دورية أكاديمية
The controversy on the ancestral arsenite oxidizing enzyme; deducing evolutionary histories with phylogeny and thermodynamics.
| العنوان: | The controversy on the ancestral arsenite oxidizing enzyme; deducing evolutionary histories with phylogeny and thermodynamics. |
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| المؤلفون: | Szyttenholm J; Aix-Marseille Univ., CNRS, BIP UMR 7281, FR 3479, IMM, 13402 Marseille Cedex 20, France., Chaspoul F; Aix Marseille Univ., CNRS, IRD, IMBE UMR 7263, Faculté de Pharmacie, 13005 Marseille, France., Bauzan M; Aix-Marseille Univ., CNRS, Plateforme Fermentation, FR3479, IMM, 13402 Marseille Cedex 20, France., Ducluzeau AL; Institute of Arctic Biology, University of Alaska Fairbanks, Fairbanks, AK 99775-7220, USA., Chehade MH; Univ. Grenoble Alpes, CNRS, Grenoble INP, TIMC-IMAG, 38000 Grenoble, France., Pierrel F; Univ. Grenoble Alpes, CNRS, Grenoble INP, TIMC-IMAG, 38000 Grenoble, France., Denis Y; Aix-Marseille Univ., CNRS, Plateforme Transcriptomique, FR3479, IMM, 13402 Marseille Cedex 20, France., Nitschke W; Aix-Marseille Univ., CNRS, BIP UMR 7281, FR 3479, IMM, 13402 Marseille Cedex 20, France., Schoepp-Cothenet B; Aix-Marseille Univ., CNRS, BIP UMR 7281, FR 3479, IMM, 13402 Marseille Cedex 20, France. Electronic address: schoepp@imm.cnrs.fr. |
| المصدر: | Biochimica et biophysica acta. Bioenergetics [Biochim Biophys Acta Bioenerg] 2020 Oct 01; Vol. 1861 (10), pp. 148252. Date of Electronic Publication: 2020 Jun 20. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101731706 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-2650 (Electronic) Linking ISSN: 00052728 NLM ISO Abbreviation: Biochim Biophys Acta Bioenerg Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam : Elsevier |
| مواضيع طبية MeSH: | Evolution, Molecular* , Phylogeny*, Arsenate Reductases/*metabolism , Arsenites/*metabolism , Oxidoreductases/*metabolism, Arsenate Reductases/genetics ; Genomics ; Oxidation-Reduction ; Oxidoreductases/genetics ; Thermodynamics |
| مستخلص: | The three presently known enzymes responsible for arsenic-using bioenergetic processes are arsenite oxidase (Aio), arsenate reductase (Arr) and alternative arsenite oxidase (Arx), all of which are molybdoenzymes from the vast group referred to as the Mo/W-bisPGD enzyme superfamily. Since arsenite is present in substantial amounts in hydrothermal environments, frequently considered as vestiges of primordial biochemistry, arsenite-based bioenergetics has long been predicted to be ancient. Conflicting scenarios, however, have been put forward proposing either Arr/Arx or Aio as operating in the ancestral metabolism. Phylogenetic data argue in favor of Aio whereas biochemical and physiological data led several authors to propose Arx/Arr as the most ancient anaerobic arsenite metabolizing enzymes. Here we combine phylogenetic approaches with physiological and biochemical experiments to demonstrate that the Arx/Arr enzymes could not have been functional in the Archaean geological eon. We propose that Arr reacts with menaquinones to reduce arsenate whereas Arx reacts with ubiquinone to oxidize arsenite, in line with thermodynamic considerations. The distribution of the quinone biosynthesis pathways, however, clearly indicates that the ubiquinone pathway is recent. An updated phylogeny of Arx furthermore reinforces the hypothesis of a recent emergence of this enzyme. We therefore conclude that anaerobic arsenite redox conversion in the Archaean must have been performed in a metabolism involving Aio. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2020. Published by Elsevier B.V.) |
| فهرسة مساهمة: | Keywords: Arsenic; Arsenite oxidation evolutionary history; Phylogeny; Prokaryotes; Quinones; Thermodynamics |
| المشرفين على المادة: | 0 (Arsenites) EC 1.- (Oxidoreductases) EC 1.16.- (arsenite oxidase) EC 1.20.- (Arsenate Reductases) N5509X556J (arsenite) |
| تواريخ الأحداث: | Date Created: 20200623 Date Completed: 20201026 Latest Revision: 20201026 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.bbabio.2020.148252 |
| PMID: | 32569664 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-2650 |
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| DOI: | 10.1016/j.bbabio.2020.148252 |