دورية أكاديمية
أعرض في EDS

Dynamic proteomic analysis of Aedes aegypti Aag-2 cells infected with Mayaro virus.

التفاصيل البيبلوغرافية
العنوان: Dynamic proteomic analysis of Aedes aegypti Aag-2 cells infected with Mayaro virus.
المؤلفون: Vasconcellos AF; Laboratory of Protein Chemistry and Biochemistry, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, DF, 70910-900, Brazil.; Laboratory of Virology, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, DF, 70910-900, Brazil., Mandacaru SC; Laboratory of Protein Chemistry and Biochemistry, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, DF, 70910-900, Brazil., de Oliveira AS; Laboratory of Virology, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, DF, 70910-900, Brazil., Fontes W; Laboratory of Protein Chemistry and Biochemistry, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, DF, 70910-900, Brazil., Melo RM; Laboratory of Protein Chemistry and Biochemistry, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, DF, 70910-900, Brazil., de Sousa MV; Laboratory of Protein Chemistry and Biochemistry, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, DF, 70910-900, Brazil., Resende RO; Laboratory of Virology, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, DF, 70910-900, Brazil. rresende@unb.br., Charneau S; Laboratory of Protein Chemistry and Biochemistry, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, DF, 70910-900, Brazil. charneau@unb.br.
المصدر: Parasites & vectors [Parasit Vectors] 2020 Jun 10; Vol. 13 (1), pp. 297. Date of Electronic Publication: 2020 Jun 10.
نوع المنشور: Comparative Study; Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101462774 Publication Model: Electronic Cited Medium: Internet ISSN: 1756-3305 (Electronic) Linking ISSN: 17563305 NLM ISO Abbreviation: Parasit Vectors Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central
مواضيع طبية MeSH: Aedes/*cytology , Aedes/*virology , Arboviruses/*physiology , Host Microbial Interactions/*genetics , Proteomics/*methods, Animals ; Arboviruses/genetics ; Cell Line ; Energy Metabolism ; Insect Proteins/analysis ; Insect Proteins/genetics ; Mosquito Vectors/virology ; Virus Replication
مستخلص: Background: Mayaro virus (MAYV) is responsible for a mosquito-borne tropical disease with clinical symptoms similar to dengue or chikungunya virus fevers. In addition to the recent territorial expansion of MAYV, this virus may be responsible for an increasing number of outbreaks. Currently, no vaccine is available. Aedes aegypti is promiscuous in its viral transmission and thus an interesting model to understand MAYV-vector interactions. While the life-cycle of MAYV is known, the mechanisms by which this arbovirus affects mosquito host cells are not clearly understood.
Methods: After defining the best conditions for cell culture harvesting using the highest virus titer, Ae. aegypti Aag-2 cells were infected with a Brazilian MAYV isolate at a MOI of 1 in order to perform a comparative proteomic analysis of MAYV-infected Aag-2 cells by using a label-free semi-quantitative bottom-up proteomic analysis. Time-course analyses were performed at 12 and 48 h post-infection (hpi). After spectrum alignment between the triplicates of each time point and changes of the relative abundance level calculation, the identified proteins were annotated and using Gene Ontology database and protein pathways were annotated using the Kyoto Encyclopedia of Genes and Genomes.
Results: After three reproducible biological replicates, the total proteome analysis allowed for the identification of 5330 peptides and the mapping of 459, 376 and 251 protein groups, at time 0, 12 hpi and 48 hpi, respectively. A total of 161 mosquito proteins were found to be differentially abundant during the time-course, mostly related to host cell processes, including redox metabolism, translation, energy metabolism, and host cell defense. MAYV infection also increased host protein expression implicated in viral replication.
Conclusions: To our knowledge, this first proteomic time-course analysis of MAYV-infected mosquito cells sheds light on the molecular basis of the viral infection process and host cell response during the first 48 hpi. Our data highlight several mosquito proteins modulated by the virus, revealing that MAYV manipulates mosquito cell metabolism for its propagation.
