دورية أكاديمية
Purification, Toxicity and Functional Characterization of a New Proteinaceous Mussel Biotoxin from Bizerte Lagoon.
العنوان: | Purification, Toxicity and Functional Characterization of a New Proteinaceous Mussel Biotoxin from Bizerte Lagoon. |
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المؤلفون: | Marrouchi R; Institut Pasteur de Tunis, Laboratoire des Venins et Biomolécules Thérapeutiques, Université Tunis El Manar, 13 Place Pasteur, B.P. 74, 1002 Tunis-Belvédère, Tunisia., Benoit E; CEA, Institut des Sciences du Vivant Frédéric Joliot, Département Médicaments et Technologies pour la Santé (DMTS), Service d'Ingénierie Moléculaire pour la Santé (SIMoS), Université Paris-Saclay, ERL 9004 CNRS/CEA, 91191 Gif sur Yvette, France., Schlumberger S; Institut des Neurosciences Paris-Saclay, UMR 9197 CNRS/Université Paris-Sud, CNRS, 91198 Gif sur Yvette, France., Marzougui Z; Institut Pasteur de Tunis, Laboratoire des Venins et Biomolécules Thérapeutiques, Université Tunis El Manar, 13 Place Pasteur, B.P. 74, 1002 Tunis-Belvédère, Tunisia., Le Caer JP; CNRS, Centre de Recherche de Gif-FRC3115, Institut de Chimie des Substances Naturelles, 91198 Gif sur Yvette CEDEX, France., Molgó J; CEA, Institut des Sciences du Vivant Frédéric Joliot, Département Médicaments et Technologies pour la Santé (DMTS), Service d'Ingénierie Moléculaire pour la Santé (SIMoS), Université Paris-Saclay, ERL 9004 CNRS/CEA, 91191 Gif sur Yvette, France., Kharrat R; Institut Pasteur de Tunis, Laboratoire des Venins et Biomolécules Thérapeutiques, Université Tunis El Manar, 13 Place Pasteur, B.P. 74, 1002 Tunis-Belvédère, Tunisia. |
المصدر: | Toxins [Toxins (Basel)] 2020 Jul 30; Vol. 12 (8). Date of Electronic Publication: 2020 Jul 30. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101530765 Publication Model: Electronic Cited Medium: Internet ISSN: 2072-6651 (Electronic) Linking ISSN: 20726651 NLM ISO Abbreviation: Toxins (Basel) Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Basel : MDPI |
مواضيع طبية MeSH: | Marine Toxins*/chemistry , Marine Toxins*/isolation & purification , Marine Toxins*/toxicity , Mytilus*, Animals ; Cell Line, Tumor ; Estuaries ; Male ; Mice, Inbred C57BL ; Potassium Channels/physiology ; Sodium Channels/physiology ; Tunisia |
مستخلص: | The marine environment is known to be occupied by microorganisms. The potential toxicity of some of these marine microorganisms, that are capable of producing unknown biotoxins, has always been underestimated. Indeed, these biotoxins may be a threat to human health through the consumption of contaminated seafood and fish. For more than ten years, recurrent but atypical toxicity has been detected in mussels from Bizerte lagoon (North of Tunisia) during routine tests. In this study, we have isolated and characterized a new proteinaceous marine biotoxin, named Mussel Toxic Peptide (MTP). Using HPLC, electrophoresis and LC/MS studies, we showed that MTP has a protein characteristic UV-spectrum, can be visualized by protein specific reagents such as Coomassie, and has a molecular mass of 6.4 kDa. Patch-clamp experiments performed on cultured N18 neuroblastoma cells revealed that MTP (0.9-18 µM) markedly inhibited voltage-gated Na current, but was about 23 times less active in blocking voltage-gated K current at equimolar concentrations. To the best of our knowledge, this is the first time that a proteinaceous marine biotoxin with relatively high molecular mass is isolated and involved in the contamination of mussels harvested from shellfish farming areas. |
References: | Mar Drugs. 2018 May 29;16(6):. (PMID: 29844286) J Basic Microbiol. 1998;38(5-6):303-11. (PMID: 9871328) Appl Environ Microbiol. 2014 Jun;80(11):3315-20. (PMID: 24657868) J Nat Toxins. 2002 Dec;11(4):269-75. (PMID: 12503869) Mar Drugs. 2013 Nov 28;11(12):4724-40. (PMID: 24287956) Biochem Soc Trans. 2001 Aug;29(Pt 4):565-70. (PMID: 11498029) Nucleic Acids Res. 2004 Jan 1;32(Database issue):D593-4. (PMID: 14681489) Neuropharmacology. 2018 May 1;133:404-414. (PMID: 29474819) J Biol Chem. 1951 Nov;193(1):265-75. (PMID: 14907713) Science. 1999 Sep 3;285(5433):1505-10. (PMID: 10498537) Nat Prod Rep. 2015 Mar;32(3):411-35. (PMID: 25338021) Aquat Toxicol. 2007 Aug 1;83(4):254-62. (PMID: 17582518) Toxicon. 2010 Apr 1;55(4):805-10. (PMID: 19941880) Anal Biochem. 1977 May 1;79(1-2):544-52. (PMID: 68686) J Pept Sci. 2003 Nov-Dec;9(11-12):666-78. (PMID: 14658788) Toxicon. 2003 Sep;42(3):297-300. (PMID: 14559081) Toxicon. 2013 Dec 1;75:27-34. (PMID: 23954513) Int J Environ Res Public Health. 2019 Dec 31;17(1):. (PMID: 31906079) Toxins (Basel). 2018 Dec 08;10(12):. (PMID: 30544794) Mycopathologia. 2000 Jan;149(1):21-5. (PMID: 11227850) J Neurochem. 2008 Nov;107(4):952-63. (PMID: 18990115) Toxicon. 1999 Jan;37(1):77-83. (PMID: 9920482) FEMS Microbiol Lett. 2010 Nov;312(2):192-200. (PMID: 20875054) |
فهرسة مساهمة: | Keywords: K current; Na current; mussel toxicity; proteinaceous biotoxin |
المشرفين على المادة: | 0 (Marine Toxins) 0 (Potassium Channels) 0 (Sodium Channels) |
تواريخ الأحداث: | Date Created: 20200806 Date Completed: 20210308 Latest Revision: 20210308 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC7472388 |
DOI: | 10.3390/toxins12080487 |
PMID: | 32751635 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2072-6651 |
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DOI: | 10.3390/toxins12080487 |