دورية أكاديمية
Latency characteristics in specific pathogen-free chickens 21 and 35 days after intra-tracheal inoculation with vaccine or field strains of infectious laryngotracheitis virus.
| العنوان: | Latency characteristics in specific pathogen-free chickens 21 and 35 days after intra-tracheal inoculation with vaccine or field strains of infectious laryngotracheitis virus. |
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| المؤلفون: | Thilakarathne DS; Asia-Pacific Centre for Animal Health, Melbourne Veterinary School, The University of Melbourne, Parkville, Australia., Hartley CA; Asia-Pacific Centre for Animal Health, Melbourne Veterinary School, The University of Melbourne, Parkville, Australia., Diaz-Méndez A; Asia-Pacific Centre for Animal Health, Melbourne Veterinary School, The University of Melbourne, Parkville, Australia., Quinteros JA; Asia-Pacific Centre for Animal Health, Melbourne Veterinary School, The University of Melbourne, Parkville, Australia., Fakhri O; Asia-Pacific Centre for Animal Health, Melbourne Veterinary School, The University of Melbourne, Parkville, Australia., Coppo MJC; Asia-Pacific Centre for Animal Health, Melbourne Veterinary School, The University of Melbourne, Parkville, Australia., Devlin JM; Asia-Pacific Centre for Animal Health, Melbourne Veterinary School, The University of Melbourne, Parkville, Australia. |
| المصدر: | Avian pathology : journal of the W.V.P.A [Avian Pathol] 2020 Aug; Vol. 49 (4), pp. 369-379. Date of Electronic Publication: 2020 Jun 02. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Informa Healthcare Country of Publication: England NLM ID: 8210638 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1465-3338 (Electronic) Linking ISSN: 03079457 NLM ISO Abbreviation: Avian Pathol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London : Informa Healthcare Original Publication: Houghton, Huntingdon, Cambs. : World Veterinary Poultry Association. |
| مواضيع طبية MeSH: | Chickens/*virology , Herpesviridae Infections/*veterinary , Herpesvirus 1, Gallid/*immunology , Poultry Diseases/*virology, Animals ; Herpesviridae Infections/virology ; Herpesvirus 1, Gallid/physiology ; Leukocytes, Mononuclear/immunology ; Male ; Polymerase Chain Reaction/veterinary ; Specific Pathogen-Free Organisms ; Trachea/virology ; Trigeminal Ganglion/virology ; Virus Latency |
| مستخلص: | Latency is an important feature of infectious laryngotracheitis virus (ILTV) yet is poorly understood. This study aimed to compare latency characteristics of vaccine (SA2) and field (CL9) strains of ILTV, establish an in vitro reactivation system and examine ILTV infection in peripheral blood mononuclear cells (PBMC) in specific pathogen-free chickens. Birds were inoculated with SA2 or CL9 ILTV and then bled and culled at 21 or 35 days post-inoculation (dpi). Swabs (conjunctiva, palatine cleft, trachea) and trigeminal ganglia (TG) were examined for ILTV DNA using PCR. Half of the TG, trachea and PBMC were co-cultivated with cell monolayers to assess in vitro reactivation of ILTV infection. ILTV DNA was detected in the trachea of approximately 50% of ILTV-inoculated birds at both timepoints. At 21 dpi, ILTV was detected in the TG only in 29% and 17% of CL9- and SA2-infected birds, respectively. At 35 dpi, ILTV was detected in the TG only in 30% and 10% of CL9- and SA2-infected birds, respectively. Tracheal organ co-cultures from 30% and 70% of CL9- and SA2-infected birds, respectively, were negative for ILTV DNA at cull but yielded quantifiable DNA within 6 days post-explant (dpe). TG co-cultivation from 30% and 40% of CL9-and SA2-infected birds, respectively, had detectable ILTV DNA within 6 dpe. Latency characteristics did not substantially vary based on the strain of virus inoculated or between sampling timepoints. These results advance our understanding of ILTV latency and reactivation. RESEARCH HIGHLIGHTS Following inoculation, latent ILTV infection was detected in a large proportion of chickens, irrespective of whether a field or vaccine strain was inoculated. In vitro reactivation of latent ILTV was readily detected in tracheal and trigeminal ganglia co-cultures using PCR. ILTV latency observed in SPF chickens at 21 days post-infection was not substantially different to 35 days post-infection. |
| فهرسة مساهمة: | Keywords: in vitro reactivation; Chickens; infectious laryngotracheitis virus; latency |
| تواريخ الأحداث: | Date Created: 20200501 Date Completed: 20210315 Latest Revision: 20210315 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1080/03079457.2020.1754331 |
| PMID: | 32352307 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1465-3338 |
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| DOI: | 10.1080/03079457.2020.1754331 |