دورية أكاديمية
أعرض في EDS

Ubiquitome analysis reveals the involvement of lysine ubiquitination in the spermatogenesis process of adult buffalo (Bubalus bubalis) testis.

التفاصيل البيبلوغرافية
العنوان: Ubiquitome analysis reveals the involvement of lysine ubiquitination in the spermatogenesis process of adult buffalo (Bubalus bubalis) testis.
المؤلفون: Huang YL; College of Life Science and Technology, Guangxi University, Nanning, Guangxi, China.; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Animal Reproduction Institute, Guangxi University, Nanning, Guangxi, China.; Department of Cell and Genetics, College of Basic Medicine, Guangxi University of Chinese Medicine, Nanning 530004, Guangxi, China., Zhang PF; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Animal Reproduction Institute, Guangxi University, Nanning, Guangxi, China., Hou Z; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Animal Reproduction Institute, Guangxi University, Nanning, Guangxi, China., Fu Q; College of Life Science and Technology, Guangxi University, Nanning, Guangxi, China.; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Animal Reproduction Institute, Guangxi University, Nanning, Guangxi, China., Li MX; Department of Cell and Genetics, College of Basic Medicine, Guangxi University of Chinese Medicine, Nanning 530004, Guangxi, China., Huang DL; Department of Cell and Genetics, College of Basic Medicine, Guangxi University of Chinese Medicine, Nanning 530004, Guangxi, China., Deng TX; Key Laboratory of Buffalo Genetics, Breeding and Reproduction Technology, Ministry of Agriculture and Guangxi, Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning, Guangxi, China., Lu YQ; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Animal Reproduction Institute, Guangxi University, Nanning, Guangxi, China., Liang XW; Key Laboratory of Buffalo Genetics, Breeding and Reproduction Technology, Ministry of Agriculture and Guangxi, Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning, Guangxi, China., Zhang M; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Animal Reproduction Institute, Guangxi University, Nanning, Guangxi, China.
المصدر: Bioscience reports [Biosci Rep] 2020 Jun 26; Vol. 40 (6).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Portland Press on behalf of the Biochemical Society Country of Publication: England NLM ID: 8102797 Publication Model: Print Cited Medium: Internet ISSN: 1573-4935 (Electronic) Linking ISSN: 01448463 NLM ISO Abbreviation: Biosci Rep Subsets: MEDLINE
أسماء مطبوعة: Publication: London : Portland Press on behalf of the Biochemical Society
Original Publication: London : The Biochemical Society, c1981-
مواضيع طبية MeSH: Proteome* , Proteomics* , Spermatogenesis*, Buffaloes/*metabolism , Proteins/*metabolism , Testis/*metabolism, Amino Acid Motifs ; Animals ; Chromatography, Liquid ; Computational Biology ; Lysine ; Male ; Protein Interaction Maps ; Signal Transduction ; Spectrometry, Mass, Electrospray Ionization ; Tandem Mass Spectrometry ; Ubiquitination
مستخلص: Protein ubiquitination, a major and conserved post-translational modification, is known to play a critical regulatory role in many biological processes in eukaryotes. Although several ubiquitinated proteins have been found in buffalo (Bubalus bubalis) testis in our previous studies, large-scale profiling of buffalo testis ubiquitome has not been reported to date. In the present study, we first identified a global profiling of lysine ubiquitination of adult buffalo testis using a highly sensitive LC-MS/MS coupled with immune-affinity enrichment of ubiquitinated peptides. In total, 422 lysine ubiquitination sites were identified in 262 proteins in adult buffalo testis tissue. Bioinformatics analysis showed that the ubiquitinated proteins are involved in a variety of biological processes and diverse subcellular localizations. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and protein interaction network analysis indicated that proteasome, glycolysis/gluconeogenesis and gap junction pathways are modulated by protein ubiquitination in testis. Besides, 44 ubiquitinated proteins may involve in spermatogenesis according to the SpermatogenesisOnline database, of which, the ubiquitination of HSPA2 and UCHL1 were confirmed by Immunoprecipitation (IP)/Western blot analysis. Taken together, these data provide a global view of ubiquitome in buffalo testis for the first time, and serve as an important resource for exploring the physiological role especially spermatogenesis of lysine ubiquitination in testis in mammals.
(© 2020 The Author(s).)
