دورية أكاديمية
FOXD3 inhibits cell proliferation, migration, and invasion in nasopharyngeal carcinoma through regulation of the PI3K-Akt pathway.
| العنوان: | FOXD3 inhibits cell proliferation, migration, and invasion in nasopharyngeal carcinoma through regulation of the PI3K-Akt pathway. |
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| المؤلفون: | Xie X; Department of Otolaryngology, Tongde Hospital of Zhejiang Province, Hangzhou, Zhejiang 310012, P.R. China., Xiong G; Department of Otolaryngology, Tongde Hospital of Zhejiang Province, Hangzhou, Zhejiang 310012, P.R. China., Chen W; Department of Otolaryngology, The Traditional Chinese Medicine Hospital of Haiyan County, Jiaxing, Zhejiang 314300, P.R. China., Fu H; Department of Otolaryngology, The Traditional Chinese Medicine Hospital of Haiyan County, Jiaxing, Zhejiang 314300, P.R. China., Li M; Cancer Institute of Integrated Traditional Chinese and Western Medicine, Zhejiang Academy of Traditional Chinese Medicine, Tongde Hospital of Zhejiang Province, Hangzhou, Zhejiang 310012, P.R. China., Cui X; Department of Anesthesiology, Zhejiang Cancer Hospital, Hangzhou, Zhejiang 310022, P.R. China. |
| المصدر: | Biochemistry and cell biology = Biochimie et biologie cellulaire [Biochem Cell Biol] 2020 Dec; Vol. 98 (6), pp. 653-660. Date of Electronic Publication: 2020 May 27. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Canadian Science Publishing Country of Publication: Canada NLM ID: 8606068 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1208-6002 (Electronic) Linking ISSN: 08298211 NLM ISO Abbreviation: Biochem Cell Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2011- : Ottawa, ON : Canadian Science Publishing Original Publication: Ottawa, Ont. : National Research Council of Canada, c1986- |
| مواضيع طبية MeSH: | Cell Movement* , Cell Proliferation* , Signal Transduction*, Forkhead Transcription Factors/*metabolism , Nasopharyngeal Carcinoma/*metabolism , Nasopharyngeal Neoplasms/*metabolism , Phosphatidylinositol 3-Kinases/*metabolism , Proto-Oncogene Proteins c-akt/*metabolism, Cell Line, Tumor ; Forkhead Transcription Factors/genetics ; Humans ; Nasopharyngeal Carcinoma/genetics ; Nasopharyngeal Carcinoma/pathology ; Nasopharyngeal Neoplasms/genetics ; Nasopharyngeal Neoplasms/pathology ; Neoplasm Invasiveness ; Phosphatidylinositol 3-Kinases/genetics ; Proto-Oncogene Proteins c-akt/genetics |
| مستخلص: | FOXD3 has been found previously to positively regulate miR-26b, a tumor inhibitor of nasopharyngeal carcinoma (NPC). However, FOXD3's precise function and associated mechanism of action in NPC have not yet been investigated. In this study, the expression of FOXD3 mRNA and protein was evaluated using RT-qPCR, western blotting, and immunohistochemistry. Protein levels involved in the phosphoinositide 3-kinase - protein kinase B (PI3K-Akt) pathway were assessed by western blot, and cell proliferation was determined by MTT and colony forming assays. Additionally, cell apoptosis was assessed by flow cytometric assay. Finally, the migration and invasion capabilities of the NPC cells were determined using wound healing and Transwell assays. We found that FOXD3 levels were relatively low in NPC tissue and cells, while an increase caused the inhibition of the PI3K-Akt pathway. Functional experiments found that overexpression of FOXD3 suppressed cell proliferation, migration, and invasion and enhanced cell apoptosis in NPC C6661 cells. IGF-1, an activator of the PI3K-Akt pathway, reversed the inhibitory effect of FOXD3. Furthermore, we found upregulation of the PI3K-Akt pathway and upregulation of the inhibitory effects of FOXD3 on C6661 cellular activities. In conclusion, FOXD3 negatively affected the PI3K-Akt pathway to restrain the processes involved in C6661 cell pathology. These findings further exposed the function and downstream axis of FOXD3 in NPC and displayed a promising new target for NPC therapy. |
| فهرسة مساهمة: | Keywords: FOXD3; PI3K–Akt pathway; carcinome nasopharyngien (CNP); nasopharyngeal carcinoma (NPC); voie PI3K–Akt |
| المشرفين على المادة: | 0 (FOXD3 protein, human) 0 (Forkhead Transcription Factors) EC 2.7.11.1 (Proto-Oncogene Proteins c-akt) |
| تواريخ الأحداث: | Date Created: 20200528 Date Completed: 20210610 Latest Revision: 20210610 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1139/bcb-2020-0011 |
| PMID: | 32459973 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1208-6002 |
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| DOI: | 10.1139/bcb-2020-0011 |