دورية أكاديمية
Croton urucurana Baillon stem bark ointment accelerates the closure of cutaneous wounds in knockout IL-10 mice.
| العنوان: | Croton urucurana Baillon stem bark ointment accelerates the closure of cutaneous wounds in knockout IL-10 mice. |
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| المؤلفون: | Casao TDRL; Department of Biochemistry and Molecular Biology, Viçosa Federal University, 35570-900, Viçosa, Minas Gerais, Brazil. Electronic address: thalia89.tlc@gmail.com., Pinheiro CG; Department of Biochemistry and Molecular Biology, Viçosa Federal University, 35570-900, Viçosa, Minas Gerais, Brazil. Electronic address: galpinheiro@gmail.com., Sarandy MM; Department of Animal Biology, Viçosa Federal University, 35570-900, Viçosa, Minas Gerais, Brazil. Electronic address: mariaureasarandy@gmail.com., Zanatta AC; Institute of Chemistry, São Paulo State University, Araraquara, 14800-900, São Paulo, Brazil. Electronic address: anaczanatta@gmail.com., Vilegas W; Institute of Biosciences, São Paulo State University, 05508-900, São Vicente, São Paulo, Brazil. Electronic address: vilegasw@gmail.com., Novaes RD; Department of Structural Biology, Federal University of Alfenas, 37130-001, Alfenas, Minas Gerais, Brazil. Electronic address: romuonovaes@yahoo.com.br., Gonçalves RV; Department of Animal Biology, Viçosa Federal University, 35570-900, Viçosa, Minas Gerais, Brazil. Electronic address: reggysvilela@yahoo.com.br., Viana Leite JP; Department of Biochemistry and Molecular Biology, Viçosa Federal University, 35570-900, Viçosa, Minas Gerais, Brazil. Electronic address: jpvleite@ufv.br. |
| المصدر: | Journal of ethnopharmacology [J Ethnopharmacol] 2020 Oct 28; Vol. 261, pp. 113042. Date of Electronic Publication: 2020 Jun 10. |
| نوع المنشور: | Comparative Study; Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Sequoia Country of Publication: Ireland NLM ID: 7903310 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7573 (Electronic) Linking ISSN: 03788741 NLM ISO Abbreviation: J Ethnopharmacol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Limerick : Elsevier Sequoia Original Publication: Lausanne, Elsevier Sequoia. |
| مواضيع طبية MeSH: | Croton*/chemistry , Plant Bark*/chemistry, Anti-Inflammatory Agents/*pharmacology , Antioxidants/*pharmacology , Interleukin-10/*metabolism , Plant Extracts/*pharmacology , Skin/*drug effects , Wound Healing/*drug effects , Wounds and Injuries/*drug therapy, Administration, Cutaneous ; Animals ; Anti-Inflammatory Agents/isolation & purification ; Antioxidants/isolation & purification ; Disease Models, Animal ; Inflammation Mediators/metabolism ; Interleukin-10/deficiency ; Interleukin-10/genetics ; Mice, Inbred C57BL ; Mice, Knockout ; Ointments ; Oxidative Stress/drug effects ; Plant Extracts/isolation & purification ; Skin/injuries ; Skin/metabolism ; Skin/pathology ; Time Factors ; Wounds and Injuries/genetics ; Wounds and Injuries/metabolism ; Wounds and Injuries/pathology |
| مستخلص: | Ethnopharmacological Relevance: Croton urucurana Baill. (Euphorbiaceae) is a plant used in Brazilian popular medicine for the treatment of wound healing, inflammatory diseases, gastritis, infections, and hemorrhoids. Aim: The present study aimed to evaluate the in vivo wound healing activity of an ointment based on ethanolic extract of C. urucurana stem bark, at concentrations of 5% and 10%, and to relate it with compounds that could be associated with this activity. Materials and Methods: Analyses by FIA-ESI-IT-MS n were carried out to investigate the chemical composition of C. urucurana. Knockout IL-10 (n = 60) mice and wild type C57 (n = 12) mice were separated into 6 groups to evaluate the wound healing activity. Knockout IL-10 mice: SAL (0.9% saline); BAS (ointment base); SS (1% silver sulfadiazine); CR1 (ointment with extract of C. urucurana 5%); CR2 (ointment with extract of C. urucurana 10%); and wild mice C57: SALC57 (Saline 0.9%). A circular wound with 10 mm in diameter was generated on the dorsal of the animals. Tissue specimen of the wounds were removed on days 7 and 14 of the treatment for histopathological, oxidative status and analyses of pro-and anti-inflammatory cytokines in scar tissue. Results: In the phytochemical profile, twelve proanthocyanidins were identified (in the form of monomers, dimers, trimers, and tetramers), based on (epi)catechin and (epi)gallocatechin. Furthermore, two quercetin derivatives and two alkaloids were detected. The groups treated with CR1 and CR2 ointments presented higher rate of wound closure, increased total number of cells, mast cells, blood vessels and higher deposition of type III and I collagen. In addition, they showed increased amount of pro-inflammatory cytokines (IL- 2 and IFN-γ), and anti-inflmatory cytokines (IL-4), on the 7th day of treatment. Conclusion: The results presented support the popular use of preparations based on the bark of C. urucurana as a healing compound. (Copyright © 2020 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Croton urucurana; Medicinal plant; Oxidative stress; Proanthocyanidins; Wound healing |
| المشرفين على المادة: | 0 (Anti-Inflammatory Agents) 0 (Antioxidants) 0 (IL10 protein, mouse) 0 (Inflammation Mediators) 0 (Ointments) 0 (Plant Extracts) 130068-27-8 (Interleukin-10) |
| تواريخ الأحداث: | Date Created: 20200613 Date Completed: 20210226 Latest Revision: 20210226 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.jep.2020.113042 |
| PMID: | 32531412 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1872-7573 |
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| DOI: | 10.1016/j.jep.2020.113042 |