دورية أكاديمية
أعرض في EDS

An age-independent gene signature for monitoring acute rejection in kidney transplantation.

التفاصيل البيبلوغرافية
العنوان: An age-independent gene signature for monitoring acute rejection in kidney transplantation.
المؤلفون: Shaw BI; Department of Surgery, Duke University Medical Center, Durham, United States., Cheng DK; Department of Pediatrics, Duke University Medical Center, Durham, United States., Acharya CR; Department of Surgery, Duke University Medical Center, Durham, United States., Ettenger RB; Department of Pediatrics, UCLA Mattel Children's Hospital, Los Angeles, United States., Lyerly HK; Department of Surgery, Duke University Medical Center, Durham, United States., Cheng Q; Department of Surgery, Duke University Medical Center, Durham, United States., Kirk AD; Department of Surgery, Duke University Medical Center, Durham, United States.; Department of Pediatrics, Duke University Medical Center, Durham, United States., Chambers ET; Department of Surgery, Duke University Medical Center, Durham, United States.; Department of Pediatrics, Duke University Medical Center, Durham, United States.
المصدر: Theranostics [Theranostics] 2020 May 25; Vol. 10 (15), pp. 6977-6986. Date of Electronic Publication: 2020 May 25 (Print Publication: 2020).
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Ivyspring International Publisher Country of Publication: Australia NLM ID: 101552395 Publication Model: eCollection Cited Medium: Internet ISSN: 1838-7640 (Electronic) Linking ISSN: 18387640 NLM ISO Abbreviation: Theranostics Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Wyoming, N.S.W. : Ivyspring International Publisher, 2011-
مواضيع طبية MeSH: Transcriptome*, Biomarkers/*blood , Computational Biology/*methods , Graft Rejection/*diagnosis , Kidney Transplantation/*adverse effects , Transplantation, Homologous/*methods, Adolescent ; Adult ; Age Factors ; Aged ; Biopsy ; Child ; Child, Preschool ; Female ; Graft Rejection/blood ; Graft Rejection/etiology ; Graft Rejection/pathology ; Humans ; Infant ; Male ; Middle Aged ; ROC Curve ; Young Adult
مستخلص: Acute rejection (AR) remains a significant problem that negatively impacts long-term renal allograft survival. Numerous therapies are used to prevent AR that differ by center and recipient age. This variability confounds diagnostic methods. Methods : To develop an age-independent gene signature for AR effective across a broad array of immunosuppressive regimens, we compiled kidney transplant biopsy (n=1091) and peripheral blood (n=392) gene expression profiles from 12 independent public datasets. After removing genes differentially expressed in pediatric and adult patients, we compared gene expression profiles from biopsy and peripheral blood samples of patients with AR to those who were stable (STA), using Mann-Whitney U Tests with validation in independent testing datasets. We confirmed this signature in pediatric and adult patients (42 AR and 47 STA) from our institutional biorepository. Results : We identified a novel age-independent gene network that identified AR from both kidney and blood samples. We developed a 90-probe set signature targeting 76 genes that differentiated AR from STA and found an 8 gene subset (DIP2C, ENOSF1, FBXO21, KCTD6, PDXDC1, REXO2, HLA-E, and RAB31) that was associated with AR. Conclusion : We used publicly available datasets to create a gene signature of AR that identified AR irrespective of immunosuppression regimen or recipient age. This study highlights a novel model to screen and validate biomarkers across multiple treatment regimens.
Competing Interests: Competing Interests: The authors have declared that no competing interest exists.
(© The author(s).)
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معلومات مُعتمدة: U01 AI077821 United States AI NIAID NIH HHS
فهرسة مساهمة: Keywords: acute rejection; big data; gene expression; kidney transplant; pediatrics
المشرفين على المادة: 0 (Biomarkers)
تواريخ الأحداث: Date Created: 20200620 Date Completed: 20210518 Latest Revision: 20210518
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC7295062
DOI: 10.7150/thno.42110
PMID: 32550916
قاعدة البيانات: MEDLINE
الوصف
تدمد:1838-7640
DOI:10.7150/thno.42110