دورية أكاديمية
أعرض في EDS

Mandibular undifferentiated pleomorphic sarcoma: Molecular analysis of a primary cell population.

التفاصيل البيبلوغرافية
العنوان: Mandibular undifferentiated pleomorphic sarcoma: Molecular analysis of a primary cell population.
المؤلفون: Amm HM; Oral and Maxillofacial Surgery, University of Alabama at Birmingham, Birmingham, Alabama, USA., DeVilliers P; Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama, USA., Srivastava AR; Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, Alabama, USA., Diniz MG; Department of Pathology and Oral Surgery and Pathology, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Siegal GP; Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama, USA.; Department of Genetics, University of Alabama at Birmingham, Birmingham, Alabama, USA., MacDougall M; Oral and Maxillofacial Surgery, University of Alabama at Birmingham, Birmingham, Alabama, USA.; Faculty of Dentistry, The University of British Columbia, Vancouver, British Columbia, Canada.
المصدر: Clinical and experimental dental research [Clin Exp Dent Res] 2020 Oct; Vol. 6 (5), pp. 495-505. Date of Electronic Publication: 2020 Jul 11.
نوع المنشور: Case Reports; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Wiley Country of Publication: United States NLM ID: 101692332 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2057-4347 (Electronic) Linking ISSN: 20574347 NLM ISO Abbreviation: Clin Exp Dent Res Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Hoboken : Wiley, [2015]-
مواضيع طبية MeSH: Cell Differentiation*, Biomarkers, Tumor/*analysis , Mandible/*pathology , Sarcoma/*pathology, Adult ; Humans ; Immunohistochemistry ; Male ; Mandible/metabolism ; RNA-Seq ; Sarcoma/genetics ; Sarcoma/metabolism ; Young Adult
مستخلص: Background: Undifferentiated pleomorphic sarcomas are one of the most common subtypes of soft tissue sarcomas. These are aggressive mesenchymal tumors and are devoid of the major known biomarkers except vimentin. Our objective was to establish and characterize a primary cell population from a mandibular UPS specimen.
Methods: The tumor was surgically removed from the right mandible of a 24-year-old male with IRB approved signed consent. Tumor was dissected, cultured ex vivo, and a cell population, MUPS-1, were isolated from outgrowths. Gene and protein expression profiles of both the primary tumor and the derived there from cells were obtained by quantitative RT-PCR and immunohistochemistry and included markers of epithelial, endothelial, and mesenchymal differentiation. To better define potential biomarkers, MUPS-1 cells were additionally characterized by RNA sequencing analysis.
Results: Pathological analysis of primary tumor tissue revealed a sarcoma demonstrating multiple pathways of differentiation simultaneously with myxoid, fibrous, and osseous tissue. The isolated cells had a spindle cell-like morphology, were maintained in culture for greater than 20 passages, and formed colonies in soft agar indicating tumorigenicity. The cells, similar to the primary tumor, were strongly positive for vimentin and moderately expressed alkaline phosphatase. RNA-seq analysis revealed the tumor over-expressed several genes compared to normal tissue, including components of the Notch signaling pathway, NOTCH3 and JAG1.
Conclusions: We have successfully established an undifferentiated pleomorphic sarcoma cell population, which will provide a valuable resource for studying fundamental processes and potentially serving as a platform for exploring therapeutic strategies for sarcomas.
(© 2020 The Authors. Clinical and Experimental Dental Research published by John Wiley & Sons Ltd.)
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معلومات مُعتمدة: K99 DE023826 United States DE NIDCR NIH HHS; R00 DE023826 United States DE NIDCR NIH HHS; T90 DE022736 United States DE NIDCR NIH HHS
فهرسة مساهمة: Keywords: MMPs; NOTCH; oral cancer; primary cells; sarcoma
المشرفين على المادة: 0 (Biomarkers, Tumor)
تواريخ الأحداث: Date Created: 20200712 Date Completed: 20210823 Latest Revision: 20210823
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC7545231
DOI: 10.1002/cre2.301
PMID: 32652895
قاعدة البيانات: MEDLINE
الوصف
تدمد:2057-4347
DOI:10.1002/cre2.301