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Laboratory colonization by Dirofilaria immitis alters the microbiome of female Aedes aegypti mosquitoes.

التفاصيل البيبلوغرافية
العنوان: Laboratory colonization by Dirofilaria immitis alters the microbiome of female Aedes aegypti mosquitoes.
المؤلفون: Adegoke A; Center for Molecular and Cellular Biosciences, School of Biological, Environmental, and Earth Sciences, The University of Southern Mississippi, Hattiesburg, MS, 39406, USA., Neff E; Savannah River Ecology Laboratory, University of Georgia, P.O. Drawer E, Aiken, SC, 29802, USA.; Warnell School of Forestry and Natural Resources, University of Georgia, Athens, GA, 30602, USA., Geary A; Center for Molecular and Cellular Biosciences, School of Biological, Environmental, and Earth Sciences, The University of Southern Mississippi, Hattiesburg, MS, 39406, USA., Husser MC; Center for Molecular and Cellular Biosciences, School of Biological, Environmental, and Earth Sciences, The University of Southern Mississippi, Hattiesburg, MS, 39406, USA., Wilson K; Center for Molecular and Cellular Biosciences, School of Biological, Environmental, and Earth Sciences, The University of Southern Mississippi, Hattiesburg, MS, 39406, USA., Norris SM; Center for Molecular and Cellular Biosciences, School of Biological, Environmental, and Earth Sciences, The University of Southern Mississippi, Hattiesburg, MS, 39406, USA., Dharmarajan G; Savannah River Ecology Laboratory, University of Georgia, P.O. Drawer E, Aiken, SC, 29802, USA., Karim S; Center for Molecular and Cellular Biosciences, School of Biological, Environmental, and Earth Sciences, The University of Southern Mississippi, Hattiesburg, MS, 39406, USA. Shahid.Karim@usm.edu.
المصدر: Parasites & vectors [Parasit Vectors] 2020 Jul 13; Vol. 13 (1), pp. 349. Date of Electronic Publication: 2020 Jul 13.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101462774 Publication Model: Electronic Cited Medium: Internet ISSN: 1756-3305 (Electronic) Linking ISSN: 17563305 NLM ISO Abbreviation: Parasit Vectors Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central
مواضيع طبية MeSH: Aedes*/microbiology , Aedes*/parasitology , Dirofilaria immitis* , Microbiota*, Animals ; Bacteria/genetics ; Bacteria/isolation & purification ; Cat Diseases/parasitology ; Cat Diseases/transmission ; Cats ; DNA, Bacterial ; Dirofilariasis/transmission ; Dog Diseases/parasitology ; Dog Diseases/transmission ; Dogs ; Female ; Klebsiella oxytoca/genetics ; Klebsiella oxytoca/isolation & purification ; Metagenome ; Metagenomics ; Mosquito Vectors/microbiology ; Mosquito Vectors/parasitology ; Phylogeny ; RNA, Ribosomal, 16S ; Symbiosis
مستخلص: Background: The ability of blood-feeding arthropods to successfully acquire and transmit pathogens of medical and veterinary importance has been shown to be interfered with, or enhanced by, the arthropod's native microbiome. Mosquitoes transmit viruses, protozoan and filarial nematodes, the majority of which contribute to the 17% of infectious disease cases worldwide. Dirofilaria immitis, a mosquito-transmitted filarial nematodes of dogs and cats, is vectored by several mosquito species including Aedes aegypti.
Methods: In this study, we investigated the impact of D. immitis colonization on the microbiome of laboratory reared female Ae. aegypti. Metagenomic analysis of the V3-V4 variable region of the microbial 16S RNA gene was used for identification of the microbial differences down to species level.
Results: We generated a total of 1068 OTUs representing 16 phyla, 181 genera and 271 bacterial species. Overall, in order of abundance, Proteobacteria, Bacteroidetes, Actinobacteria and Firmicutes were the most represented phylum with D. immitis-infected mosquitoes having more of Proteobacteria (71%) than uninfected mosquitoes (56.9%). An interesting finding in this study is the detection of Klebsiella oxytoca in relatively similar abundance in infected and uninfected mosquitoes, suggesting a possible endosymbiotic relationship, and has been previously shown to indirectly compete for nutrients with fungi on domestic housefly eggs and larvae. While D. immitis colonization has no effect on the overall species richness, we identified significant differences in the composition of selected bacterial genera and phyla between the two groups. We also reported distinct compositional and phylogenetic differences in the individual bacterial species when commonly identified bacteria were compared.
Conclusions: To the best of our knowledge, this is the first study to understand the impact of a filarial infection on the microbiome of its mosquito vector. Further studies are required to identify bacteria species that could play an important role in the mosquito biology. While the microbiome composition of Ae. aegypti mosquito have been previously reported, our study shows that in an effort to establish itself, a filarial nematode modifies and alters the overall microbial diversity within its mosquito host.
