دورية أكاديمية
أعرض في EDS

The combination of Brazilian red propolis and recombinant protein rCP01850 in the immunoprophylaxis of Corynebacterium pseudotuberculosis infection in mice.

التفاصيل البيبلوغرافية
العنوان: The combination of Brazilian red propolis and recombinant protein rCP01850 in the immunoprophylaxis of Corynebacterium pseudotuberculosis infection in mice.
المؤلفون: Brilhante Bezerra FS; Universidade Federal de Pelotas (UFPel), Campus Capão Do Leão, Centro de Desenvolvimento Tecnológico (CDTec), Capão Do Leão, Brazil; Universidade Federal Rural Do Semi-Árido (UFERSA), Centro de Ciências Biológicas e da Saúde (CCBS), Mossoró, Brazil., Silva Rezende AF; Universidade Federal de Pelotas (UFPel), Campus Capão Do Leão, Centro de Desenvolvimento Tecnológico (CDTec), Capão Do Leão, Brazil., Oliveira Silva MT; Universidade Federal de Pelotas (UFPel), Campus Capão Do Leão, Centro de Desenvolvimento Tecnológico (CDTec), Capão Do Leão, Brazil., Sena-Lopes Â; Universidade Federal de Pelotas (UFPel), Campus Capão Do Leão, Centro de Desenvolvimento Tecnológico (CDTec), Capão Do Leão, Brazil., Roesch-Ely M; Universidade de Caxias Do Sul, Departamento de Tecnologia, Instituto de Biotecnologia, Caxias Do Sul, Brazil., Pêgas Henriques JA; Universidade de Caxias Do Sul, Departamento de Tecnologia, Instituto de Biotecnologia, Caxias Do Sul, Brazil., Padilha FF; Universidade de Tiradentes, Instituto de Tecnologia e Pesquisa (ITP), Aracaju, Brazil., Carvalho Azevedo VA; Universidade Federal de Minas Gerais (UFMG), Instituto de Ciências Biológicas, Belo Horizonte, Brazil., Dias Portela RW; Universidade Federal da Bahia (UFBA), Instituto de Ciências da Saúde, Salvador, Brazil., Seixas FK; Universidade Federal de Pelotas (UFPel), Campus Capão Do Leão, Centro de Desenvolvimento Tecnológico (CDTec), Capão Do Leão, Brazil., Collares TV; Universidade Federal de Pelotas (UFPel), Campus Capão Do Leão, Centro de Desenvolvimento Tecnológico (CDTec), Capão Do Leão, Brazil., Savegnago L; Universidade Federal de Pelotas (UFPel), Campus Capão Do Leão, Centro de Desenvolvimento Tecnológico (CDTec), Capão Do Leão, Brazil., Borsuk S; Universidade Federal de Pelotas (UFPel), Campus Capão Do Leão, Centro de Desenvolvimento Tecnológico (CDTec), Capão Do Leão, Brazil. Electronic address: sibeleborsuk@gmail.com.
المصدر: Microbial pathogenesis [Microb Pathog] 2020 Dec; Vol. 149, pp. 104354. Date of Electronic Publication: 2020 Jun 20.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Academic Press Country of Publication: England NLM ID: 8606191 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-1208 (Electronic) Linking ISSN: 08824010 NLM ISO Abbreviation: Microb Pathog Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London ; Orlando : Academic Press, c1986-
مواضيع طبية MeSH: Corynebacterium Infections* , Corynebacterium pseudotuberculosis* , Lymphadenitis* , Propolis*, Animals ; Brazil ; Mice ; Mice, Inbred BALB C ; Recombinant Proteins/genetics
مستخلص: The immunomodulatory properties of Brazilian red propolis (BRP) have been already described. Also, propolis have been proved to have antibacterial activity on Corynebacterium pseudotuberculosis. An adjuvant effect of red propolis oil was able to induce a significant anti-C. pseudotuberculosis humoral immune response. Here, we demonstrate for the first time the immunostimulant property of BRP hydroalcoholic extract (BRPHE) in a recombinant vaccine against caseous lymphadenitis. Mice BALB/c were allocated in three groups inoculated with: sterile saline solution (G1); BRPHE (G2); or BRPHE combined with the C. pseudotuberculosis rCP01850 recombinant protein (G3) in two doses within a 21-days-interval. Blood samples were collected for the total IgG, IgG1 and IgG2a measurement. Mice were challenged with a virulent C. pseudotuberculosis strain, and other 6 mice were used for IFN-γ and IL-10 levels determination after splenocyte stimulation with the recombinant antigen. G3 showed higher significant levels of antibodies on the 42nd experimental day, with a high IgG2a/IgG1 proportion. G2 and G3 presented significant production of IFN-γ and IL-10, while G3 presented the higher levels of IFN-γ (p < 0.05). After challenge, G2 showed a survival rate of 20%, while 70% of mice from G3 survived the experimental challenge. In conclusion, BRPHE used alone has immunostimulant properties specially on cellular immune response, and when used in combination with the recombinant protein rCP01850 induces cellular and humoral immune responses as well as a significant survival of inoculated mice.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: Adjuvant; Caseous lymphadenitis; Natural products; Vaccine trials
المشرفين على المادة: 0 (Recombinant Proteins)
9009-62-5 (Propolis)
تواريخ الأحداث: Date Created: 20200623 Date Completed: 20210617 Latest Revision: 20210617
رمز التحديث: 20240628
DOI: 10.1016/j.micpath.2020.104354
PMID: 32569789
قاعدة البيانات: MEDLINE
الوصف
تدمد:1096-1208
DOI:10.1016/j.micpath.2020.104354