دورية أكاديمية
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Microarray Analysis of Gene Expression Involved in Butyrate-Resistant Colorectal Carcinoma HCT116 Cells.

التفاصيل البيبلوغرافية
العنوان: Microarray Analysis of Gene Expression Involved in Butyrate-Resistant Colorectal Carcinoma HCT116 Cells.
المؤلفون: Khonthun C; Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand., Saikachain N; Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand., Popluechai S; School of Science, Mae Fah Luang University, Chaiang Rai, Thailand.; Gut microbiome research group, Mae Fah Luang University, Chaiang Rai, Thailand., Kespechara K; Bangkok Hospital Pattaya, Chonburi, Thailand., Hiranyakas A; Bangkok Hospital Phuket, Phuket, Thailand., Srikummool M; Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand., Surangkul D; Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand.
المصدر: Asian Pacific journal of cancer prevention : APJCP [Asian Pac J Cancer Prev] 2020 Jun 01; Vol. 21 (6), pp. 1739-1746. Date of Electronic Publication: 2020 Jun 01.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Asian Pacific Organization for Cancer Prevention Country of Publication: Thailand NLM ID: 101130625 Publication Model: Electronic Cited Medium: Internet ISSN: 2476-762X (Electronic) Linking ISSN: 15137368 NLM ISO Abbreviation: Asian Pac J Cancer Prev Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Bangkok : Asian Pacific Organization for Cancer Prevention,
مواضيع طبية MeSH: Biomarkers, Tumor/*genetics , Butyrates/*pharmacology , Colorectal Neoplasms/*genetics , Drug Resistance, Neoplasm/*genetics , Gene Expression Regulation, Neoplastic/*drug effects , Microarray Analysis/*methods , Neoplastic Stem Cells/*metabolism, Colorectal Neoplasms/drug therapy ; Colorectal Neoplasms/pathology ; Gene Expression Profiling ; Humans ; Neoplastic Stem Cells/drug effects ; Neoplastic Stem Cells/pathology ; Tumor Cells, Cultured
مستخلص: Background: Resistance to chemotherapeutic agents is usually found in cancer stem cells (CSCs) and cancer stem-like cells that are often regarded as the target for cancer monitoring. However, the different patterns of their transcriptomic profiling is still unclear.
Objective: This study aims to illustrate the transcriptomic profile of CSCs and butyrate-resistant colorectal carcinoma cells (BR-CRCs), by comparing them with parental colorectal cancer (CRC) cells in order to identify distinguishing transcription patterns of the CSCs and BR-CRCs.
Methods: Parental CRC cells HCT116 (HCT116-PT) were cultured and induced to establish the butyrate resistant cell model (HCT116-BR). Commercial enriching of the HCT116-CSCs were grown in a tumorsphere suspension culture, which was followed firstly by the assessment of butyrate tolerance using MTT and PrestoBlue. Then their gene expression profiling was analyzed by microarray.
Results: The results showed that both butyrate-resistant HCT116 cells (HCT116-BR) and HCT116-CSCs were more tolerant a butyrate effects than HCT116-PT cells. Differentially expressed gene profiles exhibited that IFI27, FOXQ1, PRF1, and SLC2A3 genes were increasingly expressed in CSCs, and were dramatically overexpressed in HCT116-BR cells when compared with HCT116-PT cells. Moreover, PKIB and LOC399959 were downregulated both in HCT116-CSCs and HCT116-BR cells.
Conclusion: Our findings shed light on the transcriptomic profiles of chemoresistant CRC cells. This data should be useful for further study to provide guidelines for clinical prognosis to determine the guidelines for CRC treatment, especially in patients with chemoresistance and designing novel anti-neoplastic agents.
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فهرسة مساهمة: Keywords: Cancer stem cells; Chemoresistance; butyrate; colorectal cancer
المشرفين على المادة: 0 (Biomarkers, Tumor)
0 (Butyrates)
تواريخ الأحداث: Date Created: 20200628 Date Completed: 20210211 Latest Revision: 20240329
رمز التحديث: 20240329
مُعرف محوري في PubMed: PMC7568904
DOI: 10.31557/APJCP.2020.21.6.1739
PMID: 32592372
قاعدة البيانات: MEDLINE
الوصف
تدمد:2476-762X
DOI:10.31557/APJCP.2020.21.6.1739