دورية أكاديمية
Toll-Like Receptor Ligand Based Adjuvant, PorB, Increases Antigen Deposition on Germinal Center Follicular Dendritic Cells While Enhancing the Follicular Dendritic Cells Network.
| العنوان: | Toll-Like Receptor Ligand Based Adjuvant, PorB, Increases Antigen Deposition on Germinal Center Follicular Dendritic Cells While Enhancing the Follicular Dendritic Cells Network. |
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| المؤلفون: | Lisk C; Section of Infectious Diseases, Department of Medicine, Boston Medical Center, Boston, MA, United States., Yuen R; Department of Microbiology, Boston University School of Medicine, Boston, MA, United States., Kuniholm J; Department of Microbiology, Boston University School of Medicine, Boston, MA, United States., Antos D; Department of Microbiology and Immunology, University of Pittsburgh School of Medicine, Pittsburgh, PA, United States., Reiser ML; DRK-Blutspendedienst, BaWü-Hessen gGmbH, Frankfurt, Germany., Wetzler LM; Section of Infectious Diseases, Department of Medicine, Boston Medical Center, Boston, MA, United States.; Department of Microbiology, Boston University School of Medicine, Boston, MA, United States. |
| المصدر: | Frontiers in immunology [Front Immunol] 2020 Jun 19; Vol. 11, pp. 1254. Date of Electronic Publication: 2020 Jun 19 (Print Publication: 2020). |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Research Foundation] Country of Publication: Switzerland NLM ID: 101560960 Publication Model: eCollection Cited Medium: Internet ISSN: 1664-3224 (Electronic) Linking ISSN: 16643224 NLM ISO Abbreviation: Front Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Lausanne : Frontiers Research Foundation] |
| مواضيع طبية MeSH: | Adjuvants, Immunologic/*pharmacology , Dendritic Cells, Follicular/*immunology , Germinal Center/*immunology , Porins/*immunology, Animals ; B-Lymphocytes/immunology ; Female ; Male ; Mice ; Mice, Inbred C57BL ; Ovalbumin/immunology ; Vaccination ; Vaccines/immunology |
| مستخلص: | Vaccines are arguably one of the greatest advancements in modern medicine. Subunit vaccines comprise the majority of current preparations and consist of two main components-antigen and adjuvant. The antigen is a small molecule against which the vaccine induces an immune response to provide protection via the immunostimulatory ability of the adjuvant. Our laboratory has investigated the adjuvant properties of Toll-like receptor (TLR) ligand-based adjuvants, especially the outer membrane protein from Neisseria mengingitidis , PorB. In this current study we used PorB, along with CpG, an intracellular TLR9 agonist, and a non-TLR adjuvant, aluminum salts (Alum), to further investigate cellular mechanisms of adjuvanticity, focusing on the fate of intact antigen in the germinal center and association with follicular dendritic cells (FDCs). FDCs are located in the B cell light zone of the germinal center and are imperative for affinity maturation. They are stromal cells that retain whole intact antigen allowing recognition by the B cell receptor of the germinal center B cells. Our studies demonstrate that TLR ligands, but not Alum, increase the FDC network, while PorB and Alum increased colocalization of FDC and the model soluble antigen, ovalbumin (OVA). As PorB is the only adjuvant tested that induces both a higher number of FDCs and increased deposition of antigen on FDCs, it has the greatest ability to increase FDC-antigen interaction, essential for induction of B cell affinity maturation. These studies demonstrate a further mechanism and potential superiority of PorB as an adjuvant and its influence on antibody production. (Copyright © 2020 Lisk, Yuen, Kuniholm, Antos, Reiser and Wetzler.) |
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| معلومات مُعتمدة: | R01 AI040944 United States AI NIAID NIH HHS |
| فهرسة مساهمة: | Keywords: PorB; TLR-ligand based adjuvants; TLR2; adjuvants; antigen deposition; dendritic cells; follicular dendritic cells; neisseria |
| المشرفين على المادة: | 0 (Adjuvants, Immunologic) 0 (Porins) 0 (Vaccines) 0 (porin protein, Neisseria) 9006-59-1 (Ovalbumin) |
| تواريخ الأحداث: | Date Created: 20200709 Date Completed: 20210405 Latest Revision: 20220113 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC7318107 |
| DOI: | 10.3389/fimmu.2020.01254 |
| PMID: | 32636846 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1664-3224 |
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| DOI: | 10.3389/fimmu.2020.01254 |