دورية أكاديمية
أعرض في EDS

Biological properties of almond proteins produced by aqueous and enzyme-assisted aqueous extraction processes from almond cake.

التفاصيل البيبلوغرافية
العنوان: Biological properties of almond proteins produced by aqueous and enzyme-assisted aqueous extraction processes from almond cake.
المؤلفون: de Souza TSP; Department of Food Science and Technology, University of California, Davis, One Shields Avenue, Davis, CA, 95616, USA.; Department of Food Science, Food and Nutrition Graduate Program, Federal University of the State of Rio de Janeiro, 296, Pasteur Avenue, Urca, Rio de Janeiro, RJ, 29622290-240, Brazil., Dias FFG; Department of Food Science and Technology, University of California, Davis, One Shields Avenue, Davis, CA, 95616, USA., Oliveira JPS; Department of Food Science, Food and Nutrition Graduate Program, Federal University of the State of Rio de Janeiro, 296, Pasteur Avenue, Urca, Rio de Janeiro, RJ, 29622290-240, Brazil., de Moura Bell JMLN; Department of Food Science and Technology, University of California, Davis, One Shields Avenue, Davis, CA, 95616, USA.; Biological and Agricultural Engineering, University of California, Davis, One Shields Avenue, Davis, CA, 95616, USA., Koblitz MGB; Department of Food Science, Food and Nutrition Graduate Program, Federal University of the State of Rio de Janeiro, 296, Pasteur Avenue, Urca, Rio de Janeiro, RJ, 29622290-240, Brazil. maria.koblitz@unirio.br.
المصدر: Scientific reports [Sci Rep] 2020 Jul 02; Vol. 10 (1), pp. 10873. Date of Electronic Publication: 2020 Jul 02.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Antioxidants/*pharmacology , Glycoside Hydrolase Inhibitors/*pharmacology , Plant Extracts/*pharmacology , Plant Proteins/*pharmacology , Prunus dulcis/*metabolism , alpha-Glucosidases/*chemistry, Metabolic Syndrome/prevention & control ; Prunus dulcis/chemistry
مستخلص: The almond cake is a protein-rich residue generated by the mechanical expression of the almond oil. The effects of the aqueous (AEP) and enzyme-assisted aqueous extraction processes (EAEP) on the biological properties of the almond cake protein were evaluated. Total phenolic content (TPC), antioxidant capacity, inhibitory effects against crucial enzymes related to metabolic syndrome, antimicrobial potential, and in vitro protein digestibility profile were assessed. EAEP provided the best results for antioxidant capacity by both ORAC (397.2 µmol TE per g) and ABTS (650.5 µmol TE per g) methods and also showed a high (~ 98%) potential for α-glucosidase inhibition. The AEP resulted in protein extracts with the highest lipase inhibition (~ 70%) in a dose-dependent way. Enzymatic kinetic analyses revealed that EAEP generated uncompetitive inhibitors against α-glucosidase, while EAEP, AEP, and HEX-AEP (used as control) generated the same kind of inhibitors against lipase. No protein extract was effective against any of the bacteria strains tested at antimicrobial assays. An in silico theoretical hydrolysis of amandin subunits corroborated with the results found for antioxidant capacity, enzyme inhibitory experiments, and antimicrobial activity. Digestibility results indicated that the digestive proteases used were efficient in hydrolyzing almond proteins, regardless of the extraction applied and that HEX-AEP presented the highest digestibility (85%). In summary, EAEP and AEP skim proteins have the potential to be used as a nutraceutical ingredient. The biological properties observed in these extracts could help mitigate the development of metabolic syndrome where EAEP and AEP skim proteins could be potentially used as a prophylactic therapy for diabetes and obesity, respectively.
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المشرفين على المادة: 0 (Antioxidants)
0 (Glycoside Hydrolase Inhibitors)
0 (Plant Extracts)
0 (Plant Proteins)
EC 3.2.1.20 (alpha-Glucosidases)
تواريخ الأحداث: Date Created: 20200704 Date Completed: 20201215 Latest Revision: 20240329
رمز التحديث: 20240329
مُعرف محوري في PubMed: PMC7331752
DOI: 10.1038/s41598-020-67682-3
PMID: 32616827
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-020-67682-3