References: mBio. 2017 Feb 14;8(1):. (PMID: 28196962)
PLoS Negl Trop Dis. 2015 Mar 04;9(3):e0003544. (PMID: 25738640)
Mem Inst Oswaldo Cruz. 2013;108 Suppl 1:11-7. (PMID: 24473798)
Mol Cell. 2010 Jul 30;39(2):292-9. (PMID: 20605501)
Bioinformatics. 2018 Sep 1;34(17):2965-2972. (PMID: 29635359)
Rev Soc Bras Med Trop. 2014 Nov-Dec;47(6):677-83. (PMID: 25626645)
PLoS Negl Trop Dis. 2010 Oct 26;4(10):e856. (PMID: 21049065)
An Acad Bras Cienc. 2016 Sep;88(3):1485-99. (PMID: 27627069)
Proteomics. 2015 Mar;15(5-6):930-49. (PMID: 25158685)
J Virol. 2015 Feb;89(4):2358-66. (PMID: 25505078)
Vector Borne Zoonotic Dis. 2012 Jan;12(1):42-6. (PMID: 21923266)
BMC Syst Biol. 2010 May 13;4:61. (PMID: 20465796)
BMC Genomics. 2007 Jul 11;8:231. (PMID: 17625007)
J Biosci. 2008 Nov;33(4):443-9. (PMID: 19208970)
J Med Virol. 2012 Nov;84(11):1757-70. (PMID: 22997079)
Sci Rep. 2017 Aug 18;7(1):8718. (PMID: 28821712)
Nat Biotechnol. 2014 Mar;32(3):223-6. (PMID: 24727771)
Sci Rep. 2015 Aug 03;5:12758. (PMID: 26234525)
Proc Natl Acad Sci U S A. 2002 Oct 15;99(21):13867-72. (PMID: 12364587)
Nat Immunol. 2013 Apr;14(4):396-403. (PMID: 23435119)
Viruses. 2015 Jul 08;7(7):3741-67. (PMID: 26184281)
J Environ Sci (China). 2008;20(3):309-19. (PMID: 18595398)
Nature. 2014 Nov 13;515(7526):222-7. (PMID: 25391959)
Genes Dev. 1997 Dec 1;11(23):3122-7. (PMID: 9389645)
Res Virol. 1991 Jan-Feb;142(1):25-31. (PMID: 1711236)
Intractable Rare Dis Res. 2016 May;5(2):90-6. (PMID: 27195191)
Emerg Microbes Infect. 2018 Sep 26;7(1):163. (PMID: 30254258)
Parasit Vectors. 2011 Jul 18;4:138. (PMID: 21767351)
Braz J Microbiol. 2016 Dec;47 Suppl 1:38-50. (PMID: 27818091)
Annu Rev Immunol. 1990;8:401-20. (PMID: 2188668)
Nature. 1992 Jan 2;355(6355):33-45. (PMID: 1731198)
Virology. 2015 May;479-480:609-18. (PMID: 25812764)
Nucleic Acids Res. 2018 Jul 2;46(W1):W486-W494. (PMID: 29762782)
Curr Protoc Bioinformatics. 2017 Sep 13;59:13.31.1-13.31.12. (PMID: 28902400)
Virology. 2009 Oct 25;393(2):183-97. (PMID: 19732931)
Viruses. 2013 May 22;5(5):1272-91. (PMID: 23698399)
Trop Med Health. 2011 Dec;39(4 Suppl):37-43. (PMID: 22500135)
Arch Gesamte Virusforsch. 1973;40(3):382-9. (PMID: 4735533)
معلومات مُعتمدة: Demanda Induzida - Aedes Aegypti e as arboviroses Zika Fundação de Apoio à Pesquisa do Distrito Federal; Chikungunya e Dengue 0193.000417/2016 Fundação de Apoio à Pesquisa do Distrito Federal; Demanda Espontânea 193.001.723/2017 Fundação de Apoio à Pesquisa do Distrito Federal; fellowship Fundação de Apoio à Pesquisa do Distrito Federal; Universal 430610/2016-5 Conselho Nacional de Desenvolvimento Científico e Tecnológico; fellowship Conselho Nacional de Desenvolvimento Científico e Tecnológico; PROEX Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; CT-Infra grants 0439/11 Financiadora de Estudos e Projetos; 0694/13 Financiadora de Estudos e Projetos; grant 923/18 CAPES-COFECUB Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (BR)
فهرسة مساهمة: Keywords: Differentially expressed proteins; Infection; MAYV; Mosquito; Semi-quantitative proteomics; Virus-vector interaction
المشرفين على المادة: 0 (Insect Proteins)
تواريخ الأحداث: Date Created: 20200612 Date Completed: 20210210 Latest Revision: 20240328
رمز التحديث: 20240329
مُعرف محوري في PubMed: PMC7285477
DOI: 10.1186/s13071-020-04167-2
PMID: 32522239
قاعدة البيانات: MEDLINE
كن أول من يترك تعليقا!
يجب تسجيل دخولك أولا