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Multimeric TAT peptides are effective in vitro inhibitors of Staphylococcus saprophyticus.

التفاصيل البيبلوغرافية
العنوان: Multimeric TAT peptides are effective in vitro inhibitors of Staphylococcus saprophyticus.
المؤلفون: Chiongson JBV; Department of Chemistry, College of Science, De La Salle University, Manila, Philippines.; Department of Chemistry and Physics, College of Liberal Arts, Sciences and Education, University of San Agustin, Iloilo City, Philippines., Sabido EM; Center for Natural Drug Discovery and Development (CND3), University of San Agustin, Iloilo City, Philippines., Lin KG; Genomics Research Center, Academia Sinica, Taipei, Taiwan., Alea GV; Department of Chemistry, College of Science, De La Salle University, Manila, Philippines., Dalisay DS; Center for Chemical Biology and Biotechnology (C2B2), University of San Agustin, Iloilo City, Philippines., Wu SH; Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan., Saludes JP; Department of Chemistry and Physics, College of Liberal Arts, Sciences and Education, University of San Agustin, Iloilo City, Philippines.; Center for Natural Drug Discovery and Development (CND3), University of San Agustin, Iloilo City, Philippines.
المصدر: Chemical biology & drug design [Chem Biol Drug Des] 2020 Dec; Vol. 96 (6), pp. 1348-1354. Date of Electronic Publication: 2020 Jun 20.
نوع المنشور: Letter; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Wiley-Blackwell Country of Publication: England NLM ID: 101262549 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1747-0285 (Electronic) Linking ISSN: 17470277 NLM ISO Abbreviation: Chem Biol Drug Des Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Oxford : Wiley-Blackwell, 2006-
مواضيع طبية MeSH: Anti-Bacterial Agents/*pharmacology , Staphylococcus saprophyticus/*drug effects , tat Gene Products, Human Immunodeficiency Virus/*pharmacology, Amino Acid Sequence ; Anti-Bacterial Agents/chemistry ; Chromatography, High Pressure Liquid ; Humans ; Microbial Sensitivity Tests ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Staphylococcus saprophyticus/growth & development ; Structure-Activity Relationship ; tat Gene Products, Human Immunodeficiency Virus/chemistry
مستخلص: TAT (48-60) is a tridecapeptide from the envelope protein of HIV that was previously shown to possess cell-penetrating properties and antibacterial activity, making it a potential drug delivery agent for anticancer drugs and as antibacterial compound. Previous reports indicated that dimerization enhances the desired bioactivity of TAT; hence, we sought to synthesize multimeric TAT peptides. Herein, we describe the effects of multimerization on the antibacterial activity and secondary structure of the peptide. Terminal modifications such as N-acetylation and C-amidation were employed in the design. TATp monomer, dimer, and tetramer were synthesized using solid-phase peptide synthesis, purified by reversed-phase HPLC, and then characterized by mass spectrometry. Multimerization of the peptide did not change the secondary structure conformation. The CD analysis revealed a polyproline-II conformation for all peptide designs. Thus, this study provides a method of increasing the biological activity of the peptide by multimerization while retaining the secondary conformation of its monomeric unit. Furthermore, the bacteria Staphylococcus saprophyticus was found to be susceptible to the dimer and tetramer, with MIC 50 of 12.50 μm and <1.56 μm, respectively. This suggests a structure-activity relationship whereby the antibacterial activity increases with increase in valency.
(© 2020 John Wiley & Sons Ltd.)
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فهرسة مساهمة: Keywords: TAT; antibacterial peptide; cell-penetrating peptide; multimer; solid-phase peptide synthesis
المشرفين على المادة: 0 (Anti-Bacterial Agents)
0 (tat Gene Products, Human Immunodeficiency Virus)
تواريخ الأحداث: Date Created: 20200621 Date Completed: 20210817 Latest Revision: 20210817
رمز التحديث: 20231215
DOI: 10.1111/cbdd.13706
PMID: 32562357
قاعدة البيانات: MEDLINE
الوصف
تدمد:1747-0285
DOI:10.1111/cbdd.13706