دورية أكاديمية
A Bioguided Approach for the Screening of Antibacterial Compounds Isolated From the Hydroalcoholic Extract of the Native Brazilian Bee's Propolis Using Mollicutes as a Model.
| العنوان: | A Bioguided Approach for the Screening of Antibacterial Compounds Isolated From the Hydroalcoholic Extract of the Native Brazilian Bee's Propolis Using Mollicutes as a Model. |
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| المؤلفون: | Hochheim S; Graduate Chemistry Program, Universidade de Blumenau - FURB, Blumenau, Brazil., Pacassa Borges P; Biomedicine School, University of Blumenau - FURB, Blumenau, Brazil., Boeder AM; Pharmacy School, University of Blumenau - FURB, Blumenau, Brazil., Scharf DR; Department of Chemistry, University de Blumenau - FURB, Blumenau, Brazil., Simionatto EL; Graduate Chemistry Program, Universidade de Blumenau - FURB, Blumenau, Brazil.; Department of Chemistry, University de Blumenau - FURB, Blumenau, Brazil., Yamanaka CN; Department of Pharmaceutical Sciences, University of Blumenau - FURB, Blumenau, Brazil., Alberton MD; Graduate Chemistry Program, Universidade de Blumenau - FURB, Blumenau, Brazil.; Department of Pharmaceutical Sciences, University of Blumenau - FURB, Blumenau, Brazil., Guedes A; Department of Pharmaceutical Sciences, University of Blumenau - FURB, Blumenau, Brazil., de Cordova CMM; Graduate Chemistry Program, Universidade de Blumenau - FURB, Blumenau, Brazil.; Department of Pharmaceutical Sciences, University of Blumenau - FURB, Blumenau, Brazil. |
| المصدر: | Frontiers in microbiology [Front Microbiol] 2020 Apr 07; Vol. 11, pp. 558. Date of Electronic Publication: 2020 Apr 07 (Print Publication: 2020). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101548977 Publication Model: eCollection Cited Medium: Print ISSN: 1664-302X (Print) Linking ISSN: 1664302X NLM ISO Abbreviation: Front Microbiol Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne : Frontiers Research Foundation |
| مستخلص: | Nature is a vast source of medicinal substances, including propolis, which has been extensively investigated. Propolis is a resinous substance produced by bees from the exudates of plants that they collect and modify in their jaws; it is a rich and complex matrix with secondary metabolites of diverse botanical origins. The objective of this study was to apply an in vitro bioguided approach using as a model system the mollicutes with a sample of propolis from the Brazilian native bee Melipona quadrifasciata (mandaçaia) in order to identify potential new molecules with antimicrobial activity. A crude hydroalcoholic extract was obtained and submitted to liquid-liquid partitioning with solvents of different polarities, generating four different fractions: aqueous, dichloromethane, butanol, and ethyl acetate fractions. The antimollicute activity assays served as a basis for the bioguided fractionation. The dichloromethane fraction was the most promising, exhibiting a minimal inhibitory concentration (MIC) of 125 μg/mL against Mycoplasma pneumoniae . After purification by column liquid chromatography, a subfraction presenting MIC of 15.6 μg/mL against Mycoplasma genitalium was highlighted. The fractions were also tested against Escherichia coli , Staphylococcus aureus , and Pseudomonas aeruginosa . Using gas chromatography coupled to a mass spectrometer (GC-MS), several volatile compounds were identified in the non-polar fractions of this propolis. However, the more purified molecules had no better antimollicute activity than their original subfraction. Apparently, the synergism among its compounds is largely responsible for the antibacterial activity of the propolis of this native Brazilian bee. (Copyright © 2020 Hochheim, Pacassa Borges, Boeder, Scharf, Simionatto, Yamanaka, Alberton, Guedes and de Cordova.) |
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| فهرسة مساهمة: | Keywords: Brazilian native bees; antimicrobials; bioguided fractioning; mollicutes; mycoplasmas; propolis |
| تواريخ الأحداث: | Date Created: 20200423 Latest Revision: 20200928 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC7154171 |
| DOI: | 10.3389/fmicb.2020.00558 |
| PMID: | 32318040 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1664-302X |
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| DOI: | 10.3389/fmicb.2020.00558 |