التفاصيل البيبلوغرافية
| العنوان: |
The Immunoprobe Aggregation State is Central to Dipstick Immunoassay Performance. |
| المؤلفون: |
Hristov DR, Pimentel AJ, Ujialele G, Hamad-Schifferli K |
| المصدر: |
ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2020 Aug 05; Vol. 12 (31), pp. 34620-34629. Date of Electronic Publication: 2020 Jul 21. |
| نوع المنشور: |
Journal Article |
| اللغة: |
English |
| بيانات الدورية: |
Publisher: American Chemical Society Country of Publication: United States NLM ID: 101504991 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1944-8252 (Electronic) Linking ISSN: 19448244 NLM ISO Abbreviation: ACS Appl Mater Interfaces Subsets: MEDLINE |
| أسماء مطبوعة: |
Original Publication: Washington, D.C. : American Chemical Society |
| مواضيع طبية MeSH: |
Immunoassay*, Antibodies/*chemistry , Nanoparticles/*chemistry, Paper ; Particle Size ; Surface Properties |
| مستخلص: |
As new infectious disease outbreaks become more likely, it is important to be able to develop and deploy appropriate testing in time. Paper-based immunoassays are rapid, cheap, and easy to produce at scale and relatively user friendly but often suffer from low selectivity and sensitivity. Understanding the molecular mechanisms of paper immunoassays may help improve and hasten development and therefore production and market availability. Here, we study how the behavior of nanoparticle-antibody immunoprobes in paper dipstick immunoassays is impacted by synthesis strategy and surface chemistry architecture. We conjugate gold nanoparticles to polyclonal anti-immunoglobulin G (IgG) and anti-zika NS1 antibodies by electrostatic adsorption and N -hydroxysuccinimide (NHS) and hydrazide (Hz) chemistries. The immunoprobes were used in paper immunoassays and the effective affinity for the antigen was quantified from the test line intensities, as well as the distribution of the immunoprobes throughout the strips. The results show that nanoparticle colloidal stability, both post synthesis and during antigen binding, is a key factor and affects immunoassay results and performance, often through reduction or loss of signal. |
| فهرسة مساهمة: |
Keywords: aggregation; nanoparticles; nanoparticle−antibody conjugates; paper-based diagnostics; sandwich immunoassay |
| المشرفين على المادة: |
0 (Antibodies) |
| تواريخ الأحداث: |
Date Created: 20200708 Date Completed: 20210216 Latest Revision: 20210216 |
| رمز التحديث: |
20240628 |
| DOI: |
10.1021/acsami.0c08628 |
| PMID: |
32633115 |
| قاعدة البيانات: |
MEDLINE |
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