References: J Biol Chem. 1997 Nov 7;272(45):28557-62. (PMID: 9353319)
Dev Biol. 2009 Jan 1;325(1):33-42. (PMID: 18926813)
Nucleic Acids Res. 2013 Jan;41(Database issue):D1055-62. (PMID: 23193286)
PLoS One. 2010 Oct 27;5(10):e13654. (PMID: 21048919)
Mol Biol Cell. 2014 Aug 1;25(15):2260-71. (PMID: 24899640)
Nat Biotechnol. 2003 Aug;21(8):921-6. (PMID: 12872131)
Mol Cell. 2015 Apr 2;58(1):8-20. (PMID: 25839432)
Curr Protoc Bioinformatics. 2011 Sep;Chapter 13:Unit 13.15-24. (PMID: 21901740)
Anim Reprod Sci. 2000 Jul 2;60-61:471-80. (PMID: 10844217)
Nucleic Acids Res. 2015 Jan;43(Database issue):D1057-63. (PMID: 25378336)
EMBO J. 2006 Dec 13;25(24):5742-53. (PMID: 17139257)
Genetics. 1999 Mar;151(3):1065-79. (PMID: 10049923)
J Proteome Res. 2008 Oct;7(10):4566-76. (PMID: 18781797)
Mol Cell Endocrinol. 2010 Oct 7;327(1-2):89-97. (PMID: 20558241)
Mol Cell. 2009 Feb 13;33(3):275-86. (PMID: 19217402)
Nucleic Acids Res. 2016 Jan 4;44(D1):D447-56. (PMID: 26527722)
Arch Histol Cytol. 1991 Dec;54(5):491-509. (PMID: 1793664)
PLoS One. 2015 Nov 10;10(11):e0140650. (PMID: 26556802)
PLoS One. 2014 Jan 17;9(1):e85744. (PMID: 24465676)
Biol Reprod. 2005 Jul;73(1):29-35. (PMID: 15744022)
Nat Biotechnol. 2010 Aug;28(8):868-73. (PMID: 20639865)
Nat Rev Mol Cell Biol. 2011 May;12(5):295-307. (PMID: 21448225)
Mol Cell Biol. 2006 Apr;26(8):2999-3007. (PMID: 16581775)
Mol Cell Proteomics. 2011 May;10(5):M110.002089. (PMID: 20972266)
Dev Dyn. 2009 Jul;238(7):1851-61. (PMID: 19517565)
Mol Reprod Dev. 2006 Jan;73(1):40-9. (PMID: 16177983)
Nature. 2009 Mar 26;458(7237):461-7. (PMID: 19325626)
J Biol Chem. 2006 Dec 8;281(49):37888-92. (PMID: 17035236)
Mol Biol Rep. 2013 Apr;40(4):3213-30. (PMID: 23268313)
EMBO J. 2002 Jul 1;21(13):3516-25. (PMID: 12093752)
Microsc Res Tech. 2003 May 1;61(1):88-102. (PMID: 12672125)
Nat Rev Mol Cell Biol. 2010 Jul;11(7):479-89. (PMID: 20551964)
Mol Cell Proteomics. 2012 Dec;11(12):1529-40. (PMID: 22729469)
Mol Cell Biol. 2005 Apr;25(7):2819-31. (PMID: 15767685)
Dev Biol. 2011 Apr 15;352(2):278-87. (PMID: 21291880)
Biol Reprod. 2004 Aug;71(2):515-21. (PMID: 15084487)
Dev Cell. 2007 Nov;13(5):705-16. (PMID: 17981138)
Proteomics. 2016 Jul;16(14):2005-18. (PMID: 27173832)
Mol Hum Reprod. 2016 Feb;22(2):93-109. (PMID: 26676989)
Reproduction. 2011 Jul;142(1):133-44. (PMID: 21487003)
Mol Cell. 2011 Oct 21;44(2):325-40. (PMID: 21906983)
J Biol Chem. 2014 Dec 12;289(50):34490-502. (PMID: 25320092)
Nature. 2009 Oct 22;461(7267):1071-8. (PMID: 19847258)
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. (PMID: 22790023)
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. (PMID: 21890473)
Dev Dyn. 2007 Oct;236(10):2889-98. (PMID: 17823942)
Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. (PMID: 21139048)
PLoS One. 2010 Dec 31;5(12):e15770. (PMID: 21209834)
Bioinformatics. 2012 Jul 1;28(13):1805-6. (PMID: 22543366)
Curr Opin Cell Biol. 1995 Apr;7(2):215-23. (PMID: 7612274)
Cells. 2013 Dec 05;2(4):732-50. (PMID: 24709878)
Nucleic Acids Res. 2015 Jan;43(Database issue):D213-21. (PMID: 25428371)
Microbiol Immunol. 2012 Mar;56(3):163-70. (PMID: 22308980)
J Proteome Res. 2017 May 5;16(5):1988-2003. (PMID: 28349685)
BMC Struct Biol. 2009 Jul 31;9:51. (PMID: 19646261)
Int Immunopharmacol. 2013 Jul;16(3):348-52. (PMID: 23415874)
Plant Cell. 2013 May;25(5):1523-40. (PMID: 23667124)
Nucleic Acids Res. 2000 Jan 1;28(1):27-30. (PMID: 10592173)
Cell Tissue Res. 1989 Jul;257(1):93-103. (PMID: 2752416)
Biochim Biophys Acta. 2004 Nov 29;1695(1-3):55-72. (PMID: 15571809)
Nucleic Acids Res. 2007 Jul;35(Web Server issue):W585-7. (PMID: 17517783)
Hum Reprod. 2015 Nov;30(11):2597-613. (PMID: 26345691)
Development. 1997 Nov;124(22):4595-603. (PMID: 9409676)
فهرسة مساهمة: Keywords: LC-MS/MS; buffalo testis; lysine ubiquitination; post translational modification; proteomics; spermatogenesis
المشرفين على المادة: 0 (Proteins)
0 (Proteome)
K3Z4F929H6 (Lysine)
تواريخ الأحداث: Date Created: 20200530 Date Completed: 20210323 Latest Revision: 20210323
رمز التحديث: 20240829
مُعرف محوري في PubMed: PMC7298129
DOI: 10.1042/BSR20193537
PMID: 32469046
قاعدة البيانات: MEDLINE
الوصف
تدمد:1573-4935
DOI:10.1042/BSR20193537