دورية أكاديمية
Post-translational protein deimination signatures and extracellular vesicles (EVs) in the Atlantic horseshoe crab (Limulus polyphemus).
| العنوان: | Post-translational protein deimination signatures and extracellular vesicles (EVs) in the Atlantic horseshoe crab (Limulus polyphemus). |
|---|---|
| المؤلفون: | Bowden TJ; Aquaculture Research Institute, School of Food & Agriculture, University of Maine, University of Maine, Orono, ME, USA. Electronic address: timothy.bowden@maine.edu., Kraev I; Electron Microscopy Suite, Faculty of Science Technology, Engineering and Mathematics Open University, Milton Keynes, MK7 6AA, UK. Electronic address: igor.kraev@open.ac.uk., Lange S; Tissue Architecture and Regeneration Research Group, School of Life Sciences, University of Westminster, London, W1W 6UW, UK. Electronic address: S.Lange@westminster.ac.uk. |
| المصدر: | Developmental and comparative immunology [Dev Comp Immunol] 2020 Sep; Vol. 110, pp. 103714. Date of Electronic Publication: 2020 Apr 23. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: United States NLM ID: 7708205 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0089 (Electronic) Linking ISSN: 0145305X NLM ISO Abbreviation: Dev Comp Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Tarrytown Ny : Elsevier Science Original Publication: Elmsford, N. Y., Pergamon Press., 1977-2004. |
| مواضيع طبية MeSH: | Arthropod Proteins/*metabolism , Complement System Proteins/*metabolism , Extracellular Vesicles/*metabolism , Horseshoe Crabs/*metabolism , Protein-Arginine Deiminases/*metabolism , Staphylococcal Infections/*metabolism , Staphylococcus aureus/*physiology, Animals ; Arthropod Proteins/genetics ; Biological Evolution ; Blood Coagulation ; Cell Communication ; Citrullination ; Horseshoe Crabs/immunology ; Immunity, Innate ; Organelle Size ; Phylogeny ; Protein-Arginine Deiminases/genetics ; Staphylococcal Infections/immunology |
| مستخلص: | The horseshoe crab is a living fossil and a species of marine arthropod with unusual immune system properties which are also exploited commercially. Given its ancient status dating to the Ordovician period (450 million years ago), its standing in phylogeny and unusual immunological characteristics, the horseshoe crab may hold valuable information for comparative immunology studies. Peptidylarginine deiminases (PADs) are calcium dependent enzymes that are phylogenetically conserved and cause protein deimination via conversion of arginine to citrulline. This post-translational modification can lead to structural and functional protein changes contributing to protein moonlighting in health and disease. PAD-mediated regulation of extracellular vesicle (EV) release, a critical component of cellular communication, has furthermore been identified to be a phylogenetically conserved mechanism. PADs, protein deimination and EVs have hitherto not been studied in the horseshoe crab and were assessed in the current study. Horseshoe crab haemolymph serum-EVs were found to be a poly-dispersed population in the 20-400 nm size range, with the majority of EVs falling within 40-123 nm. Key immune proteins were identified to be post-translationally deiminated in horseshoe crab haemolymph serum, providing insights into protein moonlighting function of Limulus and phylogenetically conserved immune proteins. KEGG (Kyoto encyclopaedia of genes and genomes) and GO (gene ontology) enrichment analysis of deiminated proteins identified in Limulus revealed KEGG pathways relating to complement and coagulation pathways, Staphylococcus aureus infection, glycolysis/gluconeogenesis and carbon metabolism, while GO pathways of biological and molecular pathways related to a range of immune and metabolic functions, as well as developmental processes. The characterisation of EVs, and post-translational deimination signatures, revealed here in horseshoe crab, contributes to current understanding of protein moonlighting functions and EV-mediated communication in this ancient arthropod and throughout phylogeny. Competing Interests: Declaration of competing interest The authors declare no conflicting interest. (Copyright © 2020 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: CRP; Complement; Extracellular vesicles (EVs); Horseshoe crab (Limulus polyphemus); Innate immunity; Peptidylarginine deiminases (PADs); Protein deimination |
| المشرفين على المادة: | 0 (Arthropod Proteins) 9007-36-7 (Complement System Proteins) EC 3.5.3.15 (Protein-Arginine Deiminases) |
| تواريخ الأحداث: | Date Created: 20200427 Date Completed: 20210915 Latest Revision: 20210915 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.dci.2020.103714 |
| PMID: | 32335073 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0089 |
|---|---|
| DOI: | 10.1016/j.dci.2020.103714 |