دورية أكاديمية
أعرض في EDS

Dehydrodieugenol improved lung inflammation in an asthma model by inhibiting the STAT3/SOCS3 and MAPK pathways.

التفاصيل البيبلوغرافية
العنوان: Dehydrodieugenol improved lung inflammation in an asthma model by inhibiting the STAT3/SOCS3 and MAPK pathways.
المؤلفون: Santana FPR; Department of Biological Science, Federal University of São Paulo, Diadema, SP, Brazil; Department of Medicine, School of Medicine, University of São Paulo, SP, Brazil., da Silva RC; Department of Biological Science, Federal University of São Paulo, Diadema, SP, Brazil., Ponci V; Department of Biological Science, Federal University of São Paulo, Diadema, SP, Brazil., Pinheiro AJMCR; Universidade CEUMA, São Luís, MA, Brazil; Programa de Pós-Graduação da Rede BIONORTE, Brazil., Olivo CR; Department of Medicine, School of Medicine, University of São Paulo, SP, Brazil., Caperuto LC; Department of Biological Science, Federal University of São Paulo, Diadema, SP, Brazil., Arantes-Costa FM; Department of Medicine, School of Medicine, University of São Paulo, SP, Brazil., Claudio SR; Department of Bioscience, Federal University of São Paulo, Santos, Brazil., Ribeiro DA; Department of Bioscience, Federal University of São Paulo, Santos, Brazil., Tibério IFLC; Department of Medicine, School of Medicine, University of São Paulo, SP, Brazil., Lima-Neto LG; Universidade CEUMA, São Luís, MA, Brazil; Programa de Pós-Graduação da Rede BIONORTE, Brazil., Lago JHG; Center of Natural Sciences and Humanities, Federal University of ABC, Santo André, SP, Brazil., Prado CM; Department of Bioscience, Federal University of São Paulo, Santos, Brazil. Electronic address: carla.prado@unifesp.br.
المصدر: Biochemical pharmacology [Biochem Pharmacol] 2020 Oct; Vol. 180, pp. 114175. Date of Electronic Publication: 2020 Jul 24.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Science Country of Publication: England NLM ID: 0101032 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2968 (Electronic) Linking ISSN: 00062952 NLM ISO Abbreviation: Biochem Pharmacol Subsets: MEDLINE
أسماء مطبوعة: Publication: Oxford : Elsevier Science
Original Publication: Oxford, New York [etc.] Paragamon Press.
مواضيع طبية MeSH: Asthma/*drug therapy , Eugenol/*analogs & derivatives , Lignans/*therapeutic use , MAP Kinase Signaling System/*drug effects , Pneumonia/*drug therapy , STAT3 Transcription Factor/*antagonists & inhibitors , Suppressor of Cytokine Signaling 3 Protein/*antagonists & inhibitors, Animals ; Asthma/metabolism ; Dose-Response Relationship, Drug ; Eugenol/isolation & purification ; Eugenol/pharmacology ; Eugenol/therapeutic use ; Lauraceae ; Lignans/isolation & purification ; Lignans/pharmacology ; MAP Kinase Signaling System/physiology ; Male ; Mice ; Mice, Inbred BALB C ; Plant Extracts/isolation & purification ; Plant Extracts/pharmacology ; Plant Extracts/therapeutic use ; Pneumonia/metabolism ; RAW 264.7 Cells ; STAT3 Transcription Factor/metabolism ; Suppressor of Cytokine Signaling 3 Protein/metabolism
مستخلص: Background: Eugenol, a common phenylpropanoid derivative found in different plant species, has well-described anti-inflammatory effects associated with the development of occupational hypersensitive asthma. Dehydrodieugenol, a dimeric eugenol derivative, exhibits anti-inflammatory and antioxidant activities and can be found in the Brazilian plant species Nectandra leucantha (Lauraceae). The biological effects of dehydrodieugenol on lung inflammation remain unclear.
Purpose: This study aimed to investigate the effects of eugenol and dehydrodieugenol isolated from N. leucantha in an experimental model of asthma.
Methods: In the present work, the toxic effects of eugenol and dehydrodieugenol on RAW 264.7 cells and their oxidant and inflammatory effects before lipopolysaccharide (LPS) exposure were tested. Then, male BALB/c mice were sensitized with ovalbumin through a 29-day protocol and treated with vehicle, eugenol, dehydrodieugenol or dexamethasone for eight days beginning on the 22nd day until the end of the protocol. Lung function; the inflammatory profile; and the protein expression of ERK1/2, JNK, p38, VAChT, STAT3, and SOCS3 in the lung were evaluated by immunoblotting.
Results: Eugenol and dehydrodieugenol were nontoxic to cells. Both compounds inhibited NO release and the gene expression of IL-1β and IL-6 in LPS-stimulated RAW 264.7 cells. In OVA-sensitized animals, dehydrodieugenol reduced lung inflammatory cell numbers and the lung concentrations of IL-4, IL-13, IL-17, and IL-10. These anti-inflammatory effects were associated with inhibition of the JNK, p38 and ERK1/2, VAChT and STAT3/SOCS3 pathways. Moreover, treatment with dehydrodieugenol effectively attenuated airway hyperresponsiveness.
Conclusion: The obtained data demonstrate, for the first time, that dehydrodieugenol was more effective than eugenol in counteracting allergic airway inflammation in mice, especially its inhibition of the JNK, p38 and ERK1/2, components of MAPK pathway. Therefore, dehydrodieugenol can be considered a prototype for the development of new and effective agents for the treatment of asthmatic patients.
(Copyright © 2020 Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: Asthma; Dehydrodieugenol; Eugenol; MAPK; STAT
المشرفين على المادة: 0 (Lignans)
0 (Plant Extracts)
0 (STAT3 Transcription Factor)
0 (Socs3 protein, mouse)
0 (Stat3 protein, mouse)
0 (Suppressor of Cytokine Signaling 3 Protein)
0 (dehydrodieugenol)
3T8H1794QW (Eugenol)
تواريخ الأحداث: Date Created: 20200728 Date Completed: 20201231 Latest Revision: 20201231
رمز التحديث: 20221213
DOI: 10.1016/j.bcp.2020.114175
PMID: 32717226
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-2968
DOI:10.1016/j.bcp.2020.114175