دورية أكاديمية
Dynamic proteomic analysis of Aedes aegypti Aag-2 cells infected with Mayaro virus.
العنوان: | Dynamic proteomic analysis of Aedes aegypti Aag-2 cells infected with Mayaro virus. |
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المؤلفون: | Vasconcellos AF; Laboratory of Protein Chemistry and Biochemistry, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, DF, 70910-900, Brazil.; Laboratory of Virology, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, DF, 70910-900, Brazil., Mandacaru SC; Laboratory of Protein Chemistry and Biochemistry, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, DF, 70910-900, Brazil., de Oliveira AS; Laboratory of Virology, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, DF, 70910-900, Brazil., Fontes W; Laboratory of Protein Chemistry and Biochemistry, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, DF, 70910-900, Brazil., Melo RM; Laboratory of Protein Chemistry and Biochemistry, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, DF, 70910-900, Brazil., de Sousa MV; Laboratory of Protein Chemistry and Biochemistry, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, DF, 70910-900, Brazil., Resende RO; Laboratory of Virology, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, DF, 70910-900, Brazil. rresende@unb.br., Charneau S; Laboratory of Protein Chemistry and Biochemistry, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, DF, 70910-900, Brazil. charneau@unb.br. |
المصدر: | Parasites & vectors [Parasit Vectors] 2020 Jun 10; Vol. 13 (1), pp. 297. Date of Electronic Publication: 2020 Jun 10. |
نوع المنشور: | Comparative Study; Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: BioMed Central Country of Publication: England NLM ID: 101462774 Publication Model: Electronic Cited Medium: Internet ISSN: 1756-3305 (Electronic) Linking ISSN: 17563305 NLM ISO Abbreviation: Parasit Vectors Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: London : BioMed Central |
مواضيع طبية MeSH: | Aedes/*cytology , Aedes/*virology , Arboviruses/*physiology , Host Microbial Interactions/*genetics , Proteomics/*methods, Animals ; Arboviruses/genetics ; Cell Line ; Energy Metabolism ; Insect Proteins/analysis ; Insect Proteins/genetics ; Mosquito Vectors/virology ; Virus Replication |
مستخلص: | Background: Mayaro virus (MAYV) is responsible for a mosquito-borne tropical disease with clinical symptoms similar to dengue or chikungunya virus fevers. In addition to the recent territorial expansion of MAYV, this virus may be responsible for an increasing number of outbreaks. Currently, no vaccine is available. Aedes aegypti is promiscuous in its viral transmission and thus an interesting model to understand MAYV-vector interactions. While the life-cycle of MAYV is known, the mechanisms by which this arbovirus affects mosquito host cells are not clearly understood. Methods: After defining the best conditions for cell culture harvesting using the highest virus titer, Ae. aegypti Aag-2 cells were infected with a Brazilian MAYV isolate at a MOI of 1 in order to perform a comparative proteomic analysis of MAYV-infected Aag-2 cells by using a label-free semi-quantitative bottom-up proteomic analysis. Time-course analyses were performed at 12 and 48 h post-infection (hpi). After spectrum alignment between the triplicates of each time point and changes of the relative abundance level calculation, the identified proteins were annotated and using Gene Ontology database and protein pathways were annotated using the Kyoto Encyclopedia of Genes and Genomes. Results: After three reproducible biological replicates, the total proteome analysis allowed for the identification of 5330 peptides and the mapping of 459, 376 and 251 protein groups, at time 0, 12 hpi and 48 hpi, respectively. A total of 161 mosquito proteins were found to be differentially abundant during the time-course, mostly related to host cell processes, including redox metabolism, translation, energy metabolism, and host cell defense. MAYV infection also increased host protein expression implicated in viral replication. Conclusions: To our knowledge, this first proteomic time-course analysis of MAYV-infected mosquito cells sheds light on the molecular basis of the viral infection process and host cell response during the first 48 hpi. Our data highlight several mosquito proteins modulated by the virus, revealing that MAYV manipulates mosquito cell metabolism for its propagation. |
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معلومات مُعتمدة: | Demanda Induzida - Aedes Aegypti e as arboviroses Zika Fundação de Apoio à Pesquisa do Distrito Federal; Chikungunya e Dengue 0193.000417/2016 Fundação de Apoio à Pesquisa do Distrito Federal; Demanda Espontânea 193.001.723/2017 Fundação de Apoio à Pesquisa do Distrito Federal; fellowship Fundação de Apoio à Pesquisa do Distrito Federal; Universal 430610/2016-5 Conselho Nacional de Desenvolvimento Científico e Tecnológico; fellowship Conselho Nacional de Desenvolvimento Científico e Tecnológico; PROEX Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; CT-Infra grants 0439/11 Financiadora de Estudos e Projetos; 0694/13 Financiadora de Estudos e Projetos; grant 923/18 CAPES-COFECUB Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (BR) |
فهرسة مساهمة: | Keywords: Differentially expressed proteins; Infection; MAYV; Mosquito; Semi-quantitative proteomics; Virus-vector interaction |
المشرفين على المادة: | 0 (Insect Proteins) |
تواريخ الأحداث: | Date Created: 20200612 Date Completed: 20210210 Latest Revision: 20240328 |
رمز التحديث: | 20240329 |
مُعرف محوري في PubMed: | PMC7285477 |
DOI: | 10.1186/s13071-020-04167-2 |
PMID: | 32522239 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1756-3305 |
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DOI: | 10.1186/s13071-020-04167-2 |