دورية أكاديمية
MicroRNA-448/EPHA7 axis regulates cell proliferation, invasion and migration via regulation of PI3K/AKT signaling pathway and epithelial-to-mesenchymal transition in non-small cell lung cancer.
| العنوان: | MicroRNA-448/EPHA7 axis regulates cell proliferation, invasion and migration via regulation of PI3K/AKT signaling pathway and epithelial-to-mesenchymal transition in non-small cell lung cancer. |
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| المؤلفون: | Liu HY; Thoracic Surgery, The Second Affiliated Hospital of Shandong First Medical University, Tai'an, China. tyfymys@163.com., Chang J, Li GD, Zhang ZH, Tian J, Mu YS |
| المصدر: | European review for medical and pharmacological sciences [Eur Rev Med Pharmacol Sci] 2020 Jun; Vol. 24 (11), pp. 6139-6149. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Verduci Country of Publication: Italy NLM ID: 9717360 Publication Model: Print Cited Medium: Internet ISSN: 2284-0729 (Electronic) Linking ISSN: 11283602 NLM ISO Abbreviation: Eur Rev Med Pharmacol Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Rome : Verduci, [1997- |
| مواضيع طبية MeSH: | Cell Movement* , Epithelial-Mesenchymal Transition*, Carcinoma, Non-Small-Cell Lung/*metabolism , Lung Neoplasms/*metabolism , MicroRNAs/*metabolism , Phosphatidylinositol 3-Kinases/*metabolism , Proto-Oncogene Proteins c-akt/*metabolism , Receptor, EphA7/*metabolism, Animals ; Carcinoma, Non-Small-Cell Lung/pathology ; Cell Line ; Cell Proliferation ; Humans ; Lung Neoplasms/pathology ; Mice ; MicroRNAs/genetics ; Neoplasms, Experimental/metabolism ; Neoplasms, Experimental/pathology ; Receptor, EphA7/genetics ; Signal Transduction |
| مستخلص: | Objective: Non-small cell lung cancer (NSCLC) is a primary subtype of lung cancers which has a high morbidity and poor prognosis. Emerging evidence has demonstrated that aberrantly expressed microRNAs (miRNAs) were implicated in the regulatory functions of multiple processes during tumorigenesis. In the current study, we explored the functional roles and underlying mechanisms of miR-448 in NSCLC. Patients and Methods: Quantitative real-time polymerase chain reaction assays were conducted to measure miR-448 expressions in 51 pairs of NSCLC tissues and corresponding normal tissues. Moreover, the relationship between miR-448 expressions and clinicopathological characteristics of NSCLC patients was also determined. We then performed transwell assays to explore the functions of miR-448 in NSCLC cell invasion and migration. As we had identified EPHA7 as a functional target of miR-448 in NSCLC cells, the clinical significance of EPHA7 in NSCLC patients was further investigated. Finally, we detected the influence of miR-448 on tumor growth rate and tumor size of NSCLC using tumor xenografts. Results: Underexpressed miR-448 was identified in NSCLC, and low miR-448 expression was confirmed to be associated with the poor prognosis and adverse clinicopathologic features of NSCLC patients. Moreover, functional assays demonstrated that miR-448 overexpression suppressed NSCLC cell proliferation, invasion and migration. EPHA7 was identified as a direct target of miR-448. Additionally, miR-448 restoration suppressed in vivo NSCLC cell growth. Finally, our studies also indicated that miR-448 exerted anti-NSCLC functions via regulating PI3K/AKT signaling pathway and EMT. Conclusions: These results showed that miR-448/EPHA7 axis maybe one of the useful diagnostic and prognostic biomarkers for NSCLC patients. |
| المشرفين على المادة: | 0 (MIRN448 microRNA, human) 0 (MicroRNAs) EC 2.7.10.1 (Receptor, EphA7) EC 2.7.11.1 (Proto-Oncogene Proteins c-akt) |
| تواريخ الأحداث: | Date Created: 20200624 Date Completed: 20210421 Latest Revision: 20210421 |
| رمز التحديث: | 20231215 |
| DOI: | 10.26355/eurrev_202006_21509 |
| PMID: | 32572879 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2284-0729 |
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| DOI: | 10.26355/eurrev_202006_21509 |