دورية أكاديمية
Quercetin increases bioavailability of nitric oxide in the jejunum of euglycemic and diabetic rats and induces neuronal plasticity in the myenteric plexus.
| العنوان: | Quercetin increases bioavailability of nitric oxide in the jejunum of euglycemic and diabetic rats and induces neuronal plasticity in the myenteric plexus. |
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| المؤلفون: | Martins-Perles JVC; Department of Morphological Sciences, Universidade Estadual de Maringá, Avenida Colombo, n 5790 Bloco O-33, Maringá, PR CEP 87020-900, Brazil., Bossolani GDP; Department of Morphological Sciences, Universidade Estadual de Maringá, Avenida Colombo, n 5790 Bloco O-33, Maringá, PR CEP 87020-900, Brazil., Zignani I; Department of Morphological Sciences, Universidade Estadual de Maringá, Avenida Colombo, n 5790 Bloco O-33, Maringá, PR CEP 87020-900, Brazil., de Souza SRG; Department of Morphological Sciences, Universidade Estadual de Maringá, Avenida Colombo, n 5790 Bloco O-33, Maringá, PR CEP 87020-900, Brazil., Frez FCV; Department of Morphological Sciences, Universidade Estadual de Maringá, Avenida Colombo, n 5790 Bloco O-33, Maringá, PR CEP 87020-900, Brazil., de Souza Melo CG; Department of Morphological Sciences, Universidade Estadual de Maringá, Avenida Colombo, n 5790 Bloco O-33, Maringá, PR CEP 87020-900, Brazil., Barili E; Department of Statistic, Universidade Estadual de Maringá, Avenida Colombo, n 5790, Maringá, PR CEP 87020-900, Brazil., de Souza Neto FP; Department of Pathology Sciences, Universidade Estadual de Londrina (UEL), Rodovia Celso Garcia Cid|Pr 445 Km 380, Londrina, PR CEP 86.057-970, Brazil., Guarnier FA; Department of Pathology Sciences, Universidade Estadual de Londrina (UEL), Rodovia Celso Garcia Cid|Pr 445 Km 380, Londrina, PR CEP 86.057-970, Brazil., Armani ALC; Department of Pathology Sciences, Universidade Estadual de Londrina (UEL), Rodovia Celso Garcia Cid|Pr 445 Km 380, Londrina, PR CEP 86.057-970, Brazil., Cecchini R; Department of Pathology Sciences, Universidade Estadual de Londrina (UEL), Rodovia Celso Garcia Cid|Pr 445 Km 380, Londrina, PR CEP 86.057-970, Brazil., Zanoni JN; Department of Morphological Sciences, Universidade Estadual de Maringá, Avenida Colombo, n 5790 Bloco O-33, Maringá, PR CEP 87020-900, Brazil. Electronic address: jnzanoni@uem.br. |
| المصدر: | Autonomic neuroscience : basic & clinical [Auton Neurosci] 2020 Sep; Vol. 227, pp. 102675. Date of Electronic Publication: 2020 May 06. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 100909359 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7484 (Electronic) Linking ISSN: 15660702 NLM ISO Abbreviation: Auton Neurosci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Amsterdam ; New York] : Elsevier, c2000- |
| مواضيع طبية MeSH: | Antioxidants/*pharmacology , Diabetes Mellitus, Experimental/*drug therapy , Jejunum/*drug effects , Myenteric Plexus/*drug effects , Neuronal Plasticity/*drug effects , Neurons/*drug effects , Nitric Oxide/*metabolism , Nitric Oxide Synthase Type I/*drug effects , Quercetin/*pharmacology , Vasoactive Intestinal Peptide/*drug effects, Animals ; Antioxidants/administration & dosage ; Diabetes Mellitus, Experimental/metabolism ; Diabetes Mellitus, Experimental/pathology ; Male ; Myenteric Plexus/pathology ; Quercetin/administration & dosage ; Rats ; Rats, Wistar |
| مستخلص: | Considering the antioxidant, neuroprotective, inflammatory and nitric oxide modulatory actions of quercetin, the aim of this study was to test the effect of quercetin administration in drinking water (40 mg/day/rat) on neuronal nitric oxide synthase (nNOS), vasoactive intestinal peptide (VIP), overall population of myenteric neurons (HuC/D) and nitric oxide (NO) levels in the jejunal samples from diabetic rats. Male Wistar rats were distributed into four groups (8 rats per group): euglycemic (E), euglycemic administered with quercetin (E+Q), diabetic (D) and diabetic administered with quercetin (D+Q). Rats were induced to diabetes with streptozotocin (35mg/kg/iv) and, after 120 days, the proximal jejunum were collected and processed for immunohistochemical (VIP, nNOS and HuC/D) and chemiluminescence (quantification of tissue NO levels) techniques. Diabetes mellitus reduced the number of nNOS-IR (immunoreactive) (p <0.05) and HuC/D-IR (p <0.001) neurons, however, promoted an increased morphometric area of nNOS-IR neurons (p <0.001) and VIP-IR varicosities (p <0.05). In D+Q group, neuroplasticity effects were observed on HuC/D-IR neurons, accompanied by a reduction of cell body area of neurons nNOS- and VIP-IR varicosities (p <0.05). The NO levels were increased in the E+Q (p <0.05) and D+Q group (p <0.001) compared to the control group. In conclusion, the results showed that quercetin supplementation increased the bioavailability of NO in the jejunum in euglycemic and mitigate the effects of diabetes on nNOS-IR neurons and VIP-IR varicosities in the myenteric plexus of diabetic rats. (Copyright © 2020 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Diabetes mellitus; Myenteric varicosities; Neuroplasticity; VIP |
| المشرفين على المادة: | 0 (Antioxidants) 31C4KY9ESH (Nitric Oxide) 37221-79-7 (Vasoactive Intestinal Peptide) 9IKM0I5T1E (Quercetin) EC 1.14.13.39 (Nitric Oxide Synthase Type I) |
| تواريخ الأحداث: | Date Created: 20200601 Date Completed: 20210524 Latest Revision: 20210524 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.autneu.2020.102675 |
| PMID: | 32474374 |
| قاعدة البيانات: | MEDLINE |
Full Text via ClinicalKey 
Full Text Finder | تدمد: | 1872-7484 |
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| DOI: | 10.1016/j.autneu.2020.102675 |