دورية أكاديمية
Analysis of accessible chromatin landscape in the inner cell mass and trophectoderm of human blastocysts.
| العنوان: | Analysis of accessible chromatin landscape in the inner cell mass and trophectoderm of human blastocysts. |
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| المؤلفون: | Yang M; The Foundation for Embryonic Competence, Basking Ridge, NJ, USA., Tao X; The Foundation for Embryonic Competence, Basking Ridge, NJ, USA., Titus S; The Foundation for Embryonic Competence, Basking Ridge, NJ, USA., Zhao T; IVI-RMA, New Jersey, Basking Ridge, NJ, USA., Scott RT; IVI-RMA, New Jersey, Basking Ridge, NJ, USA.; Department of Obstetrics and Gynecology, Thomas Jefferson University, Philadelphia, PA, USA., Seli E; IVI-RMA, New Jersey, Basking Ridge, NJ, USA.; Department of Obstetrics, Gynecology and Reproductive Sciences, Yale School of Medicine, New Haven, CT, USA. |
| المصدر: | Molecular human reproduction [Mol Hum Reprod] 2020 Sep 01; Vol. 26 (9), pp. 702-711. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press for the European Society for Human Reproduction and Embryology Country of Publication: England NLM ID: 9513710 Publication Model: Print Cited Medium: Internet ISSN: 1460-2407 (Electronic) Linking ISSN: 13609947 NLM ISO Abbreviation: Mol Hum Reprod Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oxford : Oxford University Press for the European Society for Human Reproduction and Embryology Original Publication: Oxford, UK : Published for the European Society for Human Reproduction and Embryology by Oxford University Press, [1995- |
| مواضيع طبية MeSH: | Blastocyst Inner Cell Mass/*metabolism , Chromatin/*metabolism , Ectoderm/*metabolism, Adult ; Blastocyst/chemistry ; Blastocyst/metabolism ; Blastocyst Inner Cell Mass/chemistry ; Cells, Cultured ; Chromatin/chemistry ; Chromatin Assembly and Disassembly/physiology ; DNA, Intergenic/analysis ; DNA, Intergenic/metabolism ; Ectoderm/chemistry ; Embryonic Development/genetics ; Female ; Humans ; Promoter Regions, Genetic/genetics |
| مستخلص: | Early embryonic development is characterized by drastic changes in chromatin structure that affects the accessibility of the chromatin. In human, the chromosome reorganization and its involvement in the first linage segregation are poorly characterized due to the difficulties in obtaining human embryonic material and limitation on low input technologies. In this study, we aimed to explore the chromatin remodeling pattern in human preimplantation embryos and gain insight into the epigenetic regulation of inner cell mass (ICM) and trophectoderm (TE) differentiation. We optimized ATAC-seq (an assay for transposase-accessible chromatin using sequencing) to analyze the chromatin accessibility landscape for low DNA input. Sixteen preimplantation human blastocysts frozen on Day 6 were used. Our data showed that ATAC peak distributions of the promoter regions (<1 kb) and distal regions versus other regions were significantly different between ICM versus TE samples (P < 0.01). We detected that a higher percentage of accessible binding loci were located within 1 kb of the transcription start site in ICM compared to TE (P < 0.01). However, a higher percentage of accessible regions was detected in the distal region of TE compared to ICM (P < 0.01). In addition, eight differential peaks with a false discovery rate <0.05 between ICM and TE were detected. This is the first study to compare the landscape of the accessible chromatin between ICM and TE of human preimplantation embryos, which unveiled chromatin-level epigenetic regulation of cell lineage specification in early embryo development. (© The Author(s) 2020. Published by Oxford University Press on behalf of European Society of Human Reproduction and Embryology. All rights reserved. For permissions, please email: journals.permissions@oup.com.) |
| فهرسة مساهمة: | Keywords: ATAC-seq; cell lineage; chromatin accessibility; human; inner cell mass; pre-implantation embryo; preimplantation embryo; trophectoderm |
| المشرفين على المادة: | 0 (Chromatin) 0 (DNA, Intergenic) |
| تواريخ الأحداث: | Date Created: 20200715 Date Completed: 20210826 Latest Revision: 20210826 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1093/molehr/gaaa048 |
| PMID: | 32663300 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1460-2407 |
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| DOI: | 10.1093/molehr/gaaa048 |