التفاصيل البيبلوغرافية
| العنوان: |
Centrifugal Generation of Droplet-Based 3D Cell Cultures. |
| المؤلفون: |
De Lora JA; Center for Biomedical Engineering, University of New Mexico, Albuquerque, NM, USA.; Department of Chemical and Biological Engineering, University of New Mexico, Albuquerque, NM, USA., Velasquez JL; Center for Biomedical Engineering, University of New Mexico, Albuquerque, NM, USA., Carroll NJ; Center for Biomedical Engineering, University of New Mexico, Albuquerque, NM, USA.; Department of Chemical and Biological Engineering, University of New Mexico, Albuquerque, NM, USA., Freyer JP; Center for Biomedical Engineering, University of New Mexico, Albuquerque, NM, USA., Shreve AP; Center for Biomedical Engineering, University of New Mexico, Albuquerque, NM, USA.; Department of Chemical and Biological Engineering, University of New Mexico, Albuquerque, NM, USA. |
| المصدر: |
SLAS technology [SLAS Technol] 2020 Oct; Vol. 25 (5), pp. 436-445. Date of Electronic Publication: 2020 Apr 30. |
| نوع المنشور: |
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: |
English |
| بيانات الدورية: |
Publisher: SAGE Publications Country of Publication: United States NLM ID: 101697564 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2472-6311 (Electronic) Linking ISSN: 24726303 NLM ISO Abbreviation: SLAS Technol Subsets: MEDLINE |
| أسماء مطبوعة: |
Original Publication: Thousand Oaks, CA : SAGE Publications, [2017]- |
| مواضيع طبية MeSH: |
Imaging, Three-Dimensional*, Cell Culture Techniques/*methods, Calibration ; Cell Culture Techniques/instrumentation ; Cell Line, Tumor ; Cells, Immobilized/cytology ; Centrifugation ; Humans ; Suspensions |
| مستخلص: |
Quickly and easily producing uniform populations of microsphere-based 3D cell cultures using droplet-based templating methods has the potential to enable widespread use of such platforms in drug discovery or cancer research. Here, we advance the design of centrifuge-based droplet generation devices, describe the use of this platform for droplet generation with controlled cell occupancy, and demonstrate weeklong culture duration. Using simple-to-construct devices and easily implemented protocols, the initial concentration of encapsulated cells is adjustable up to hundreds of cells per microsphere. This work demonstrates the first instance of using centrifugal droplet-generating devices to produce large numbers of cell-encapsulating microspheres. Applications of this versatile methodology include the rapid formation of templated 3D cell culture populations suitable for suspension culture or large batch bioreactor studies that require uniform populations. |
| معلومات مُعتمدة: |
F31 CA189682 United States CA NCI NIH HHS |
| فهرسة مساهمة: |
Keywords: 3D cell culture; alginate; cell encapsulation; centrifugal droplet generation; spheroids |
| المشرفين على المادة: |
0 (Suspensions) |
| تواريخ الأحداث: |
Date Created: 20200501 Date Completed: 20210729 Latest Revision: 20210729 |
| رمز التحديث: |
20240628 |
| DOI: |
10.1177/2472630320915837 |
| PMID: |
32351161 |
| قاعدة البيانات: |
MEDLINE |
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