دورية أكاديمية
A TLR9-adjuvanted vaccine formulated into dissolvable microneedle patches or cationic liposomes protects against leishmaniasis after skin or subcutaneous immunization.
| العنوان: | A TLR9-adjuvanted vaccine formulated into dissolvable microneedle patches or cationic liposomes protects against leishmaniasis after skin or subcutaneous immunization. |
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| المؤلفون: | Lanza JS; Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil; Antiparasite Chemotherapy, UMR 8076 CNRS BioCIS, Faculté de Pharmacie, Université Paris-Sud, Université Paris-Saclay, Chatenay-Malabry, France., Vucen S; School of Pharmacy, University College Cork, Cork, Ireland., Flynn O; School of Pharmacy, University College Cork, Cork, Ireland., Donadei A; School of Pharmacy, University College Cork, Cork, Ireland., Cojean S; Antiparasite Chemotherapy, UMR 8076 CNRS BioCIS, Faculté de Pharmacie, Université Paris-Sud, Université Paris-Saclay, Chatenay-Malabry, France., Loiseau PM; Antiparasite Chemotherapy, UMR 8076 CNRS BioCIS, Faculté de Pharmacie, Université Paris-Sud, Université Paris-Saclay, Chatenay-Malabry, France., Fernandes APSM; Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Frézard F; Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Moore AC; School of Pharmacy, University College Cork, Cork, Ireland; School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland. Electronic address: anne.moore@ucc.ie. |
| المصدر: | International journal of pharmaceutics [Int J Pharm] 2020 Aug 30; Vol. 586, pp. 119390. Date of Electronic Publication: 2020 Jun 12. |
| نوع المنشور: | Comparative Study; Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 7804127 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3476 (Electronic) Linking ISSN: 03785173 NLM ISO Abbreviation: Int J Pharm Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Elsevier/North-Holland Biomedical Press. |
| مواضيع طبية MeSH: | Adjuvants, Immunologic/*administration & dosage , Leishmaniasis/*prevention & control , Leishmaniasis Vaccines/*administration & dosage , Toll-Like Receptor 9/*immunology, Animals ; Antigens, Protozoan/administration & dosage ; Antigens, Protozoan/immunology ; Cations ; Female ; Immunization ; Injections, Subcutaneous ; Leishmaniasis Vaccines/pharmacokinetics ; Liposomes ; Mice ; Mice, Inbred BALB C ; Skin Absorption ; Transdermal Patch |
| مستخلص: | Re-emergence and geographic expansion of leishmaniasis is accelerating efforts to develop a safe and effective Leshmania vaccine. Vaccines using Leishmania recombinant antigens, such as LiHyp1, which is mostly present in the amastigote parasite form, are being developed as a next generation to crude killed parasite-based vaccines. The main objective of this work was to develop a LiHyp1-based vaccine and determine if it can induce protective immunity in BALB/c mice when administered using a dissolvable microneedle (DMN) patch by the skin route. The LiHyp1 antigen was incorporated into cationic liposomes (CL), with or without the TLR9 agonist, CpG. The LiHyp1-liposomal vaccines were characterized with respect to size, protein encapsulation rates and retention of their physical characteristics after incorporation into the DMN patch. DMN mechanical strength and skin penetration ability were tested. A vaccine composed of LiHyp1, CpG and liposomes and subcutaneously injected or a vaccine containing antigen and CpG in DMN patches, without liposomes, induced high antibody responses and significant levels of protection against L. donovani parasite infection. This study progresses the development of an efficacious leishmania vaccine by detailing promising vaccine formulations and skin delivery technologies and it addresses protective efficacy of a liposome-based dissolvable microneedle patch vaccine system. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2020 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: CpG; Dissolvable microneedle patch; Leishmania; Liposome; Skin immunisation; Vaccine |
| المشرفين على المادة: | 0 (Adjuvants, Immunologic) 0 (Antigens, Protozoan) 0 (Cations) 0 (Leishmaniasis Vaccines) 0 (Liposomes) 0 (Toll-Like Receptor 9) |
| تواريخ الأحداث: | Date Created: 20200617 Date Completed: 20210303 Latest Revision: 20210303 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.ijpharm.2020.119390 |
| PMID: | 32540349 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-3476 |
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| DOI: | 10.1016/j.ijpharm.2020.119390 |