دورية أكاديمية
أعرض في EDS

Characterization of a female germline and early zygote promoter from the transcription factor bZip1 in the dengue mosquito Aedes aegypti.

التفاصيل البيبلوغرافية
العنوان: Characterization of a female germline and early zygote promoter from the transcription factor bZip1 in the dengue mosquito Aedes aegypti.
المؤلفون: Kojin BB; Department of Entomology and Agrilife Research, Texas A&M University, College Station, TX, USA., Biedler JK; Department of Biochemistry and the Fralin Life Sciences Institute, Virginia Tech, Blacksburg, VA, USA., Tu Z; Department of Biochemistry and the Fralin Life Sciences Institute, Virginia Tech, Blacksburg, VA, USA., Adelman ZN; Department of Entomology and Agrilife Research, Texas A&M University, College Station, TX, USA. zachadel@tamu.edu.
المصدر: Parasites & vectors [Parasit Vectors] 2020 Jul 17; Vol. 13 (1), pp. 353. Date of Electronic Publication: 2020 Jul 17.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101462774 Publication Model: Electronic Cited Medium: Internet ISSN: 1756-3305 (Electronic) Linking ISSN: 17563305 NLM ISO Abbreviation: Parasit Vectors Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central
مواضيع طبية MeSH: Aedes/*genetics , Basic-Leucine Zipper Transcription Factors/*genetics , Mosquito Vectors/*genetics, Adult Germline Stem Cells/metabolism ; Animals ; Animals, Genetically Modified/genetics ; Basic-Leucine Zipper Transcription Factors/metabolism ; Dengue/transmission ; Female ; Promoter Regions, Genetic ; Regulatory Sequences, Ribonucleic Acid/genetics ; Transcription Factors/genetics ; Transcription Factors/metabolism ; Transgenes ; Zygote/metabolism
مستخلص: Background: The wide distribution of Aedes aegypti, the main vector of dengue and yellow fever viruses, currently puts three billion people in the world at risk of infection with these viruses. Continuous transmission of these and other viruses despite aggressive efforts to prevent this emphasizes the need to develop new control strategies. Proposals to control disease transmission based on vector engineering, including both population suppression and population replacement, rely on the development of transgenes under the control of regulatory elements able to drive molecules in a specific tissue, time and strength.
Methods: Here we report the characterization of a promoter active in both the female germline and early zygote, derived from the transcription factor bZip1 in the mosquito Ae. aegypti, using transposon-based methods and RT-qPCR.
Results: We generated seven transgenic lines carrying AabZip1-reporter constructs and observed expression in both the ovary and early embryo. RT-qPCR analysis was performed to evaluate transcript expression patterns for each line, confirming that transgenic expression from the AabZip1 promoter largely recapitulated the endogenous expression pattern, albeit the strength of maternal expression appeared to be strongly influenced by chromosomal position.
Conclusions: This study provides a new regulatory sequence that can be useful for generating transgenic lines that can become a tool in vector control strategies.
References: Bioinformatics. 2014 Apr 1;30(7):923-30. (PMID: 24227677)
Nucleic Acids Res. 2015 Jan;43(Database issue):D707-13. (PMID: 25510499)
Microbes Infect. 2018 Dec;20(11-12):646-660. (PMID: 29481868)
Insect Biochem Mol Biol. 2005 Jul;35(7):789-98. (PMID: 15894194)
Parasit Vectors. 2018 Dec 24;11(Suppl 2):655. (PMID: 30583735)
G3 (Bethesda). 2014 Dec 05;5(2):157-66. (PMID: 25480960)
Science. 2007 Apr 27;316(5824):597-600. (PMID: 17395794)
Annu Rev Entomol. 2007;52:209-29. (PMID: 16913829)
Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5547-51. (PMID: 1319065)
Sci Rep. 2013;3:1603. (PMID: 23549343)
Insect Mol Biol. 2010 Apr;19(2):263-9. (PMID: 20113372)
PLoS One. 2012;7(3):e33933. (PMID: 22457801)
Proc Natl Acad Sci U S A. 2007 Jun 12;104(24):9970-5. (PMID: 17548819)
Parasit Vectors. 2018 Dec 24;11(Suppl 2):652. (PMID: 30583723)
Neotrop Entomol. 2017 Jun;46(3):243-255. (PMID: 28401481)
Annu Rev Entomol. 2020 Jan 7;65:313-332. (PMID: 31594410)
Int J Biochem Cell Biol. 2017 Oct;91(Pt B):145-155. (PMID: 28673892)
Annu Rev Entomol. 2008;53:273-91. (PMID: 17803458)
Trends Parasitol. 2011 Aug;27(8):362-70. (PMID: 21659002)
Insect Mol Biol. 2010 Aug;19(4):441-9. (PMID: 20456509)
BMC Evol Biol. 2010 Jul 08;10:206. (PMID: 20615250)
Parasit Vectors. 2018 Dec 24;11(Suppl 2):644. (PMID: 30583731)
Antiviral Res. 2010 Feb;85(2):328-45. (PMID: 19857523)
Nucleic Acids Res. 2012 Aug;40(15):e115. (PMID: 22730293)
Malar J. 2013 Apr 26;12:142. (PMID: 23622561)
ACS Synth Biol. 2014 Dec 19;3(12):915-28. (PMID: 23654248)
Insect Biochem Mol Biol. 2006 Apr;36(4):273-81. (PMID: 16551541)
Nat Biotechnol. 2005 Apr;23(4):453-6. (PMID: 15750586)
Trends Parasitol. 2005 Feb;21(2):64-7. (PMID: 15664528)
Science. 2015 Jun 12;348(6240):1268-70. (PMID: 25999371)
Proc Natl Acad Sci U S A. 2013 Apr 30;110(18):7176-81. (PMID: 23584017)
BMC Biol. 2007 Mar 20;5:11. (PMID: 17374148)
Nat Biotechnol. 2018 Dec;36(11):1062-1066. (PMID: 30247490)
Proc Natl Acad Sci U S A. 1996 Aug 20;93(17):8934-9. (PMID: 8799131)
J Gen Virol. 2001 May;82(Pt 5):1027-1041. (PMID: 11297677)
Proc Natl Acad Sci U S A. 1998 Mar 31;95(7):3748-51. (PMID: 9520438)
Nat Biotechnol. 1999 Oct;17(10):969-73. (PMID: 10504696)
Bioinformatics. 2007 May 15;23(10):1289-91. (PMID: 17379693)
Anal Biochem. 2002 Mar 1;302(1):52-9. (PMID: 11846375)
G3 (Bethesda). 2013 Sep 04;3(9):1493-509. (PMID: 23833213)
Nat Methods. 2013 May;10(5):407-9. (PMID: 23524392)
Sci Rep. 2014 Feb 04;4:3954. (PMID: 24492376)
معلومات مُعتمدة: R01 AI123338 United States AI NIAID NIH HHS
فهرسة مساهمة: Keywords: Embryo; Germline; Maternal expression; Mosquito; Ovaries; Promoter; Regulatory sequence; Zygote
المشرفين على المادة: 0 (Basic-Leucine Zipper Transcription Factors)
0 (Regulatory Sequences, Ribonucleic Acid)
0 (Transcription Factors)
تواريخ الأحداث: Date Created: 20200719 Date Completed: 20210316 Latest Revision: 20240731
رمز التحديث: 20240731
مُعرف محوري في PubMed: PMC7367395
DOI: 10.1186/s13071-020-04216-w
PMID: 32680549
قاعدة البيانات: MEDLINE
الوصف
تدمد:1756-3305
DOI:10.1186/s13071-020-04216-w