دورية أكاديمية
Testicular hyperthermia reduces testosterone concentrations and alters gene expression in testes of Nelore bulls.
| العنوان: | Testicular hyperthermia reduces testosterone concentrations and alters gene expression in testes of Nelore bulls. |
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| المؤلفون: | Rizzoto G; Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada., Ferreira JCP; Department of Veterinary Surgery and Animal Reproduction, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu, SP, Brazil. Electronic address: joao.cp.ferreira@unesp.br., Codognoto VM; Department of Veterinary Surgery and Animal Reproduction, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu, SP, Brazil., Oliveira KC; Department of Veterinary Clinical Science, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu, SP, Brazil., Mogollón García HD; Department of Veterinary Surgery and Animal Reproduction, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu, SP, Brazil., Pupulim AGR; Department of Veterinary Surgery and Animal Reproduction, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu, SP, Brazil., Teixeira-Neto FJ; Department of Veterinary Surgery and Animal Reproduction, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu, SP, Brazil., Castilho A; Western São Paulo University (Unoeste), Presidente Prudente, São Paulo, Brazil., Nunes SG; Department of Pharmacology, Institute of Biosciences, São Paulo State University, (UNESP), Botucatu, SP, Brazil., Thundathil JC; Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada., Kastelic JP; Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada. |
| المصدر: | Theriogenology [Theriogenology] 2020 Aug; Vol. 152, pp. 64-68. Date of Electronic Publication: 2020 Apr 23. |
| نوع المنشور: | Journal Article; Randomized Controlled Trial, Veterinary |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0421510 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3231 (Electronic) Linking ISSN: 0093691X NLM ISO Abbreviation: Theriogenology Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: [New York, N.Y.?] : Elsevier Original Publication: Los Altos, Calif., Geron-X. |
| مواضيع طبية MeSH: | Gene Expression Regulation/*physiology , Hot Temperature/*adverse effects , Testis/*physiology , Testosterone/*metabolism, Animals ; Antioxidants/metabolism ; Cattle ; Male ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Stress, Physiological |
| مستخلص: | Increased testicular temperature reduces sperm motility, morphology and fertility. Our objectives were to characterize effects of testicular hyperthermia (scrotal insulation) on acute testosterone concentrations and gene expression in Bos indicus testes. Nelore bulls (n = 20), ∼27 mo of age, 375 kg, scrotal circumference >31 cm, with ≥30% motile sperm, were allocated into four groups (n = 5/group): non-insulated (Control) and insulation removed after 12, 24, or 48 h. Immediately after insulation, intratesticular temperatures (needle thermocouples) were coolest in Control bulls and warmest in 48-h bulls (mean ± SEM, 35.28 ± 0.31 vs 38.62 ± 0.57 °C, P < 0.05). Bulls were castrated and testes recovered. Testicular testosterone concentrations were higher in Control versus 48-h bulls (3119 ± 973.3 and 295.5 ± 122.8 ng/g of tissue, respectively, P < 0.05). Total RNA was extracted, reverse transcribed and RT-qPCR done. For STAR, mRNA abundance decreased from Control to 48 h (1.14 + 0.32 vs 0.32 + 0.5, P < 0.05). For BCL2, expression decreased from Control to 24 h (1.00 + 0.07 vs 0.70 + 0.12, P < 0.05), but then rebounded. In addition, GPX1 had a 70% increase (P < 0.05) at 48 h, whereas HSP70 had a 34-fold increase (P < 0.05) at 12 h and 2- and 14-fold increases (P < 0.05) at 24 and 48 h, respectively. HSF1, BAX, P53 and CASP 8 remained unchanged. Downregulation of STAR, critical in androgen production, was consistent with reduced testosterone concentrations, whereas increased GPX1 enhanced testicular antioxidative capability. Huge increases in HSP70 conferred protection again apoptosis and cell destruction, whereas reduced BCL2 promoted apoptosis. These findings provided novel insights into acute tissue responses (testosterone and gene activity) to testicular hyperthermia in B. indicus bulls. Competing Interests: Declaration of competing interest The authors declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research reported. (Copyright © 2020. Published by Elsevier Inc.) |
| فهرسة مساهمة: | Keywords: BCL2; GPX1; HSP70; STAR; Scrotal/testicular thermoregulation; Testicular hyperthermia |
| المشرفين على المادة: | 0 (Antioxidants) 0 (RNA, Messenger) 3XMK78S47O (Testosterone) |
| تواريخ الأحداث: | Date Created: 20200508 Date Completed: 20210329 Latest Revision: 20210329 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.theriogenology.2020.04.029 |
| PMID: | 32380276 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-3231 |
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| DOI: | 10.1016/j.theriogenology.2020.04.029 |