دورية أكاديمية
In Vivo Human PSC-Derived Intestinal Organoids to Study Stem Cell Maintenance.
| العنوان: | In Vivo Human PSC-Derived Intestinal Organoids to Study Stem Cell Maintenance. |
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| المؤلفون: | Vales S; Department of Pediatric General and Thoracic Surgery, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA., Poling HM; Department of Pediatric General and Thoracic Surgery, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA., Sundaram N; Department of Pediatric General and Thoracic Surgery, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA., Helmrath MA; Department of Pediatric General and Thoracic Surgery, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.; Department of Pediatrics, University of Cincinnati, Cincinnati, OH, USA., Mahe MM; Department of Pediatric General and Thoracic Surgery, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA. maxime.mahe@inserm.fr.; Department of Pediatrics, University of Cincinnati, Cincinnati, OH, USA. maxime.mahe@inserm.fr.; Université de Nantes, Inserm, TENS, The Enteric Nervous System in Gut and Brain Diseases, IMAD, Nantes, Nantes Cedex 1, France. maxime.mahe@inserm.fr. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2020; Vol. 2171, pp. 201-214. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Intestinal Mucosa/*cytology , Intestinal Mucosa/*metabolism , Pluripotent Stem Cells/*cytology , Pluripotent Stem Cells/*metabolism, Animals ; Cell Differentiation/genetics ; Cell Differentiation/physiology ; Cell Proliferation/genetics ; Cell Proliferation/physiology ; Cells, Cultured ; Female ; Humans ; Male ; Mice ; Organoids/cytology ; Organoids/metabolism ; Stem Cells/cytology ; Stem Cells/metabolism |
| مستخلص: | Human intestinal organoids (HIOs), derived from pluripotent stem cells, are a new tool to gain insights in gastrointestinal development, physiology, and associated diseases. Herein, we present a method for renal transplantation of HIOs in immunocompromised mice and subsequent analysis to study intestinal epithelial cell proliferation. In addition, we describe how to generate enteroids from transplanted HIOs. The method highlights the specific steps to successful engraftment and provides insight into the study of human intestinal stem cells. |
| فهرسة مساهمة: | Keywords: Enteroid; Human intestinal organoids; In vivo model; Intestinal stem cell; Pluripotent stem cells; Transplantation |
| تواريخ الأحداث: | Date Created: 20200725 Date Completed: 20210311 Latest Revision: 20210311 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1007/978-1-0716-0747-3_12 |
| PMID: | 32705643 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-6029 |
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| DOI: | 10.1007/978-1-0716-0747-3_12 |