that migrate through the bloodstream. The current understanding of the nature of thymic immigrants is largely based on data from pre-clinical models. Here, we employed single-cell RNA sequencing (scRNA-seq) to examine the immature postnatal thymocyte population in humans. Integration of bone marrow and peripheral blood precursor datasets identified two putative thymus seeding progenitors that varied in expression of CD7; CD10; and the homing receptors CCR7, CCR9, and ITGB7. Whereas both precursors supported T cell development, only one contributed to intrathymic dendritic cell (DC) differentiation, predominantly of plasmacytoid dendritic cells. Trajectory inference delineated the transcriptional dynamics underlying early human T lineage development, enabling prediction of transcription factor (TF) modules that drive stage-specific steps of human T cell development. This comprehensive dataset defines the expression signature of immature human thymocytes and provides a resource for the further study of human thymopoiesis. Competing Interests: Declaration of Interests A.R. is an Scientific Advistory Board member of Thermo Fisher Scientific, Neogene Therapeutics, Asimov, and Syros Pharmaceuticals; an equity holder of Immunitas; and a founder of and equity holder in Celsius Therapeutics. The remaining authors declare no competing interests. (Copyright © 2020 Elsevier Inc. All rights reserved.) |
| التعليقات: | Comment in: Nat Immunol. 2023 Mar;24(3):389-390. (PMID: 36737626) |
| معلومات مُعتمدة: | WT206194 United Kingdom WT_ Wellcome Trust |
| فهرسة مساهمة: | Keywords: RNA sequencing; homing; human; lineage differentiation; single cell; thymus; transcriptomics |
| المشرفين على المادة: | 0 (Biomarkers) 0 (RNA, Small Cytoplasmic) |
| تواريخ الأحداث: | Date Created: 20200419 Date Completed: 20210322 Latest Revision: 20230416 |
| رمز التحديث: | 20230416 |
| DOI: | 10.1016/j.immuni.2020.03.019 |
| PMID: | 32304633 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1097-4180 |
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| DOI: | 10.1016/j.immuni.2020.03.019 |