دورية أكاديمية
أعرض في EDS

Xela DS2 and Xela VS2: Two novel skin epithelial-like cell lines from adult African clawed frog (Xenopus laevis) and their response to an extracellular viral dsRNA analogue.

التفاصيل البيبلوغرافية
العنوان: Xela DS2 and Xela VS2: Two novel skin epithelial-like cell lines from adult African clawed frog (Xenopus laevis) and their response to an extracellular viral dsRNA analogue.
المؤلفون: Bui-Marinos MP; Department of Biology, University of Waterloo, Waterloo, ON, N2L 3G1, Canada., Varga JFA; Department of Biology, University of Waterloo, Waterloo, ON, N2L 3G1, Canada., Vo NTK; Department of Biology, University of Waterloo, Waterloo, ON, N2L 3G1, Canada., Bols NC; Department of Biology, University of Waterloo, Waterloo, ON, N2L 3G1, Canada., Katzenback BA; Department of Biology, University of Waterloo, Waterloo, ON, N2L 3G1, Canada. Electronic address: barb.katzenback@uwaterloo.ca.
المصدر: Developmental and comparative immunology [Dev Comp Immunol] 2020 Nov; Vol. 112, pp. 103759. Date of Electronic Publication: 2020 Jun 08.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Science Country of Publication: United States NLM ID: 7708205 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0089 (Electronic) Linking ISSN: 0145305X NLM ISO Abbreviation: Dev Comp Immunol Subsets: MEDLINE
أسماء مطبوعة: Publication: Tarrytown Ny : Elsevier Science
Original Publication: Elmsford, N. Y., Pergamon Press., 1977-2004.
مواضيع طبية MeSH: Epithelial Cells/*cytology , Epithelial Cells/*immunology , Inflammation/*immunology , RNA, Viral/*immunology , Skin/*cytology , Virus Diseases/*immunology , Xenopus laevis/*physiology, Animals ; Cell Culture Techniques ; Cell Line ; Disease Models, Animal ; Humans ; Mice ; Pathogen-Associated Molecular Pattern Molecules/immunology ; Poly I-C/immunology ; RNA, Double-Stranded ; Xenopus laevis/virology
مستخلص: The skin epithelial layer acts as an important immunological barrier against pathogens and is capable of recognizing and responding to pathogen-associated molecular patterns (PAMPs) in human and mouse models. Although presumed, it is unknown whether amphibian skin epithelial cells exhibit the ability to respond to PAMPs such as viral double-stranded RNA (dsRNA). To address this, two cell lines from the dorsal skin (Xela DS2) and ventral skin (Xela VS2) of the African clawed frog (Xenopus laevis) were established. Xela DS2 and Xela VS2 cells have an epithelial-like morphology, express genes associated with epithelial cells, and lack senescence-associated beta-galactosidase activity. Cells grow optimally in 70% Leibovitz's L-15 medium supplemented with 15% fetal bovine serum at 26 °C. Upon treatment with poly(I:C), a synthetic analogue of viral dsRNA and known type I interferon inducer, Xela DS2 and Xela VS2 exhibit marked upregulation of key antiviral and pro-inflammatory transcripts suggesting frog epithelial cells participate in the recognition of extracellular viral dsRNA and production of local inflammatory signals; similar to human and mouse models. Currently, these are the only known Xenopus laevis skin epithelial-like cell lines and will be important for future research in amphibian epithelial cell biology, initial host-pathogen interactions, and rapid screening of the effects of environmental stressors, including contaminants, on frog skin epithelial cells.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: Amphibians; Antiviral response; Cell lines; Poly(I:C); Proinflammatory cytokines; Type I interferons
المشرفين على المادة: 0 (Pathogen-Associated Molecular Pattern Molecules)
0 (RNA, Double-Stranded)
0 (RNA, Viral)
O84C90HH2L (Poly I-C)
تواريخ الأحداث: Date Created: 20200612 Date Completed: 20210917 Latest Revision: 20210917
رمز التحديث: 20240628
DOI: 10.1016/j.dci.2020.103759
PMID: 32526291
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-0089
DOI:10.1016/j.dci.2020.103759