دورية أكاديمية
Histochemical Staining and Quantification of Arbuscular Mycorrhizal Fungal Colonization.
| العنوان: | Histochemical Staining and Quantification of Arbuscular Mycorrhizal Fungal Colonization. |
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| المؤلفون: | Ho-Plágaro T; Department of Soil Microbiology and Symbiotic Systems, Estación Experimental del Zaidín (EEZ) CSIC, Granada, Spain., Tamayo-Navarrete MI; Department of Soil Microbiology and Symbiotic Systems, Estación Experimental del Zaidín (EEZ) CSIC, Granada, Spain., García-Garrido JM; Department of Soil Microbiology and Symbiotic Systems, Estación Experimental del Zaidín (EEZ) CSIC, Granada, Spain. josemanuel.garcia@eez.csic.es. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2020; Vol. 2146, pp. 43-52. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Mycorrhizae/*isolation & purification , Plant Roots/*microbiology , Staining and Labeling/*methods, Mycorrhizae/cytology ; Mycorrhizae/ultrastructure ; Phosphorus/metabolism ; Plant Roots/ultrastructure ; Soil Microbiology |
| مستخلص: | Histochemical staining and light microscopy-based techniques have been widely used to detect and quantify arbuscular mycorrhizal fungi (AMF) in roots. Here we describe a standardized method for staining of AMF in colonized roots, and we provide possible modifications to adjust the protocol according to particular requirements, such as the type of root material or the reduction of toxic products. In addition, we also summarize some of the most common ways to quantify arbuscular mycorrhizal colonization. |
| فهرسة مساهمة: | Keywords: AM quantification; Arbuscular mycorrhizal roots; Non-vital staining; Trypan blue |
| المشرفين على المادة: | 27YLU75U4W (Phosphorus) |
| تواريخ الأحداث: | Date Created: 20200517 Date Completed: 20210315 Latest Revision: 20210315 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1007/978-1-0716-0603-2_4 |
| PMID: | 32415594 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-6029 |
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| DOI: | 10.1007/978-1-0716-0603-2_4 |