دورية أكاديمية
أعرض في EDS

Molecular epidemiology, genetic diversity and antimicrobial resistance of Staphylococcus aureus isolated from chicken and pig carcasses, and carcass handlers.

التفاصيل البيبلوغرافية
العنوان: Molecular epidemiology, genetic diversity and antimicrobial resistance of Staphylococcus aureus isolated from chicken and pig carcasses, and carcass handlers.
المؤلفون: Okorie-Kanu OJ; Department of Veterinary Public Health and Preventive Medicine, University of Nigeria, Nsukka, Enugu State, Nigeria., Anyanwu MU; Microbiology Unit, Department of Veterinary Microbiology and Pathology, University of Nigeria, Nsukka, Enugu State, Nigeria., Ezenduka EV; Department of Veterinary Public Health and Preventive Medicine, University of Nigeria, Nsukka, Enugu State, Nigeria., Mgbeahuruike AC; Microbiology Unit, Department of Veterinary Microbiology and Pathology, University of Nigeria, Nsukka, Enugu State, Nigeria., Thapaliya D; Department of Biostatistics, Environmental Health Sciences and Epidemiology, Kent State University, Kent, Ohio, United States of America., Gerbig G; Department of Biostatistics, Environmental Health Sciences and Epidemiology, Kent State University, Kent, Ohio, United States of America., Ugwuijem EE; Department of Microbiology, University of Nigeria, Nsukka, Enugu State, Nigeria., Okorie-Kanu CO; Department of Veterinary Pathology, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria., Agbowo P; Department of Veterinary Public Health and Preventive Medicine, University of Nigeria, Nsukka, Enugu State, Nigeria., Olorunleke S; Department of Veterinary Public Health and Preventive Medicine, University of Nigeria, Nsukka, Enugu State, Nigeria., Nwanta JA; Department of Veterinary Public Health and Preventive Medicine, University of Nigeria, Nsukka, Enugu State, Nigeria., Chah KF; Microbiology Unit, Department of Veterinary Microbiology and Pathology, University of Nigeria, Nsukka, Enugu State, Nigeria., Smith TC; Department of Biostatistics, Environmental Health Sciences and Epidemiology, Kent State University, Kent, Ohio, United States of America.
المصدر: PloS one [PLoS One] 2020 May 14; Vol. 15 (5), pp. e0232913. Date of Electronic Publication: 2020 May 14 (Print Publication: 2020).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Genetic Variation* , Molecular Epidemiology*, Anti-Bacterial Agents/*pharmacology , Bacterial Proteins/*genetics , Drug Resistance, Bacterial/*genetics , Staphylococcal Infections/*microbiology , Staphylococcus aureus/*classification, Abattoirs ; Animals ; Chickens ; Staphylococcal Infections/epidemiology ; Staphylococcus aureus/drug effects ; Staphylococcus aureus/genetics ; Staphylococcus aureus/isolation & purification ; Swine
مستخلص: The epidemiology of Staphylococcus aureus in food animals, associated products, and their zoonotic potential in Nigeria are poorly understood. This study aimed to provide data on the prevalence, genetic characteristics and antimicrobial resistance of S. aureus isolated from chicken and pig carcasses, and persons in contact with the carcasses at slaughterhouses in Nigeria. Surface swabs were collected randomly from 600 chicken and 600 pig carcasses. Nasal swabs were collected from 45 workers in chicken slaughterhouses and 45 pig slaughterhouse workers. S. aureus isolates were analyzed by spa typing. They were also examined for presence of the Panton-Valentine Leucocidin (PVL) and mecA genes, as well as for antimicrobial resistance phenotype. Overall, 53 S. aureus isolates were recovered (28 from chicken carcasses, 17 from pig carcasses, 5 from chicken carcass handlers and 3 from pig carcass handlers). Among the isolates, 19 (35.8%) were PVL-positive and 12 (22.6%) carried the mecA gene. The 53 isolates belonged to 19 spa types. The Based Upon Repeat Pattern (BURP) algorithm separated the isolates into 2 spa-clonal complexes (spa-CC) and 9 singletons including 2 novel spa types (t18345 and t18346). The clonal complexes (CC) detected were CC1, CC5, CC8, CC15, CC88 and CC152. CC15-related isolates represented by spa type t084 (32.1%) and CC5 represented by spa type t311 (35.3%) predominated among isolates from chicken carcasses/ handlers, and pig carcasses/ handlers, respectively. Multidrug resistance exhibited by all the CC except CC8, was observed among isolates from chicken carcasses (64.3%), pig carcasses (41.2%), handlers of chicken meat (40.0%) and handlers of pork (33.3%). All the CC showed varying degrees of resistance to tetracycline while CC15 and CC5 exhibited the highest resistance to sulphamethoxazole/trimethoprim and erythromycin, respectively. The predominant antimicrobial resistance pattern observed was penicillin-tetracycline-sulphamethoxazole/trimethoprim (PEN-TET-SXT). In conclusion, food animals processed in Enugu State in Southeast Nigeria are potential vehicles for transmission of PVL-positive multiple-drug resistant S. aureus and methicillin-resistant S. aureus from farm to slaughterhouse and potentially to the human population. Public health intervention programs at pre- and post-slaughter stages should be considered in Nigerian slaughterhouses.
Competing Interests: The authors have declared that no competing interests exist.
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المشرفين على المادة: 0 (Anti-Bacterial Agents)
0 (Bacterial Proteins)
تواريخ الأحداث: Date Created: 20200515 Date Completed: 20200804 Latest Revision: 20200804
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC7224487
DOI: 10.1371/journal.pone.0232913
PMID: 32407414
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0232913