دورية أكاديمية
The methylation status of TNF-α and SOCS3 promoters and the regulation of these gene expressions in patients with Behçet's disease.
| العنوان: | The methylation status of TNF-α and SOCS3 promoters and the regulation of these gene expressions in patients with Behçet's disease. |
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| المؤلفون: | Aziz SG; Department of Biochemistry, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran., Aziz SG; Department of Food Science and Technology, College of Agriculture, Tabriz Branch, Islamic Azad University, Tabriz, Iran., Khabbazi A; Connective Tissue Disease, Tabriz University of Medical Science, Tabriz, Iran., Alipour S; Department of Biochemistry, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran. |
| المصدر: | Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals [Biomarkers] 2020 Jul; Vol. 25 (5), pp. 384-390. Date of Electronic Publication: 2020 Jun 01. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Tayor & Francis Country of Publication: England NLM ID: 9606000 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1366-5804 (Electronic) Linking ISSN: 1354750X NLM ISO Abbreviation: Biomarkers Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : Abingdon, Oxford : Tayor & Francis Original Publication: London : Taylor & Francis, c1996- |
| مواضيع طبية MeSH: | Genetic Predisposition to Disease*, Behcet Syndrome/*genetics , Suppressor of Cytokine Signaling 3 Protein/*genetics , Tumor Necrosis Factor-alpha/*genetics, Adult ; Behcet Syndrome/pathology ; Biomarkers/metabolism ; Case-Control Studies ; DNA Methylation/genetics ; Female ; Gene Expression Regulation/genetics ; Humans ; Male ; Middle Aged ; Promoter Regions, Genetic/genetics |
| مستخلص: | Introduction: The aim of this study was to evaluate the methylation status of TNF-α and SOCS3 promoters in patients with BD and compare them with a healthy group. Method: This was a case-control study, in which 47 subjects with BD and 61 individuals as the control participated. Blood samples were collected from all the participants. Then, PBMCs were isolated using the Ficoll method and methylation of considered sites was investigated using the qMS-PCR technique after DNA extraction by the rapid genomic DNA extraction method and its analysis with Nano-drop. Results: The methylation and expression of TNF-α showed that the methylation level significantly declined in the patient in comparison with the healthy ( p < 0.05). Moreover, the results on the mean expression showed that it significantly increased in the patient group, as compared with the healthy group ( p < 0.05). In addition, the expression of the SOCS3 gene was not significantly different between the patients and healthy subjects while the level of SOCS3 methylation was significantly higher in the patient group than that in the healthy group ( p < 0.05). Discussion: The present study revealed that the gene expression of TNF-alpha increased in BD patients, suggesting that TNF-alpha likely has a role in the pathogenesis of BD. |
| فهرسة مساهمة: | Keywords: Behcet’s disease; DNA methylation; SOCS3; TNF-α; qMS-PCR |
| المشرفين على المادة: | 0 (Biomarkers) 0 (SOCS3 protein, human) 0 (Suppressor of Cytokine Signaling 3 Protein) 0 (Tumor Necrosis Factor-alpha) |
| تواريخ الأحداث: | Date Created: 20200602 Date Completed: 20210414 Latest Revision: 20210414 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1080/1354750X.2020.1754912 |
| PMID: | 32475174 |
| قاعدة البيانات: | MEDLINE |
PDF full text from Publisher | تدمد: | 1366-5804 |
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| DOI: | 10.1080/1354750X.2020.1754912 |