دورية أكاديمية
The sputum microbiome is distinct between COPD and health, independent of smoking history.
| العنوان: | The sputum microbiome is distinct between COPD and health, independent of smoking history. |
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| المؤلفون: | Haldar K; Institute for Lung Health, NIHR, BRC, Department of Respiratory Sciences, College of Life Sciences, University of Leicester, Leicester, LE1 7RH, UK., George L; Institute for Lung Health, NIHR, BRC, Department of Respiratory Sciences, College of Life Sciences, University of Leicester, Leicester, LE1 7RH, UK., Wang Z; Institute of Ecological Science, School of Life Science, South China Normal University, Guangzhou, 510631, China., Mistry V; Institute for Lung Health, NIHR, BRC, Department of Respiratory Sciences, College of Life Sciences, University of Leicester, Leicester, LE1 7RH, UK., Ramsheh MY; Institute for Lung Health, NIHR, BRC, Department of Respiratory Sciences, College of Life Sciences, University of Leicester, Leicester, LE1 7RH, UK., Free RC; Institute for Lung Health, NIHR, BRC, Department of Respiratory Sciences, College of Life Sciences, University of Leicester, Leicester, LE1 7RH, UK., John C; Department of Health Sciences, NIHR, BRC, University of Leicester, Leicester, LE1 7RH, UK., Reeve NF; Department of Health Sciences, NIHR, BRC, University of Leicester, Leicester, LE1 7RH, UK., Miller BE; Global Medical, GSK, Collegeville, PA, 19426, USA., Tal-Singer R; Global Medical, GSK, Collegeville, PA, 19426, USA., Webb AJ; Department of Genetics, University of Leicester, Leicester, LE1 7RH, UK., Brookes AJ; Department of Genetics, University of Leicester, Leicester, LE1 7RH, UK., Tobin MD; Department of Health Sciences, NIHR, BRC, University of Leicester, Leicester, LE1 7RH, UK., Singh D; University of Manchester and University Hospital of South Manchester, Manchester, M23 9QZ, UK., Donaldson GC; National Heart and Lung Institute, Imperial College London, London, SW3 6NP, UK., Wedzicha JA; National Heart and Lung Institute, Imperial College London, London, SW3 6NP, UK., Brown JR; Computational Biology, Human Genetics, Research and Development (R&D), GlaxoSmithKline (GSK), Collegeville, PA, 19426, USA., Barer MR; Institute for Lung Health, NIHR, BRC, Department of Respiratory Sciences, College of Life Sciences, University of Leicester, Leicester, LE1 7RH, UK., Brightling CE; Institute for Lung Health, NIHR, BRC, Department of Respiratory Sciences, College of Life Sciences, University of Leicester, Leicester, LE1 7RH, UK. ceb17@le.ac.uk.; Institute for Lung Health, University Hospitals of Leicester, Groby Road, Leicester, LE3 9QP, UK. ceb17@le.ac.uk. |
| المصدر: | Respiratory research [Respir Res] 2020 Jul 14; Vol. 21 (1), pp. 183. Date of Electronic Publication: 2020 Jul 14. |
| نوع المنشور: | Comparative Study; Journal Article; Multicenter Study |
| اللغة: | English |
| بيانات الدورية: | Publisher: BioMed Central Ltd Country of Publication: England NLM ID: 101090633 Publication Model: Electronic Cited Medium: Internet ISSN: 1465-993X (Electronic) Linking ISSN: 14659921 NLM ISO Abbreviation: Respir Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2001- : London : BioMed Central Ltd. Original Publication: London : Current Science Ltd., c2000- |
| مواضيع طبية MeSH: | Non-Smokers* , Smokers*, Lung/*microbiology , Pulmonary Disease, Chronic Obstructive/*microbiology , Sputum/*microbiology, Aged ; Case-Control Studies ; Dysbiosis ; England ; Female ; Health Status ; Humans ; Male ; Middle Aged ; Pulmonary Disease, Chronic Obstructive/diagnosis ; Ribotyping |
| مستخلص: | Background: Airway bacterial dysbiosis is a feature of chronic obstructive pulmonary disease (COPD). However, there is limited comparative data of the lung microbiome between healthy smokers, non-smokers and COPD. Methods: We compared the 16S rRNA gene-based sputum microbiome generated from pair-ended Illumina sequencing of 124 healthy subjects (28 smokers and 96 non-smokers with normal lung function), with single stable samples from 218 COPD subjects collected from three UK clinical centres as part of the COPDMAP consortium. Results: In healthy subjects Firmicutes, Bacteroidetes and Actinobacteria were the major phyla constituting 88% of the total reads, and Streptococcus, Veillonella, Prevotella, Actinomyces and Rothia were the dominant genera. Haemophilus formed only 3% of the healthy microbiome. In contrast, Proteobacteria was the most dominant phylum accounting for 50% of the microbiome in COPD subjects, with Haemophilus and Moraxella at genus level contributing 25 and 3% respectively. There were no differences in the microbiome profile within healthy and COPD subgroups when stratified based on smoking history. Principal coordinate analysis on operational taxonomic units showed two distinct clusters, representative of healthy and COPD subjects (PERMANOVA, p = 0·001). Conclusion: The healthy and COPD sputum microbiomes are distinct and independent of smoking history. Our results underline the important role for Gammaproteobacteria in COPD. |
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| معلومات مُعتمدة: | G0800570 United Kingdom MRC_ Medical Research Council; G0601369 United Kingdom MRC_ Medical Research Council; FP7-270194 Airprom; COPDMaP United Kingdom MRC_ Medical Research Council; MR/P00167X/1 United Kingdom MRC_ Medical Research Council; United Kingdom WT_ Wellcome Trust; RP-PG-0109-10056 United Kingdom DH_ Department of Health |
| فهرسة مساهمة: | Keywords: COPD; Haemophilus; Healthy airway; Microbiome; Proteobacteria |
| تواريخ الأحداث: | Date Created: 20200716 Date Completed: 20210609 Latest Revision: 20220323 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC7362436 |
| DOI: | 10.1186/s12931-020-01448-3 |
| PMID: | 32664956 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1465-993X |
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| DOI: | 10.1186/s12931-020-01448-3 |