References: Int J Parasitol Drugs Drug Resist. 2013 Jun 06;3:102-8. (PMID: 24533299)
Am J Trop Med Hyg. 2018 Dec;99(6):1489-1498. (PMID: 30350766)
J Vet Med Sci. 2003 Aug;65(8):945-7. (PMID: 12951432)
Nat Methods. 2016 Jul;13(7):581-3. (PMID: 27214047)
Mol Ecol. 2018 Jan;27(1):297-309. (PMID: 29165845)
Vet Parasitol. 2011 Sep 27;181(2-4):388-92. (PMID: 21570194)
Proc Natl Acad Sci U S A. 2020 Feb 18;117(7):3711-3717. (PMID: 32015105)
Vet Parasitol. 2015 Jun 15;210(3-4):167-78. (PMID: 25936435)
Acta Trop. 2019 Dec;200:105174. (PMID: 31525322)
Vet Clin North Am Small Anim Pract. 2009 Nov;39(6):1127-58, vii. (PMID: 19932367)
J Am Vet Med Assoc. 1970 Nov 15;157(10):1354-9. (PMID: 4394832)
PLoS Negl Trop Dis. 2009 Oct 13;3(10):e529. (PMID: 19823571)
Parasit Vectors. 2018 Jan 17;11(1):39. (PMID: 29343304)
FEMS Microbiol Ecol. 2019 Jan 1;95(1):. (PMID: 30357406)
ISME J. 2010 Jan;4(1):28-37. (PMID: 19617877)
BMC Microbiol. 2018 Oct 4;18(1):126. (PMID: 30286722)
Vet Parasitol. 2005 Oct 24;133(2-3):157-80. (PMID: 16039780)
Infect Genet Evol. 2019 Jan;67:191-209. (PMID: 30465912)
J Med Entomol. 2020 Feb 27;57(2):608-614. (PMID: 31687760)
Curr Opin Insect Sci. 2018 Aug;28:59-65. (PMID: 30551768)
BMC Ecol. 2010 Mar 12;10:8. (PMID: 20226023)
Am J Trop Med Hyg. 1969 May;18(3):411-5. (PMID: 4976739)
Environ Microbiol. 2014 Sep;16(9):2980-94. (PMID: 24428613)
Mol Ecol. 2014 Jun;23(11):2727-39. (PMID: 24766707)
PLoS Negl Trop Dis. 2011 Nov;5(11):e1261. (PMID: 22140585)
PLoS One. 2017 May 5;12(5):e0176573. (PMID: 28475624)
J Insect Sci. 2018 Nov 1;18(6):. (PMID: 30508201)
Curr Biol. 2019 Nov 18;29(22):3946-3952.e5. (PMID: 31679930)
PLoS One. 2011;6(9):e24767. (PMID: 21957459)
Proc Biol Sci. 2008 Feb 22;275(1633):463-71. (PMID: 18077250)
Parasit Vectors. 2011 Jun 14;4:105. (PMID: 21672186)
Naturwissenschaften. 2009 Sep;96(9):1127-32. (PMID: 19636523)
Parasit Vectors. 2016 Oct 10;9(1):540. (PMID: 27724981)
Front Cell Infect Microbiol. 2017 Apr 07;7:114. (PMID: 28439499)
PLoS Negl Trop Dis. 2012;6(3):e1561. (PMID: 22413032)
Microbiome. 2018 Mar 20;6(1):49. (PMID: 29554951)
Curr Opin Insect Sci. 2014 Sep 1;3:6-13. (PMID: 25584199)
PLoS One. 2010 Mar 10;5(3):e9490. (PMID: 20224823)
Science. 2011 May 13;332(6031):855-8. (PMID: 21566196)
Parasit Vectors. 2014 Jun 06;7:264. (PMID: 24906567)
Front Physiol. 2012 Jun 12;3:196. (PMID: 22701433)
Nat Protoc. 2020 Mar;15(3):799-821. (PMID: 31942082)
FEMS Microbiol Rev. 2013 Sep;37(5):699-735. (PMID: 23692388)
Top Companion Anim Med. 2011 Nov;26(4):186-92. (PMID: 22152606)
Front Microbiol. 2016 Sep 21;7:1485. (PMID: 27708633)
Antimicrob Resist Infect Control. 2015 Oct 26;4:42. (PMID: 26504518)
J Biol Chem. 2008 Feb 8;283(6):3217-23. (PMID: 18065421)
Parasit Vectors. 2016 Jun 30;9(1):375. (PMID: 27363842)
Cell Host Microbe. 2019 Jan 9;25(1):101-112.e5. (PMID: 30595552)
Top Companion Anim Med. 2011 Nov;26(4):178-85. (PMID: 22152605)
Nucleic Acids Res. 2017 Jul 3;45(W1):W180-W188. (PMID: 28449106)
Front Cell Infect Microbiol. 2017 Jun 08;7:236. (PMID: 28642842)
Parasit Vectors. 2013 May 20;6:146. (PMID: 23688194)
معلومات مُعتمدة: P20 GM103476 United States GM NIGMS NIH HHS
فهرسة مساهمة: Keywords: Aedes aegypti; Dirofilaria immitis; Dog heartworm; Metagenome; Microbiome; Mosquitoes
المشرفين على المادة: 0 (DNA, Bacterial)
0 (RNA, Ribosomal, 16S)
تواريخ الأحداث: Date Created: 20200715 Date Completed: 20210316 Latest Revision: 20210317
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC7359625
DOI: 10.1186/s13071-020-04218-8
PMID: 32660640
قاعدة البيانات: MEDLINE
الوصف
تدمد:1756-3305
DOI:10.1186/s13071-020-04218-8