دورية أكاديمية
أعرض في EDS

3D profilometry and cell viability studies for drug response screening.

التفاصيل البيبلوغرافية
العنوان: 3D profilometry and cell viability studies for drug response screening.
المؤلفون: Morán MC; Departament de Bioquímica i Fisiologia, Secció de Fisiologia - Facultat de Farmàcia i Ciències de l'Alimentació, Universitat de Barcelona, Avda. Joan XXIII, 27-31, 08028 Barcelona, Spain; Institut de Nanociència i Nanotecnologia - IN(2)UB, Universitat de Barcelona, Avda. Diagonal, 645, 08028 Barcelona, Spain. Electronic address: mcmoranb@ub.edu., Cirisano F; CNR-ICMATE Istituto di Chimica della Materia Condensata e di Tecnologie per l'Energia, via De Marini, 6, 16149 Genova, Italy., Ferrari M; Institut de Nanociència i Nanotecnologia - IN(2)UB, Universitat de Barcelona, Avda. Diagonal, 645, 08028 Barcelona, Spain; CNR-ICMATE Istituto di Chimica della Materia Condensata e di Tecnologie per l'Energia, via De Marini, 6, 16149 Genova, Italy. Electronic address: michele.ferrari@ge.icmate.cnr.it.
المصدر: Materials science & engineering. C, Materials for biological applications [Mater Sci Eng C Mater Biol Appl] 2020 Oct; Vol. 115, pp. 111142. Date of Electronic Publication: 2020 May 30.
نوع المنشور: Comparative Study; Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101484109 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-0191 (Electronic) Linking ISSN: 09284931 NLM ISO Abbreviation: Mater Sci Eng C Mater Biol Appl Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam : Elsevier
مواضيع طبية MeSH: Fluorouracil/*pharmacology , HeLa Cells/*cytology , Imaging, Three-Dimensional/*methods , Swiss 3T3 Cells/*cytology, Animals ; Cell Line ; Cell Size/drug effects ; Cell Survival/drug effects ; Fluorouracil/chemistry ; HeLa Cells/drug effects ; Humans ; Mice ; Nanoparticles ; Swiss 3T3 Cells/drug effects
مستخلص: In vitro tests for assessing cell viability and drug response are widely employed for determining cytotoxicity of drugs, chemicals, or material substrates. These assays have some advantages, such as speed, reduced cost, and potential for automation. However, since these tests are often run with a huge amount of cells, the characteristic properties of a single cell can be masked leading to a lack of the diagnostic features of these assays. Vital processes as proliferation and cell death (either necrosis or apoptosis) are associated to drastic changes of volume and surface analysis techniques like 3D optical scanning profilometry allow noninvasive and nondestructive approach with fast detection and good resolution at nano-microscale. Here, we demonstrate how coupling noninvasive morphological surface analysis techniques with well assessed biochemical methods can help to establish the relationship between the modifications on cellular viability induced by precursors of proliferation and cell death and variations on cell volume induced by these treatments. The proposed approach has demonstrated improved efficiency on the assessment of inductive changes on tumoral cells in comparison to non-tumoral cells upon administration of proliferative nontoxic or cytotoxic substances like chemotherapeutics.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: 3D profilometry; Apoptosis; Cell viability; Cell volume; Drug screening; Proliferation
المشرفين على المادة: U3P01618RT (Fluorouracil)
تواريخ الأحداث: Date Created: 20200701 Date Completed: 20210329 Latest Revision: 20210329
رمز التحديث: 20240628
DOI: 10.1016/j.msec.2020.111142
PMID: 32600730
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-0191
DOI:10.1016/j.msec.2020.111142