دورية أكاديمية
A mutation found in esophageal cancer alters integrin β4 mRNA splicing.
| العنوان: | A mutation found in esophageal cancer alters integrin β4 mRNA splicing. |
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| المؤلفون: | Kelly GT; Pulmonary and Endothelial Laboratory, University of Arizona College of Medicine-Phoenix, Department of Internal Medicine, Phoenix, AZ, USA. Electronic address: gabrielkelly@email.arizona.edu., Faraj R; Pulmonary and Endothelial Laboratory, University of Arizona College of Medicine-Phoenix, Department of Internal Medicine, Phoenix, AZ, USA. Electronic address: rfaraj@email.arizona.edu., Dai Z; Pulmonary and Endothelial Laboratory, University of Arizona College of Medicine-Phoenix, Department of Internal Medicine, Phoenix, AZ, USA. Electronic address: zhiyudai@email.arizona.edu., Cress AE; University of Arizona Cancer Center, University of Arizona, Tucson, AZ, USA. Electronic address: cress@email.arizona.edu., Wang T; Pulmonary and Endothelial Laboratory, University of Arizona College of Medicine-Phoenix, Department of Internal Medicine, Phoenix, AZ, USA. Electronic address: twang@email.arizona.edu. |
| المصدر: | Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2020 Aug 27; Vol. 529 (3), pp. 726-732. Date of Electronic Publication: 2020 Jul 19. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2104 (Electronic) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2002- >: San Diego, CA : Elsevier Original Publication: New York, Academic Press. |
| مواضيع طبية MeSH: | RNA Splicing*, Esophageal Neoplasms/*genetics , Integrin beta4/*genetics , RNA, Messenger/*genetics, Cell Line, Tumor ; Humans ; Mutation ; RNA Splice Sites |
| مستخلص: | Integrin β4 (CD104, mRNA: ITGβ4) contributes to anchoring cells to the extracellular matrix and is regulated in many cancer types where it contributes to tumor progression. One splice variant, integrin β4E, is poorly characterized. We extracted several mutations from tumor samples within ITGB4 near the splice site that controls ITGβ4E production, and computational analysis predicted six of these would alter splicing to alter ITGβ4E abundance. One of these mutations, from an esophageal squamous cell carcinoma sample, was predicted to increase splicing toward ITGβ4E. We verified this effect using a minigene, and observed that integrin β4E slows esophageal squamous cell migration while other variants enhance migration, demonstrating that integrin β4E regulation through mutations may contribute to esophageal squamous cell tumorigenesis. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2020. Published by Elsevier Inc.) |
| معلومات مُعتمدة: | T32 HL007249 United States HL NHLBI NIH HHS; P01 HL134610 United States HL NHLBI NIH HHS |
| فهرسة مساهمة: | Keywords: Esophageal cancer; Hemidesmosome; ITGβ4; Integrin; Mutation; Splicing |
| المشرفين على المادة: | 0 (ITGB4 protein, human) 0 (Integrin beta4) 0 (RNA Splice Sites) 0 (RNA, Messenger) |
| تواريخ الأحداث: | Date Created: 20200802 Date Completed: 20210212 Latest Revision: 20210212 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.bbrc.2020.06.078 |
| PMID: | 32736699 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1090-2104 |
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| DOI: | 10.1016/j.bbrc.2020.06.078 |