دورية أكاديمية
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Purification, Toxicity and Functional Characterization of a New Proteinaceous Mussel Biotoxin from Bizerte Lagoon.

التفاصيل البيبلوغرافية
العنوان: Purification, Toxicity and Functional Characterization of a New Proteinaceous Mussel Biotoxin from Bizerte Lagoon.
المؤلفون: Marrouchi R; Institut Pasteur de Tunis, Laboratoire des Venins et Biomolécules Thérapeutiques, Université Tunis El Manar, 13 Place Pasteur, B.P. 74, 1002 Tunis-Belvédère, Tunisia., Benoit E; CEA, Institut des Sciences du Vivant Frédéric Joliot, Département Médicaments et Technologies pour la Santé (DMTS), Service d'Ingénierie Moléculaire pour la Santé (SIMoS), Université Paris-Saclay, ERL 9004 CNRS/CEA, 91191 Gif sur Yvette, France., Schlumberger S; Institut des Neurosciences Paris-Saclay, UMR 9197 CNRS/Université Paris-Sud, CNRS, 91198 Gif sur Yvette, France., Marzougui Z; Institut Pasteur de Tunis, Laboratoire des Venins et Biomolécules Thérapeutiques, Université Tunis El Manar, 13 Place Pasteur, B.P. 74, 1002 Tunis-Belvédère, Tunisia., Le Caer JP; CNRS, Centre de Recherche de Gif-FRC3115, Institut de Chimie des Substances Naturelles, 91198 Gif sur Yvette CEDEX, France., Molgó J; CEA, Institut des Sciences du Vivant Frédéric Joliot, Département Médicaments et Technologies pour la Santé (DMTS), Service d'Ingénierie Moléculaire pour la Santé (SIMoS), Université Paris-Saclay, ERL 9004 CNRS/CEA, 91191 Gif sur Yvette, France., Kharrat R; Institut Pasteur de Tunis, Laboratoire des Venins et Biomolécules Thérapeutiques, Université Tunis El Manar, 13 Place Pasteur, B.P. 74, 1002 Tunis-Belvédère, Tunisia.
المصدر: Toxins [Toxins (Basel)] 2020 Jul 30; Vol. 12 (8). Date of Electronic Publication: 2020 Jul 30.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101530765 Publication Model: Electronic Cited Medium: Internet ISSN: 2072-6651 (Electronic) Linking ISSN: 20726651 NLM ISO Abbreviation: Toxins (Basel) Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel : MDPI
مواضيع طبية MeSH: Marine Toxins*/chemistry , Marine Toxins*/isolation & purification , Marine Toxins*/toxicity , Mytilus*, Animals ; Cell Line, Tumor ; Estuaries ; Male ; Mice, Inbred C57BL ; Potassium Channels/physiology ; Sodium Channels/physiology ; Tunisia
مستخلص: The marine environment is known to be occupied by microorganisms. The potential toxicity of some of these marine microorganisms, that are capable of producing unknown biotoxins, has always been underestimated. Indeed, these biotoxins may be a threat to human health through the consumption of contaminated seafood and fish. For more than ten years, recurrent but atypical toxicity has been detected in mussels from Bizerte lagoon (North of Tunisia) during routine tests. In this study, we have isolated and characterized a new proteinaceous marine biotoxin, named Mussel Toxic Peptide (MTP). Using HPLC, electrophoresis and LC/MS studies, we showed that MTP has a protein characteristic UV-spectrum, can be visualized by protein specific reagents such as Coomassie, and has a molecular mass of 6.4 kDa. Patch-clamp experiments performed on cultured N18 neuroblastoma cells revealed that MTP (0.9-18 µM) markedly inhibited voltage-gated Na current, but was about 23 times less active in blocking voltage-gated K current at equimolar concentrations. To the best of our knowledge, this is the first time that a proteinaceous marine biotoxin with relatively high molecular mass is isolated and involved in the contamination of mussels harvested from shellfish farming areas.
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فهرسة مساهمة: Keywords: K current; Na current; mussel toxicity; proteinaceous biotoxin
المشرفين على المادة: 0 (Marine Toxins)
0 (Potassium Channels)
0 (Sodium Channels)
تواريخ الأحداث: Date Created: 20200806 Date Completed: 20210308 Latest Revision: 20210308
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC7472388
DOI: 10.3390/toxins12080487
PMID: 32751635
قاعدة البيانات: MEDLINE
الوصف
تدمد:2072-6651
DOI:10.3390/toxins12080487