دورية أكاديمية
Inhibitory effect of protein Y3 from Coprinus comatus on tobacco mosaic virus.
| العنوان: | Inhibitory effect of protein Y3 from Coprinus comatus on tobacco mosaic virus. |
|---|---|
| المؤلفون: | Xiao H; School of Life Science, Key Laboratory of Poyang Lake Environment and Resource, Ministry of Education, Nanchang University, Nanchang, Jiangxi 330031, China., Bian Y; School of Life Science, Key Laboratory of Poyang Lake Environment and Resource, Ministry of Education, Nanchang University, Nanchang, Jiangxi 330031, China., Huang H; School of Life Science, Key Laboratory of Poyang Lake Environment and Resource, Ministry of Education, Nanchang University, Nanchang, Jiangxi 330031, China., Zhang Z; School of Life Science, Key Laboratory of Poyang Lake Environment and Resource, Ministry of Education, Nanchang University, Nanchang, Jiangxi 330031, China., Wu L; School of Life Science, Key Laboratory of Poyang Lake Environment and Resource, Ministry of Education, Nanchang University, Nanchang, Jiangxi 330031, China. Electronic address: ncusk724@hotmail.com., Wu L; School of Life Science, Key Laboratory of Poyang Lake Environment and Resource, Ministry of Education, Nanchang University, Nanchang, Jiangxi 330031, China. Electronic address: lpwu000@ncu.edu.cn. |
| المصدر: | Pesticide biochemistry and physiology [Pestic Biochem Physiol] 2020 Sep; Vol. 168, pp. 104474. Date of Electronic Publication: 2019 Oct 23. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 1301573 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9939 (Electronic) Linking ISSN: 00483575 NLM ISO Abbreviation: Pestic Biochem Physiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York Ny : Academic Press |
| مواضيع طبية MeSH: | Coprinus* , Tobacco Mosaic Virus*, Plant Diseases ; Recombinant Proteins ; Nicotiana/virology |
| مستخلص: | The antiviral protein Y3 produced by the edible mushroom Coprinus comatus disrupts the tobacco mosaic virus (TMV) and inhibits the multiplication of TMV in Nicotiana tabacum; however, the detailed mechanism of its activity remains unclear. In this study, Y3 was demonstrated to interact with TMV coat protein (TMV-CP) in vitro as well as in tobacco plants by using a yeast two-hybrid system and bimolecular fluorescence complementation (BiFC). To explore the interaction site between Y3 and TMV-CP, the phenylalanine (Phe) at the 43rd and arginine (Arg) at the 55th amino acid of Y3 were individually converted to cysteine (Cys) and serine (Ser), named Y3T1 and Y3T2, respectively, and were then used in BiFC assays. Based on the information obtained about disulfide bonds in the protein structure, the two mutations were predicted to change the protein's disulfide bonds. The results showed Y3T1 lost the ability to interact with TMV-CP, suggesting that a specific Phe of Y3 is critical for the interaction between Y3 and CP in plants. Furthermore, a prokaryotic expression system was used to test the antiviral activities of protein Y3 (PY3) and two other mutated proteins (P-Y3T1, P-Y3T2). The results showed that recombinant protein P-Y3 had a slightly lower inhibitory effect against TMV than Y3 extracted directly from mushrooms; further, P-Y3T1 decreased antiviral activity in the tobacco plant significantly compared with P-Y3, suggesting that the anti-TMV effect of Y3 was directly related to the Y3-CP interaction. In contrast, P-Y3T2 was able to still interact with TMV-CP in the tobacco plant, and it increased the ability of the plant to resist TMV compared with PY3, indicating that PY3-T2 could be a candidate peptide for plant protection against TMV and that Y3 may have other inhibitory mechanisms against TMV in addition to its interaction with TMV-CP. (Copyright © 2019. Published by Elsevier Inc.) |
| فهرسة مساهمة: | Keywords: Coprinus comatus; Point mutation; Prokaryotic expression; Tobacco mosaic virus; Y3 protein |
| المشرفين على المادة: | 0 (Recombinant Proteins) |
| تواريخ الأحداث: | Date Created: 20200727 Date Completed: 20200728 Latest Revision: 20231213 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.pestbp.2019.09.012 |
| PMID: | 32711757 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1095-9939 |
|---|---|
| DOI: | 10.1016/j.pestbp.2019.09.012 |