دورية أكاديمية
Cloning of a SEPALLATA4-like gene (IiSEP4) in Isatis indigotica Fortune and characterization of its function in Arabidopsis thaliana.
| العنوان: | Cloning of a SEPALLATA4-like gene (IiSEP4) in Isatis indigotica Fortune and characterization of its function in Arabidopsis thaliana. |
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| المؤلفون: | Pu ZQ; Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), Shaanxi Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest University, Xi'an, 710069, China., Ma YY; Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), Shaanxi Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest University, Xi'an, 710069, China., Lu MX; Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), Shaanxi Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest University, Xi'an, 710069, China., Ma YQ; Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), Shaanxi Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest University, Xi'an, 710069, China., Xu ZQ; Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), Shaanxi Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest University, Xi'an, 710069, China. Electronic address: ziqinxu@nwu.edu.cn. |
| المصدر: | Plant physiology and biochemistry : PPB [Plant Physiol Biochem] 2020 Sep; Vol. 154, pp. 229-237. Date of Electronic Publication: 2020 Jun 10. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: France NLM ID: 9882449 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2690 (Electronic) Linking ISSN: 09819428 NLM ISO Abbreviation: Plant Physiol Biochem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Science Original Publication: Paris : Gauthier-Villars, c1987- |
| مواضيع طبية MeSH: | Gene Expression Regulation, Plant* , Isatis*/genetics, MADS Domain Proteins/*genetics , Plant Proteins/*genetics, Arabidopsis/genetics ; Cloning, Molecular ; Flowers/physiology ; MADS Domain Proteins/physiology ; Phylogeny ; Plant Proteins/physiology ; Plants, Genetically Modified |
| مستخلص: | E-class MADS-box genes, SEPALLATA (SEP), participate in various aspects of plant development together with B-, C- and D-class MADS-box genes. IiSEP4, a homologous gene of SEP4, was cloned from Isatis indigotica. IiSEP4 was highly expressed in sepals, and its mRNA was mildly detected in leaves, inflorescences, flowers, stamens and young silicles. Constitutive expression of IiSEP4 in Arabidopsis thaliana caused early flowering, accompanied by the reduction of flowers and floral organs. Moreover, the sepals in some flowers were transformed into carpelloid structures with stigmatic papillae, and obviously accompanied by ovule formation. Yeast two-hybrid assays demonstrated that IiSEP4 interacts with other woad MADS proteins to determine the identity of floral organs. These findings reveal the important roles of IiSEP4 in floral development of I. indigotica. The results of this study can lay a foundation for further study on biological functions of MADS transcriptional factors in I. indigotica. (Copyright © 2020 Elsevier Masson SAS. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Arabidopsis thaliana; Expression analysis; Floral development; IiSEP4; Isatis indigotica Fortune |
| المشرفين على المادة: | 0 (MADS Domain Proteins) 0 (Plant Proteins) |
| تواريخ الأحداث: | Date Created: 20200622 Date Completed: 20201210 Latest Revision: 20201214 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.plaphy.2020.05.031 |
| PMID: | 32563851 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-2690 |
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| DOI: | 10.1016/j.plaphy.2020.05.031 |