دورية أكاديمية
أعرض في EDS

Survival of Lawsonia intracellularis in porcine peripheral blood monocyte-derived macrophages.

التفاصيل البيبلوغرافية
العنوان: Survival of Lawsonia intracellularis in porcine peripheral blood monocyte-derived macrophages.
المؤلفون: Pereira CER; Department of Veterinary Clinic and Surgery, Veterinary School, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.; Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota, United States of America., Resende TP; Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota, United States of America., Armién AG; Department of Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota, United States of America.; Ultrastructural Pathology Unit, Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota, United States of America., Laub RP; Department of Veterinary Clinic and Surgery, Veterinary School, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Vannucci FA; Department of Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota, United States of America., Santos RL; Department of Veterinary Clinic and Surgery, Veterinary School, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Gebhart CJ; Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota, United States of America.; Department of Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota, United States of America., Guedes RMC; Department of Veterinary Clinic and Surgery, Veterinary School, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
المصدر: PloS one [PLoS One] 2020 Jul 31; Vol. 15 (7), pp. e0236887. Date of Electronic Publication: 2020 Jul 31 (Print Publication: 2020).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Lawsonia Bacteria*/growth & development , Lawsonia Bacteria*/ultrastructure, Desulfovibrionaceae Infections/*veterinary , Macrophages/*microbiology, Animals ; Cell Line ; Intestinal Diseases/microbiology ; Intestinal Diseases/veterinary ; Phagocytosis ; Swine ; Swine Diseases/microbiology
مستخلص: Lawsonia intracellularis, an obligately intracellular enteric bacterium, infects intestinal epithelial cells, but may also be found within macrophages in the intestinal lamina propria of affected pigs. Macrophages play an important role in host defense against infectious agents, but the role of this cell in L. intracellularis infection is not well understood. The aim of this study was to evaluate the permissibility of macrophages to L. intracellularis infection in vitro. Pure culture of L. intracellularis was added to swine peripheral blood monocyte-derived macrophages. Viability of intracytoplasmic L. intracellularis was evaluated at different time points by transmission electron microscopy (TEM). Potential replication of L. intracellularis in macrophages was also evaluated by qPCR. By TEM, phagocytosis L. intracellularis within of phagolysosomes were observed 1-hour post-infection (hpi) and bacterial structures in binary fission at 48 hpi. The number of intracellular bacteria was determined at 1, 4, 24, 48, and 72 hpi by qPCR in infected macrophages and compared to the number of intracellular bacteria from culture in McCoy cells. In both cell lines, the amount of L. intracellularis was decreased at 4 hpiand increased at 24 hpi. The number of intracellular bacteria continued to increase in McCoy cells over time. This is the first study showing interaction, survival and propagation of L. intracellularis in macrophages. These findings are critical to establish an experimental model for future studies of the pathogenesis of porcine proliferative enteropathy and the potential persistence of L. intracellularis in macrophages during chronic infections.
Competing Interests: The authors have declared that no competing interests exist.
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تواريخ الأحداث: Date Created: 20200801 Date Completed: 20200929 Latest Revision: 20200929
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC7394435
DOI: 10.1371/journal.pone.0236887
PMID: 32735621
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0236887