دورية أكاديمية
أعرض في EDS

Rapid activation of distinct members of multigene families in Plasmodium spp.

التفاصيل البيبلوغرافية
العنوان: Rapid activation of distinct members of multigene families in Plasmodium spp.
المؤلفون: Omelianczyk RI; School of Biological Sciences, Nanyang Technological University, Singapore, Singapore., Loh HP; School of Biological Sciences, Nanyang Technological University, Singapore, Singapore., Chew M; School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.; Singapore-MIT Alliance for Research and Technology, Infectious Disease Interdisciplinary Research Group, Singapore, Singapore., Hoo R; School of Biological Sciences, Nanyang Technological University, Singapore, Singapore., Baumgarten S; Biology of Host-Parasite Interactions Unit, Department of Parasites and Insect Vectors, Institut Pasteur, CNRS ERL 9195, INSERM Unit U1201, Paris, France., Renia L; Singapore Immunology Network (SIgN), A*STAR, Biopolis, Singapore, Singapore., Chen J; Singapore-MIT Alliance for Research and Technology, Infectious Disease Interdisciplinary Research Group, Singapore, Singapore.; Koch Institute for Integrative Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA., Preiser PR; School of Biological Sciences, Nanyang Technological University, Singapore, Singapore. prpreiser@ntu.edu.sg.; Singapore-MIT Alliance for Research and Technology, Infectious Disease Interdisciplinary Research Group, Singapore, Singapore. prpreiser@ntu.edu.sg.
المصدر: Communications biology [Commun Biol] 2020 Jul 03; Vol. 3 (1), pp. 351. Date of Electronic Publication: 2020 Jul 03.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group UK Country of Publication: England NLM ID: 101719179 Publication Model: Electronic Cited Medium: Internet ISSN: 2399-3642 (Electronic) Linking ISSN: 23993642 NLM ISO Abbreviation: Commun Biol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London, United Kingdom : Nature Publishing Group UK, [2018]-
مواضيع طبية MeSH: Gene Expression Regulation* , Multigene Family*, Erythrocytes/*metabolism , Malaria, Falciparum/*genetics , Plasmodium falciparum/*genetics , Protozoan Proteins/*metabolism, Animals ; Erythrocytes/parasitology ; Humans ; Malaria, Falciparum/parasitology ; Mice ; Protozoan Proteins/genetics
مستخلص: The genomes of Plasmodium spp. encode a number of different multigene families that are thought to play a critical role for survival. However, with the exception of the P. falciparum var genes, very little is known about the biological roles of any of the other multigene families. Using the recently developed Selection Linked Integration method, we have been able to activate the expression of a single member of a multigene family of our choice in Plasmodium spp. from its endogenous promoter. We demonstrate the usefulness of this approach by activating the expression of a unique var, rifin and stevor in P. falciparum as well as yir in P. yoelii. Characterization of the selected parasites reveals differences between the different families in terms of mutual exclusive control, co-regulation, and host adaptation. Our results further support the application of the approach for the study of multigene families in Plasmodium and other organisms.
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المشرفين على المادة: 0 (Protozoan Proteins)
تواريخ الأحداث: Date Created: 20200705 Date Completed: 20210615 Latest Revision: 20210703
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC7334209
DOI: 10.1038/s42003-020-1081-3
PMID: 32620892
قاعدة البيانات: MEDLINE
الوصف
تدمد:2399-3642
DOI:10.1038/s42003-020-1081-3