دورية أكاديمية
أعرض في EDS

Functionalisation of a heat-derived and bio-inert albumin hydrogel with extracellular matrix by air plasma treatment.

التفاصيل البيبلوغرافية
العنوان: Functionalisation of a heat-derived and bio-inert albumin hydrogel with extracellular matrix by air plasma treatment.
المؤلفون: Ong J; Department of Engineering, University of Cambridge, Trumpington Street, Cambridge, CB2 1PZ, UK. jo401@cam.ac.uk.; East of England Gastroenterology Speciality Training Program, Fulbourn, Cambridge, CB21 5XB, UK. jo401@cam.ac.uk.; Department of Medicine, National University of Singapore, Kent Ridge Road, Singapore, 119228, Singapore. jo401@cam.ac.uk., Zhao J; Department of Engineering, University of Cambridge, Trumpington Street, Cambridge, CB2 1PZ, UK.; Duke-NUS Medical School, 8 College Road, Singapore, 169857, Singapore., Levy GK; Department of Engineering, University of Cambridge, Trumpington Street, Cambridge, CB2 1PZ, UK., Macdonald J; Department of Engineering, University of Cambridge, Trumpington Street, Cambridge, CB2 1PZ, UK., Justin AW; Department of Engineering, University of Cambridge, Trumpington Street, Cambridge, CB2 1PZ, UK., Markaki AE; Department of Engineering, University of Cambridge, Trumpington Street, Cambridge, CB2 1PZ, UK. am253@cam.ac.uk.
المصدر: Scientific reports [Sci Rep] 2020 Jul 24; Vol. 10 (1), pp. 12429. Date of Electronic Publication: 2020 Jul 24.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Plasma Gases*, Biocompatible Materials/*chemistry , Hydrogels/*chemistry , Serum Albumin, Human/*chemistry , Tissue Engineering/*methods, Biocompatible Materials/toxicity ; Cell Line ; Cell Proliferation/drug effects ; Extracellular Matrix Proteins/chemistry ; Extracellular Matrix Proteins/toxicity ; Extracellular Matrix Proteins/ultrastructure ; Hot Temperature ; Humans ; Hydrogels/toxicity ; Materials Testing ; Microscopy, Electron, Scanning ; Osteoblasts ; Serum Albumin, Human/toxicity ; Serum Albumin, Human/ultrastructure ; Sodium Chloride/chemistry ; Surface Properties
مستخلص: Albumin-based hydrogels are increasingly attractive in tissue engineering because they provide a xeno-free, biocompatible and potentially patient-specific platform for tissue engineering and drug delivery. The majority of research on albumin hydrogels has focused on bovine serum albumin (BSA), leaving human serum albumin (HSA) comparatively understudied. Different gelation methods are usually employed for HSA and BSA, and variations in the amino acid sequences of HSA and BSA exist; these account for differences in the hydrogel properties. Heat-induced gelation of aqueous HSA is the easiest method of synthesizing HSA hydrogels however hydrogel opacity and poor cell attachment limit their usefulness in downstream applications. Here, a solution to this problem is presented. Stable and translucent HSA hydrogels were created by controlled thermal gelation and the addition of sodium chloride. The resulting bio-inert hydrogel was then subjected to air plasma treatment which functionalised its surface, enabling the attachment of basement membrane matrix (Geltrex). In vitro survival and proliferation studies of foetal human osteoblasts subsequently demonstrated good biocompatibility of functionalised albumin hydrogels compared to untreated samples. Thus, air plasma treatment enables functionalisation of inert heat-derived HSA hydrogels with extracellular matrix proteins and these may be used as a xeno-free platform for biomedical research or cell therapy.
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المشرفين على المادة: 0 (Biocompatible Materials)
0 (Extracellular Matrix Proteins)
0 (Hydrogels)
0 (Plasma Gases)
451W47IQ8X (Sodium Chloride)
ZIF514RVZR (Serum Albumin, Human)
تواريخ الأحداث: Date Created: 20200726 Date Completed: 20201221 Latest Revision: 20210724
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC7382478
DOI: 10.1038/s41598-020-69301-7
PMID: 32709918
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-020-69301-7