دورية أكاديمية
LPS restores protective immunity in macrophages against Mycobacterium tuberculosis via autophagy.
| العنوان: | LPS restores protective immunity in macrophages against Mycobacterium tuberculosis via autophagy. |
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| المؤلفون: | Fang F; Anhui Provincial Key Laboratory of Immunology in Chronic Diseases, Anhui Provincial Key Laboratory of Infection and Immunology, and Department of Immunogy, Bengbu Medical College, Bengbu, Anhui 233030, China., Ge Q; Anhui Provincial Key Laboratory of Immunology in Chronic Diseases, Anhui Provincial Key Laboratory of Infection and Immunology, and Department of Immunogy, Bengbu Medical College, Bengbu, Anhui 233030, China., Li R; Anhui Provincial Key Laboratory of Immunology in Chronic Diseases, Anhui Provincial Key Laboratory of Infection and Immunology, and Department of Immunogy, Bengbu Medical College, Bengbu, Anhui 233030, China., Lv J; Anhui Provincial Key Laboratory of Immunology in Chronic Diseases, Anhui Provincial Key Laboratory of Infection and Immunology, and Department of Immunogy, Bengbu Medical College, Bengbu, Anhui 233030, China., Zhang Y; Anhui Provincial Key Laboratory of Immunology in Chronic Diseases, Anhui Provincial Key Laboratory of Infection and Immunology, and Department of Immunogy, Bengbu Medical College, Bengbu, Anhui 233030, China; Department of Internal Medicine, University of Arizona, Phoenix, AZ, 85004, USA., Feng A; Department of Internal Medicine, University of Arizona, Phoenix, AZ, 85004, USA., Kelly GT; Department of Internal Medicine, University of Arizona, Phoenix, AZ, 85004, USA., Wang H; Anhui Provincial Key Laboratory of Immunology in Chronic Diseases, Anhui Provincial Key Laboratory of Infection and Immunology, and Department of Immunogy, Bengbu Medical College, Bengbu, Anhui 233030, China., Wang X; Anhui Clinical and Preclinical Key Laboratory of Respiratory Disease, Department of Respiration, First Affiliated Hospital, Bengbu Medical College, Bengbu, Anhui 233000, China., Song C; Anhui Provincial Key Laboratory of Immunology in Chronic Diseases, Anhui Provincial Key Laboratory of Infection and Immunology, and Department of Immunogy, Bengbu Medical College, Bengbu, Anhui 233030, China., Wang T; Department of Internal Medicine, University of Arizona, Phoenix, AZ, 85004, USA. Electronic address: twang@email.arizona.edu., Qian Z; Anhui Provincial Key Laboratory of Immunology in Chronic Diseases, Anhui Provincial Key Laboratory of Infection and Immunology, and Department of Immunogy, Bengbu Medical College, Bengbu, Anhui 233030, China. Electronic address: qzq7778@bbmc.edu.cn. |
| المصدر: | Molecular immunology [Mol Immunol] 2020 Aug; Vol. 124, pp. 18-24. Date of Electronic Publication: 2020 May 30. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Pergamon Press Country of Publication: England NLM ID: 7905289 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-9142 (Electronic) Linking ISSN: 01615890 NLM ISO Abbreviation: Mol Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Oxford, Elmsford, N. Y., Pergamon Press. |
| مواضيع طبية MeSH: | Autophagy/*immunology , Macrophages/*immunology , Mycobacterium tuberculosis/*immunology , Tuberculosis/*immunology, Autophagy/drug effects ; Humans ; Lipopolysaccharides/immunology ; Lipopolysaccharides/pharmacology |
| مستخلص: | Autophagy has been identified as an important immune regulatory mechanism. Recent studies have linked macrophage autophagy with innate immune responses against Mycobacterium tuberculosis (M. tuberculosis), which can survive within macrophages by blocking fusion of the phagosome with lysosomes. These findings suggest that autophagy is a regulatable cellular mechanism of M. tuberculosis defense in macrophages. Transcriptomic profiles in human blood in TB patients suggest that M. tuberculosis affects autophagy related pathways. In order to better understand the role of macrophage autophagy in enhancing protective immunity against M. tuberculosis, in this study, we investigate the effects of the autophagy activators rapamycin and LPS in macrophage autophagy and immunity against M. tuberculosis. We confirm that rapamycin and LPS induce autophagy in M. tuberculosis infected THP-1-derived macrophages or PMA primed THP-1 macrophages [THP-1(A)]. LPS restores M. tuberculosis-inhibited IL-12 synthesis and secretion in THP-1(A) cells via autophagy. Similarly, autophagy activators increase IL-12 synthesis and secretion in THP-1(A) cells. These studies demonstrate the importance of autophagy in M. tuberculosis elimination in macrophages and may lead to novel therapies for tuberculosis and other bacterial infections. Competing Interests: Declaration of Competing Interest The authors declare no competing interest. (Copyright © 2020 The Authors. Published by Elsevier Ltd.. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Autophagy; IFN-γ; IL-12; Mycobacterium tuberculosis |
| المشرفين على المادة: | 0 (Lipopolysaccharides) |
| تواريخ الأحداث: | Date Created: 20200603 Date Completed: 20200904 Latest Revision: 20200904 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.molimm.2020.05.001 |
| PMID: | 32485435 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1872-9142 |
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| DOI: | 10.1016/j.molimm.2020.05.001 |