دورية أكاديمية
FOXP3 Genetic Variants Do Not Impact Circulating and Cervical Interleukin-10 Levels in Human Papillomavirus Infection in Women.
| العنوان: | FOXP3 Genetic Variants Do Not Impact Circulating and Cervical Interleukin-10 Levels in Human Papillomavirus Infection in Women. |
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| المؤلفون: | Cezar-Dos-Santos F; Laboratory of Molecular Genetics and Immunology, Department of Pathological Sciences, Biological Sciences Center, Londrina State University, Londrina, Paraná, Brazil., Berti FCB; Laboratory of Human Molecular Genetics, Department of Genetics, Federal University of Paraná, Curitiba, PR, Brazil., Okuyama NCM; Laboratory of Molecular Genetics and Immunology, Department of Pathological Sciences, Biological Sciences Center, Londrina State University, Londrina, Paraná, Brazil., Trugilo KP; Laboratory of Molecular Genetics and Immunology, Department of Pathological Sciences, Biological Sciences Center, Londrina State University, Londrina, Paraná, Brazil., Simão ANC; Research Laboratory in Applied Immunology, Department of Pathology, Clinical Analysis and Toxicology, Health Sciences Center, Londrina State University, Londrina, Paraná, Brazil., Watanabe MAE; Laboratory of Studies and Applications of DNA Polymorphisms and Immunology, Department of Pathological Sciences, Biological Sciences Center, Londrina State University, Londrina, Paraná, Brazil., Brajão de Oliveira K; Laboratory of Molecular Genetics and Immunology, Department of Pathological Sciences, Biological Sciences Center, Londrina State University, Londrina, Paraná, Brazil. |
| المصدر: | Viral immunology [Viral Immunol] 2020 Dec; Vol. 33 (10), pp. 652-655. Date of Electronic Publication: 2020 Jul 06. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 8801552 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1557-8976 (Electronic) Linking ISSN: 08828245 NLM ISO Abbreviation: Viral Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York, NY : Mary Ann Liebert, Inc., c1987- |
| مواضيع طبية MeSH: | Polymorphism, Single Nucleotide*, Forkhead Transcription Factors/*genetics , Interleukin-10/*analysis , Interleukin-10/*blood, Case-Control Studies ; Female ; Forkhead Transcription Factors/classification ; Genetic Predisposition to Disease ; Genotype ; Humans ; T-Lymphocytes, Regulatory/immunology |
| مستخلص: | Regulatory T cell (Treg) lineage plays a central role in inflammation and autoimmunity control. Interleukin-10 (IL-10) has been described as a pleiotropic cytokine that is mainly released by CD4 + CD25 + FOXP3 + Treg cells and has a potent immunosuppressive activity. Forkhead box P3 (FOXP3) transcription factor expression is crucial for Treg to function as a suppressor cell, and FOXP3 gene single nucleotide variants (SNVs) have already been shown to influence on viral pathogenesis. This study was conducted to evaluate the plasmatic and cervical levels of IL-10 in human papillomavirus-infected and uninfected patients and investigate whether the FOXP3 intron -1 SNVs rs3761548 and rs2232365 might alter IL-10 secretion. SNVs were genotyped by the characterization of polymerase chain reaction (PCR) products based on sequence-specific enzymatic cleavage using restriction fragment length polymorphism (RFLP) method. IL-10 levels were determined by quantitative enzyme-linked immunosorbent assay (ELISA). In conclusion, the data indicate that there is no association between FOXP3 SNVs and circulating and cervical IL-10 levels. This finding provides a rationale that IL-10 gene activation is independent of FOXP3 transcription factor activities on Treg cells. |
| فهرسة مساهمة: | Keywords: IL-10; cytokine; forkhead box P3; genetic variants; human papillomavirus |
| المشرفين على المادة: | 0 (FOXP3 protein, human) 0 (Forkhead Transcription Factors) 0 (IL10 protein, human) 130068-27-8 (Interleukin-10) |
| تواريخ الأحداث: | Date Created: 20200708 Date Completed: 20210819 Latest Revision: 20210819 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1089/vim.2020.0040 |
| PMID: | 32634048 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1557-8976 |
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| DOI: | 10.1089/vim.2020.0040 |