دورية أكاديمية
Characterization of biological peculiarities of the radioprotective activity of double-stranded RNA isolated from Saccharomyces сerevisiae .
| العنوان: | Characterization of biological peculiarities of the radioprotective activity of double-stranded RNA isolated from Saccharomyces сerevisiae . |
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| المؤلفون: | Ritter GS; Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.; Department of Natural Sciences, Novosibirsk State University, Novosibirsk, Russia., Nikolin VP; Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia., Popova NA; Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.; Department of Natural Sciences, Novosibirsk State University, Novosibirsk, Russia., Proskurina AS; Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia., Kisaretova PE; Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia., Taranov OS; State Research Center of Virology and Biotechnology 'Vector', Koltsovo, Russia., Dubatolova TD; Institute of Molecular and Cellular Biology, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia., Dolgova EV; Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia., Potter EA; Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia., Kirikovich SS; Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia., Efremov YR; Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.; Department of Natural Sciences, Novosibirsk State University, Novosibirsk, Russia., Bayborodin SI; Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.; Department of Natural Sciences, Novosibirsk State University, Novosibirsk, Russia., Romanenko MV; Department of Natural Sciences, Novosibirsk State University, Novosibirsk, Russia., Meschaninova MI; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia., Venyaminova AG; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia., Kolchanov NA; Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia., Shurdov MA; LLC 'Panagen', Gorno-Altaisk, Russia., Bogachev SS; Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia. |
| المصدر: | International journal of radiation biology [Int J Radiat Biol] 2020 Sep; Vol. 96 (9), pp. 1173-1191. Date of Electronic Publication: 2020 Jul 28. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Taylor & Francis Country of Publication: England NLM ID: 8809243 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1362-3095 (Electronic) Linking ISSN: 09553002 NLM ISO Abbreviation: Int J Radiat Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London ; New York : Taylor & Francis, [c1988- |
| مواضيع طبية MeSH: | RNA, Double-Stranded/*pharmacology , RNA, Fungal/*pharmacology , Radiation-Protective Agents/*pharmacology , Saccharomyces cerevisiae/*genetics, Animals ; Dose-Response Relationship, Radiation ; Gamma Rays/adverse effects ; Mice ; Time Factors |
| مستخلص: | The Purpose of the Article: Protection from ionizing radiation is the most important component in the curing malignant neoplasms, servicing atomic reactors, and resolving the situations associated with uncontrolled radioactive pollutions. In this regard, discovering new effective radioprotectors as well as novel principles of protecting living organisms from high-dose radiation is the most important factor, determining the new approaches in medical and technical usage of radiation. Materials and Methods: Experimental animals were irradiated on the γ-emitter (Cs 137 ) with a dose of 9.4 Gy. Radioprotective properties of several agents (total RNA, single-stranded RNA, double-stranded RNA and B-190) were estimated by the survival/death rates of experimental animals within 30-90 d. Pathomorphological examination of internal organs end electron microscope assay was done on days 9-12 after irradiation. Cloning and other molecular procedures were performed accordingly to commonly accepted protocols. For assessment of the internalization of labeled nucleic acid, bone marrow cells were incubated with double-stranded RNA labeled with 6-FAM fluorescent dye. Cells with internalized double-stranded RNA were assayed using Axio Imager M1 microscope. In the other experiment, bone marrow cells after incubation with double-stranded RNA were stained with Cy5-labeled anti-CD34 antibodies and assayed using Axioskop 2 microscope. Results: In this study, several biological features of the radioprotective action of double-stranded RNA are characterized. It was shown that 160 µg of the double-stranded RNA per mouse protect experimental animals from the absolutely lethal dose of γ-radiation of 9.4 Gy. In different experiments, 80-100% of irradiated animals survive and live until their natural death. Radioprotective properties of double-stranded RNA were found to be independent on its sequence, but strictly dependent on its double-stranded form. Moreover, double-stranded RNA must have 'open' ends of the molecule to exert its radioprotective activity. Conclusions: Experiments indicate that radioprotective effect of double-stranded RNA is tightly bound to its internalization into hematopoietic stem cells, which further repopulate the spleen parenchyma of irradiated mice. Actively proliferating progenitors form the splenic colonies, which further serve as the basis for restoration of hematopoiesis and immune function and determine the survival of animals received the lethal dose of radiation. |
| فهرسة مساهمة: | Keywords: B-190; Double-stranded RNA; double-strand breaks; spleen colonies |
| المشرفين على المادة: | 0 (RNA, Double-Stranded) 0 (RNA, Fungal) 0 (Radiation-Protective Agents) |
| تواريخ الأحداث: | Date Created: 20200714 Date Completed: 20210521 Latest Revision: 20210521 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1080/09553002.2020.1793020 |
| PMID: | 32658564 |
| قاعدة البيانات: | MEDLINE |
PDF full text from Publisher | تدمد: | 1362-3095 |
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| DOI: | 10.1080/09553002.2020.1793020 |