التفاصيل البيبلوغرافية
| العنوان: |
Plasma from recovered COVID19 subjects inhibits spike protein binding to ACE2 in a microsphere-based inhibition assay. |
| المؤلفون: |
Gniffke EP, Harrington WE, Dambrauskas N, Jiang Y, Trakhimets O, Vigdorovich V, Frenkel L, Sather DN, Smith SEP |
| المصدر: |
MedRxiv : the preprint server for health sciences [medRxiv] 2020 Jun 11. Date of Electronic Publication: 2020 Jun 11. |
| نوع المنشور: |
Preprint |
| اللغة: |
English |
| بيانات الدورية: |
Country of Publication: United States NLM ID: 101767986 Publication Model: Electronic Cited Medium: Internet NLM ISO Abbreviation: medRxiv Subsets: PubMed not MEDLINE |
| مستخلص: |
High throughput serological tests that can establish the presence and functional activity of anti-SARS-COV2 antibodies are urgently needed. Here we present microsphere-based Flow Cytometry assays that quantify both anti-spike IgGs in plasma, and the ability of plasma to inhibit the binding of spike protein to angiotensin converting enzyme 2 (ACE2). First, we detected anti-trimer IgGs in 22/24 and anti-RBD IgGs in 21/24 COVID+ subjects at a median of 36 (range 14-73) days following documented SARS-CoV-2 RNA (+) secretions. Next, we find that plasma from all 22/24 subjects with anti-trimer IgGs inhibited ACE2-trimer binding to a greater degree than controls, and that the degree of inhibition correlated with anti-trimer IgG levels. Depletion of trimer-reactive Igs from plasma reduced ACE2-trimer inhibitory capacity to a greater degree than depletion of RBD-reactive Igs, suggesting that inhibitory antibodies act by binding both within and outside of the RBD. Amongst the 24 subjects, presence of fever was associated with higher levels of anti-trimer IgG and inhibition of binding to human ACE2. This inhibition assay may be broadly useful to quantify the functional antibody response of recovered COVID19 patients or vaccine recipients in a cell-free assay system. |
| التعليقات: |
Update in: J Infect Dis. 2020 Aug 15;:. (PMID: 32798222) |
| معلومات مُعتمدة: |
K08 AI135072 United States AI NIAID NIH HHS; R01 AI140951 United States AI NIAID NIH HHS; UL1 TR002319 United States TR NCATS NIH HHS |
| تواريخ الأحداث: |
Date Created: 20200625 Latest Revision: 20201013 |
| رمز التحديث: |
20221213 |
| مُعرف محوري في PubMed: |
PMC7302223 |
| DOI: |
10.1101/2020.06.09.20127050 |
| PMID: |
32577669 |
| قاعدة البيانات: |
MEDLINE |
