دورية أكاديمية
Profiling of gene expression in methicillin-resistant Staphylococcus aureus in response to cyclo-(L-Val-L-Pro) and chloramphenicol isolated from Streptomyces sp., SUK 25 reveals gene downregulation in multiple biological targets.
العنوان: | Profiling of gene expression in methicillin-resistant Staphylococcus aureus in response to cyclo-(L-Val-L-Pro) and chloramphenicol isolated from Streptomyces sp., SUK 25 reveals gene downregulation in multiple biological targets. |
---|---|
المؤلفون: | Zin NM; Programme of Biomedical Science, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300, Kuala Lumpur, Malaysia., Al-Shaibani MM; Programme of Biomedical Science, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300, Kuala Lumpur, Malaysia. muhanna@imas.edu.my., Jalil J; Drug and Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300, Kuala Lumpur, Malaysia., Sukri A; Programme of Biomedical Science, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300, Kuala Lumpur, Malaysia., Al-Maleki AR; Medical Microbiology Department, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.; Department of Medical Microbiology, Faculty of Medicine and Health Sciences, Sana'a University, Sana'a, Yemen., Sidik NM; Faculty of Agro-Based Industry, Universiti Malaysia Kelantan, 17600, Jeli, Kelantan, Malaysia. nikmarzuki@umk.edu.my. |
المصدر: | Archives of microbiology [Arch Microbiol] 2020 Oct; Vol. 202 (8), pp. 2083-2092. Date of Electronic Publication: 2020 Jun 03. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 0410427 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-072X (Electronic) Linking ISSN: 03028933 NLM ISO Abbreviation: Arch Microbiol Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Berlin, New York, Springer-Verlag. |
مواضيع طبية MeSH: | Chloramphenicol/*pharmacology , Down-Regulation/*drug effects , Methicillin-Resistant Staphylococcus aureus/*drug effects , Methicillin-Resistant Staphylococcus aureus/*genetics, Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Gene Expression Profiling ; Microbial Sensitivity Tests ; Staphylococcal Infections/drug therapy ; Streptomyces/chemistry |
مستخلص: | Chloramphenicol (CAP) and cyclo-(L-Val-L-Pro) were previously isolated from Streptomyces sp., SUK 25 which exhibited a high potency against methicillin-resistant Staphylococcus aureus (MRSA). This study aimed to profile gene expression of MRSA treated with CAP and cyclo-(L-Val-L-Pro) compounds using DNA microarray. Treatment of MRSA with CAP resulted in upregulation of genes involved in protein synthesis, suggesting the coping mechanism of MRSA due to the inhibition of protein synthesis effect from CAP. Most upregulated genes in cyclo-(L-Val-L-Pro) were putative genes with unknown functions. Interestingly, genes encoding ribosomal proteins, cell membrane synthesis, DNA metabolism, citric acid cycle and virulence were downregulated in MRSA treated with cyclo-(L-Val-L-Pro) compound, suggesting the efficacy of this compound in targeting multiple biological pathways. Contrary to CAP, with only a single target, cyclo-(L-Val-L-Pro) isolated from this study had multiple antimicrobial targets that can delay antibiotic resistance and hence is a potential antimicrobial agent of MRSA. |
معلومات مُعتمدة: | MI-2018-004 Universiti Kebangsaan Malaysia; FRGS/1/2011/ST/UKM/02/1 Ministry of Higher Education |
فهرسة مساهمة: | Keywords: Chloramphenicol; Cyclo-(L-val-L-pro); DNA microarray; MRSA; Streptomyces SUK 25 |
المشرفين على المادة: | 0 (Anti-Bacterial Agents) 66974FR9Q1 (Chloramphenicol) |
تواريخ الأحداث: | Date Created: 20200605 Date Completed: 20201008 Latest Revision: 20201008 |
رمز التحديث: | 20221213 |
DOI: | 10.1007/s00203-020-01896-x |
PMID: | 32494868 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1432-072X |
---|---|
DOI: | 10.1007/s00203-020-01896-x |