دورية أكاديمية
IL-10 treatment decreases blood pressure in male, but not female, spontaneously hypertensive rats.
| العنوان: | IL-10 treatment decreases blood pressure in male, but not female, spontaneously hypertensive rats. |
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| المؤلفون: | Gillis EE; Department of Physiology, Augusta University, Augusta, Georgia., Musall JB; Department of Physiology, Augusta University, Augusta, Georgia., Baban B; Department of Oral Biology, Augusta University, Augusta, Georgia., Sullivan JC; Department of Physiology, Augusta University, Augusta, Georgia. |
| المصدر: | American journal of physiology. Renal physiology [Am J Physiol Renal Physiol] 2020 Sep 01; Vol. 319 (3), pp. F359-F365. Date of Electronic Publication: 2020 Jul 20. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Physiological Society Country of Publication: United States NLM ID: 100901990 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1522-1466 (Electronic) Linking ISSN: 15221466 NLM ISO Abbreviation: Am J Physiol Renal Physiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Bethesda, Md. : American Physiological Society, c1997- |
| مواضيع طبية MeSH: | Blood Pressure/*drug effects , Interleukin-10/*pharmacology, Animals ; Female ; Gene Expression Regulation/drug effects ; Inflammation/metabolism ; Infusion Pumps ; Infusions, Subcutaneous ; Interleukin-10/administration & dosage ; Kidney/cytology ; Kidney/drug effects ; Kidney/metabolism ; Male ; Nitric Oxide Synthase/genetics ; Nitric Oxide Synthase/metabolism ; Random Allocation ; Rats ; Rats, Inbred SHR ; Receptors, Interleukin-10/genetics ; Receptors, Interleukin-10/metabolism ; Sex Factors ; T-Lymphocytes/cytology |
| مستخلص: | Interleukin-10 (IL-10) is an anti-inflammatory cytokine that induces nitric oxide (NO) production. IL-10 supplementation has been previously shown to lower blood pressure (BP) in male hypertensive mice, but the effect of exogenous IL-10 in hypertensive female rodents has not been studied. For the present study, we hypothesized that chronic infusion of IL-10 in hypertensive rats would lower BP concomitant with an increase in renal NO synthase (NOS) activity. Male and female spontaneously hypertensive rats (SHRs; 12 wk old) were randomized to receive IL-10 infusion by subcutaneous minipump (3.5 µg·kg -1 ·day -1 ) or serve as sham controls ( n = 4-6 rats per treatment per sex). BP was measured by tail cuff before and after 2 wk of treatment. Renal T cells and IL-10 were measured by flow cytometry, and NOS activity was determined by conversion of radiolabeled arginine to radiolabeled citrulline. Female SHRs had greater IL-10 + renal cells than male SHRs and greater expression of the IL-10 receptor at baseline. BP did not change in female SHRs treated with IL-10, but BP significantly decreased following IL-10 infusion in male SHRs. Contrary to our hypothesis, NOS enzymatic activity decreased with IL-10 treatment in the renal inner medulla and cortex of both sexes. Renal regulatory T cells also decreased in both sexes after IL-10 treatment. In conclusion, despite male SHRs having less IL-10 and IL-10 receptor expression in the kidney compared with female SHRs, exogenous IL-10 selectively decreased BP only in male SHRs. Furthermore, our data suggest that exogenous IL-10-induced decreases in BP in male SHRs are not dependent on upregulating renal NOS activity. |
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| معلومات مُعتمدة: | R01 HL127091 United States HL NHLBI NIH HHS; R01 HL127091 United States GF NIH HHS |
| فهرسة مساهمة: | Keywords: T cells; hypertension; nitric oxide synthase; spontaneously hypertensive rats |
| المشرفين على المادة: | 0 (Receptors, Interleukin-10) 130068-27-8 (Interleukin-10) EC 1.14.13.39 (Nitric Oxide Synthase) |
| تواريخ الأحداث: | Date Created: 20200721 Date Completed: 20201127 Latest Revision: 20210903 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC7509288 |
| DOI: | 10.1152/ajprenal.00206.2020 |
| PMID: | 32686523 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1522-1466 |
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| DOI: | 10.1152/ajprenal.00206.2020 |