دورية أكاديمية
Antibacterial mode of action of trans-cinnamaldehyde derived from cinnamon bark (Cinnamomum verum) essential oil against Agrobacterium tumefaciens.
| العنوان: | Antibacterial mode of action of trans-cinnamaldehyde derived from cinnamon bark (Cinnamomum verum) essential oil against Agrobacterium tumefaciens. |
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| المؤلفون: | Lee JE; Department of Forest Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea; Forest Insect Pests and Diseases Division, National Institute of Forest Science, Seoul 02455, Republic of Korea., Jung M; Department of Forest Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea; Bioinformatics Group, R&D Center, Insilicogen, Inc., Yongin-si, Gyeonggi-do 16954, Republic of Korea., Lee SC; Department of Forest Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea., Huh MJ; Department of Forest Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea., Seo SM; Department of Forest Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea; Research Institute of Agriculture and Life Science, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea., Park IK; Department of Forest Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea; Research Institute of Agriculture and Life Science, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea. Electronic address: parkik1@snu.ac.kr. |
| المصدر: | Pesticide biochemistry and physiology [Pestic Biochem Physiol] 2020 May; Vol. 165, pp. 104546. Date of Electronic Publication: 2020 Feb 19. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 1301573 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9939 (Electronic) Linking ISSN: 00483575 NLM ISO Abbreviation: Pestic Biochem Physiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York Ny : Academic Press |
| مواضيع طبية MeSH: | Cinnamomum zeylanicum* , Oils, Volatile*, Acrolein/analogs & derivatives ; Agrobacterium tumefaciens ; Anti-Bacterial Agents ; Plant Bark |
| مستخلص: | The fumigant antibacterial activities of 50 plant essential oils belonging to 10 families were investigated against Agrobacterium tumefaciens. Among the test plant essential oils, Cinnamomum verum (cinnamon bark) essential oil showed the most potent fumigant antibacterial activity. When we investigated the antibacterial activities of compounds identified from cinnamon bark essential oil and 9 congeners of trans-cinnamaldehyde, lengths of inhibition zone of trans-cinnamaldehyde, salicylaldehyde and hydrocinnamaldehyde were 1.28, 1.73, and 1.24 cm at 0.625 mg/paper disc concentration, respectively. To determine the mode of action of trans-cinnamaldehyde and salicylaldehyde, intercellular reactive oxygen species (ROS) generation and cell membrane integrity were determined using a confocal laser scanning microscopy. Furthermore, we compared the up- and down-regulated gene expression of A. tumefaciens treated with trans-cinnamaldehyde and salicylaldehyde with that of untreated A. tumefaciens. With cutoffs of |log2FC| > 1 and FDR < 0.05, 29 and 43 down-regulated genes and 27 and 117 up-regulated genes were found in the treatment of trans-cinnamaldehyde and salicylaldehyde, respectively. Based on the ROS generation results, cell membrane integrity assay, and gene expression, we conclude that the antibacterial mode of action of trans-cinnamaldehyde and salicylaldehyde is ROS generation by the Fenton reaction caused by the down-regulation of an ATP synthesis-related gene cluster, corrupted iron ion homeostasis, and a corrupted ROS defense mechanism. The high concentration of ROS damaged the A. tumefaciens cell membrane, which caused cell death. (Copyright © 2020. Published by Elsevier Inc.) |
| فهرسة مساهمة: | Keywords: Antibacterial mode of action; Fenton reaction; Reactive oxygen species; Salicyaldehyde; trans-Cinnamaldehyde |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) 0 (Oils, Volatile) 7864XYD3JJ (Acrolein) SR60A3XG0F (cinnamaldehyde) |
| تواريخ الأحداث: | Date Created: 20200504 Date Completed: 20200505 Latest Revision: 20210702 |
| رمز التحديث: | 20240829 |
| DOI: | 10.1016/j.pestbp.2020.02.012 |
| PMID: | 32359541 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1095-9939 |
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| DOI: | 10.1016/j.pestbp.2020.02.012 |